BIOCHEMISTRY AND CHEMICAL BIOLOGY, CELL BIOLOGY, Release of cholesterol-rich particles from the macrophage plasma membrane during movement of filopodia and lamellipodia Xuchen Hu, Thomas A Weston ... Loren G Fong Macrophages release 30-nm vesicular particles enriched in accessible cholesterol during cellular locomotion, a discovery that is likely relevant to cholesterol disposal by these cells. DEVELOPMENTAL BIOLOGY, GENETICS AND GENOMICS Extensive intraspecies cryptic variation in an ancient embryonic gene regulatory network Yamila N Torres Cleuren, Chee Kiang Ewe ... Joel H Rothman Quantitative genetic analyses reveal remarkably broad genetic variation underlies the requirement for two critical regulatory inputs into a core embryonic gene regulatory network within one animal species. NEUROSCIENCE RIM is essential for stimulated but not spontaneous somatodendritic dopamine release in the midbrain Brooks G Robinson, Xintong Cai ... Pascal S Kaeser Midbrain dopamine neurons use sophisticated secretory machinery to establish specialized sites for action potential-evoked release of dopamine from their cell bodies and dendrites. CELL BIOLOGY, COMPUTATIONAL AND SYSTEMS BIOLOGY Remote control of microtubule plus-end dynamics and function from the minus-end Xiuzhen Chen, Lukas A Widmer ... Yves Barral A mechanism for yeast centrosomes to differentiate the microtubule cytoskeleton. DEVELOPMENTAL BIOLOGY, NEUROSCIENCE How prolonged expression of Hunchback, a temporal transcription factor, re-wires locomotor circuits Julia L Meng, Zarion D Marshall ... Ellie S Heckscher In vivo stem cell reprogramming in the well-studied stem cell NB7-1 using the classic temporal transcription factor Hunchback increases motor neuron number and re-specifies dendritic morphology and neuromuscular synaptic partnerships. EPIDEMIOLOGY AND GLOBAL HEALTH, STRUCTURAL BIOLOGY AND MOLECULAR BIOPHYSICS Electron cryo-microscopy of bacteriophage PR772 reveals the elusive vertex complex and the capsid architecture Hemanth KN Reddy, Marta Carroni ... Martin Svenda The structure and composition of the elusive vertex complex in Tectiviridae is finally revealed and the newly reported protein conformations help to maintain the capsid architecture. EVOLUTIONARY BIOLOGY Molecular function limits divergent protein evolution on planetary timescales Mariam M Konaté, Germán Plata ... Dennis Vitkup Orthologous proteins that continuously maintain the same molecular function do not usually diverge beyond a certain level of sequence and structural similarity. EVOLUTIONARY BIOLOGY Evolution of Yin and Yang isoforms of a chromatin remodeling subunit precedes the creation of two genes Wen Xu, Lijiang Long ... Patrick T McGrath Gene sharing through different isoforms can precede the formation of independent genes. DEVELOPMENTAL BIOLOGY, EVOLUTIONARY BIOLOGY A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle Glenda Comai, Eglantine Heude ... Shahragim Tajbakhsh Tbx1-Isl1-Met defines a unique genetic hierarchy that regulates esophagus myogenesis and patterning in the mouse. DEVELOPMENTAL BIOLOGY, EVOLUTIONARY BIOLOGY Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development Sruthi Purushothaman, Ahmed Elewa, Ashley W Seifert While movement of Fgf-signaling to the limb mesenchyme accompanied a shift in function, the ultimate outcome remains a convergent tetrapod limb phenotype. NEUROSCIENCE RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses Marisa M Brockmann, Marta Maglione ... Dietmar Schmitz The active zone scaffold protein RIM-BP2 performs distinct functions in vesicle release at two hippocampal synapses, providing insights on how synapses express diversity in release properties.

NEW ACCEPTED MANUSCRIPTS CHROMOSOMES AND GENE EXPRESSION, MICROBIOLOGY AND INFECTIOUS DISEASE Viral miRNA adaptor differentially recruits miRNAs to target mRNAs through alternative base-pairing Carlos Gorbea, Tim Mosbruger ... Demián Cazalla PHYSICS OF LIVING SYSTEMS Phylodynamic theory of persistence, extinction and speciation of rapidly adapting pathogens Le Yan, Richard A Neher, Boris I Shraiman PLANT BIOLOGY Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID Andrea Mair, Shou-ling Xu ... Dominique C Bergmann CELL BIOLOGY, GENETICS AND GENOMICS NHR-14 loss of function couples intestinal iron uptake with innate immunity in C. elegans through PQM-1 signaling Malini Rajan, Cole P Anderson ... Elizabeth A Leibold HUMAN BIOLOGY AND MEDICINE, IMMUNOLOGY AND INFLAMMATION ENaC-mediated sodium influx exacerbates NLRP3-dependent inflammation in Cystic Fibrosis Thomas Scambler, Heledd H Jarosz-Griffiths ... Michael F McDermott DEVELOPMENTAL BIOLOGY, STEM CELLS AND REGENERATIVE MEDICINE A distinct transition from cell growth to physiological homeostasis in the tendon Mor Grinstein, Heather L Dingwall ... Jenna Lauren Galloway NEUROSCIENCE ON selectivity in Drosophila vision is a multisynaptic process involving both glutamatergic and GABAergic inhibition Sebastian Molina-Obando, Juan Felipe Vargas-Fique ... Marion Silies PLANT BIOLOGY, STRUCTURAL BIOLOGY AND MOLECULAR BIOPHYSICS Protein engineering expands the effector recognition profile of a rice NLR immune receptor Juan Carlos De la Concepcion, Marina Franceschetti ... Mark J Banfield STRUCTURAL BIOLOGY AND MOLECULAR BIOPHYSICS Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands Jie Li, Guijun Shang ... Xiao-chen Bai COMPUTATIONAL AND SYSTEMS BIOLOGY, NEUROSCIENCE Rats exhibit similar biases in foraging and intertemporal choice tasks Gary A Kane, Aaron M Bornstein ... Jonathan D Cohen

DIGESTS DEVELOPMENTAL BIOLOGY, GENETICS AND GENOMICS One species, many ways to make a gut The inputs into the network of genes that controls how the gut develops vary dramatically between different members of a species of nematode. DEVELOPMENTAL BIOLOGY, EVOLUTIONARY BIOLOGY How the axolotl grows its arms The limbs of a salamander develop differently compared to those of other four-limbed animals such as frogs, chickens and mice. CELL BIOLOGY, PLANT BIOLOGY How roots direct their growth Two separate mechanisms control which direction cells in the roots of plants grow.