FISH and chips: a review of microfluidic platforms for FISH analysis

FISH and chips: a review of microfluidic platforms for FISH analysis: AbstractFluorescence in situ hybridization (FISH) allows visualization of specific nucleic acid sequences within an intact cell or a tissue section. It is based on molecular recognition between a fluorescently labeled probe that penetrates the cell membrane of a fixed but intact sample and hybridizes to a nucleic acid sequence of interest within the cell, rendering a measurable signal. FISH has been applied to, for example, gene mapping, diagnosis of chromosomal aberrations and identification of pathogens in complex samples as well as detailed studies of cellular structure and function. However, FISH protocols are complex, they comprise of  many fixation, incubation and washing steps involving a range of solvents and temperatures and are, thus, generally time consuming and labor intensive...

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