Skin fibrosis . High‐Fluence Light Emitting Diode‐Red Light Inhibits Cell Cycle Progression in Human Dermal Fibroblasts

High‐Fluence Light Emitting Diode‐Red Light Inhibits Cell Cycle Progression in Human Dermal Fibroblasts:

Abstract

Skin fibrosis is a debilitating feature of several systemic and dermatologic diseases. While current treatment options carry significant risk of side effects and recurrence, high‐fluence light emitting diode‐generated red light (LED‐RL) is an alternative therapeutic that is safe, non‐invasive, and accessible. We previously demonstrated LED‐RL decreases fibroblast proliferation, a key pathogenic component of fibrosis. However, the cellular mechanism by which high fluence LED‐RL modulates fibroblast proliferation is unclear. Herein, we explored the effects of high fluence LED‐RL on human dermal fibroblast cell cycle progression. We demonstrate that LED‐RL at 640 J/cm² induced significant arrest of cells in G₀/G₁ compared to temperature‐matched control. This was accompanied by a corresponding increase in expression of checkpoint regulator p53 in irradiated cells. These data demonstrate high fluence LED‐RL may exert its anti‐proliferative effect on fibroblasts by inducing G₀/G₁ arrest. Further, this study provides insight into the molecular mechanism underlying LED‐RL as an anti‐fibrotic therapeutic.

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