Contributors : Tao Cheng ; Hui Cheng ; Yajie WangSeries Type : Expression profiling by arrayOrganism : Mus musculusTo investigate the molecular mechanism for the elimination of leukemia cells after Dox induced OSKM expression, we sorted MLL-AF9 leukemia cells from Dox untreated and treated groups (48h and 72h) leukemic mice for gene expression profiling analysis.
16h
Contributors : Shannon Farris ; James M WardSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusRNA localization is one mechanism that neurons use to spatially and temporally regulate gene expression at synapses. Here, we tested the hypothesis that cells exhibiting distinct forms of synaptic plasticity will have differences in dendritically localized RNAs. Indeed, we discovered that each major subregion of the adult mouse hippocampus expresses a unique complement...
16h
Contributor : Hugh J FrenchSeries Type : Expression profiling by arrayOrganism : Mus musculusBecause activation of the immune response is dependent on extensive changes in gene expression, it is likely that a major component of inter-individual variation in the immune response is ultimately mediated at the level of gene regulation. Here, we examine the influence of genetic variation on inducible gene expression in the murine immune response. We extracted primary CD4 splenocytes from inbred strains...
16h
Series Type : Expression profiling by array ; Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
16h
Contributors : Farris Shannon ; Ward James M.Series Type : Expression profiling by arrayOrganism : Mus musculusRNA localization is one mechanism that neurons use to spatially and temporally regulate gene expression at synapses. Here, we tested the hypothesis that cells exhibiting distinct forms of synaptic plasticity will have differences in dendritically localized RNAs. Indeed, we discovered that each major subregion of the adult mouse hippocampus expresses a unique complement of dendritic RNAs....
16h
Contributor : Hugh J FrenchSeries Type : Expression profiling by arrayOrganism : Mus musculusBecause activation of the immune response is dependent on extensive changes in gene expression, it is likely that a major component of inter-individual variation in the immune response is ultimately mediated at the level of gene regulation. Here, we examine the influence of genetic variation on inducible gene expression in the murine immune response.We report strain-specific genome-wide differences in mRNA...
16h
Contributors : Lara K Abramowitz ; Chritelle Harly ; Avinash Bhandoola ; John A HanoverSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIn order to define HSC dysfunction in Oga mutant mice, we examined transcriptional deregulation in LSK cells from Oga(Vav-cre) mice in comparison to wildtype and found 41 downregulated and 12 upregulated genes
16h
Contributors : Zheng Chen ; Linna JiaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPurpose: The goal of this study is to investigate how Purβ regulates hepatic glucose production in primary hepatocytes.Methods: mRNA profiles of Purβ-overexpressing, and Purβ-knocking down hepatocytes were generated by deep sequencing using an Illumina NovaSeq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(Ensemble_GRCm38.96) with TopHat (version...
16h
Contributors : Jing Yang ; Lili ZhangSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensStable ectopic TCF12 expression or knockdown in HCC cell lines was established by lentiviral infection. Then, MTT, colony formation, migration, invasion and HUVEC tube formation assays as well as an orthotopic xenograft model were used to investigate the biologic function of TCF12 in HCC cells in vitro and in vivo. Subsequently, RNA-Seq analysis was utilized...
16h
Contributors : Jing Yang ; Lili ZhangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensStable ectopic TCF12 expression or knockdown in HCC cell lines was established by lentiviral infection. Then, MTT, colony formation, migration, invasion and HUVEC tube formation assays as well as an orthotopic xenograft model were used to investigate the biologic function of TCF12 in HCC cells in vitro and in vivo. Subsequently, RNA-Seq analysis was utilized to explore the...
16h
Contributors : Kazuhiro Ikeda ; Kuniko Horie-Inoue ; Satoshi InoueSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo examine the role of COX7RP in breast cancer cells, MCF7 cells, which were stably transfected with COX7RP (COX7RP-MCF7) or control vector (vector-MCF7), were cultured in normoxic and hypoxic condition. Microarray analysis showed that COX7RP modulates metabolism-associated genes.
16h
Contributors : Shuai Hou ; Haixin LeiSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe performed RNA-seq to determine the impact of XAB2 depletion on global splicing. The results show that XAB2 depletion using two different siRNAs led to a wide range of splicing defects, with more than 50% events were intron retention.
17h
Contributors : Chenggang Wang ; Xudong Zhang ; Jian L Li ; Yanping Zhang ; Zhonglin MouSeries Type : Expression profiling by arrayOrganism : Arabidopsis thalianaTo define how DRL1 regulates Botrytis cinerea-induced transcriptional changes at the genome level, we performed a microarray experiment on B. cinerea-infected drl1 and wild-type plants to identify genes that were differentially expressed between drl1 and the wild type. A total of 200 defense response genes were differentially expressed...
17h
Contributors : David M Suter ; Elias T FrimanSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusSOX2 and OCT4 are pioneer transcription factors playing a key role in embryonic stem (ES) cell self-renewal and differentiation. How temporal fluctuations in their expression levels bias lineage commitment is unknown. Here we generated knock-in reporter fusion ES cell lines allowing to monitor endogenous SOX2 and OCT4 protein fluctuations in living cells...
17h
Contributors : Shuo Chen ; Yun Tan ; Min LuSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensWe performed p53 ChIP-seq analysis of Nutlin-treated HCT116 cells to identify high-confident p53 regulated targets. And we performed ChIP-seq using an anti-p53 antibody in HCT116 cells treated with control or iASPP RNAi to identify iASPP regulated p53 targets.
17h
Contributor : Joseph MattapallilSeries Type : Expression profiling by arrayOrganism : Macaca mulattaQuestion Addressed: How does Simian immunodeficiency virus (SIV) infection alter gene expression in memory CD4 T cell subsets very early during the 1st 10 days after infection? Memory CD4 T cells were sorted from rhesus macaque peripheral blood samples before infection and then at 4 and 10 days post SIV infection. RNA was recovered from the sorted memory CD4 T cells samples. RNA from the time point...
17h
Contributor : Kawther AbdillehSeries Type : Expression profiling by high throughput sequencingOrganism : Labeotropheus fuelleborni ; Maylandia zebraWe use the continuously replacing dentition of Lake Malawi cichlid fishes to understand de-novo tooth replacement in adult vertebrates. In this system, each tooth is replaced in a one-for-one fashion every ~50 days. Here, we explore the source of epithelial stem cells for tooth replacement.
17h
Contributors : Fan Zou ; Lijuan LuSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo further development of different gene expression between the PTL-Her2-CAR-T cells (knockdown of PD-1, Tim-3, Lag-3 by shRNA-clusters in Her2-CAR-T cells) and Her2-CAR-T cells, we have employed whole genome microarray expression profiling as a discovery platform.
17h
Contributors : T Hailay ; M Hoelker ; M Poirier ; S Gebremedhn ; F Rings ; M Saeed Zidane ; D Salilew-Wondium ; C Dauben ; E Tholen ; C Neuhoff ; K Schellander ; D TesfayeSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Bos taurusThis study was performed to discover the association between postpartum during negative energy balance and the expression of extracellular vesicle-coupled microRNAs signatures in follicular fluid of large follicle in dairy cows....
17h
Contributors : Ryan F Bloomquist ; Teresa E Fowler ; Zhengwen An ; Tian Yu ; Kawther Abdileh ; Gareth J Fraser ; Paul T Sharpe ; J T StreelmanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIn Cichlids, replacement teeth (RT) share a continuous band of epithelium with adjacent taste buds (TB) and both organs co-express stem cell factors in subsets of label-retaining cells. In the mouse and other mammals, the tongue inter molar eminence (IE) oral papillae...
17h
Contributors : Zhen Tong ; Nocholas W Morrell ; Wei LiSeries Type : Expression profiling by arrayOrganism : Homo sapiensThis experiment was desinged to investigate the difference in global gene expression induced by the circulating form of BMP9, pro-BMP9, and BMP9 in complex with soluble endoglin (sENG:BMP9).
17h
Contributors : Igor Ulitsky ; Aviv RomSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencing ; OtherOrganism : Mus musculusLong noncoding RNAs (lncRNAs) are enriched in regions flanking transcription- and chromatin-associated genes, but the functional importance of such co-location events is largely unclear. Chromodomain helicase DNA binding protein 2 (Chd2) is chromatin remodeller with various reported functions in cell...
20h
Title: Can Aspirin Help Tackle Some Cancers?Category: Health NewsCreated: 9/23/2019 12:00:00 AMLast Editorial Review: 9/23/2019 12:00:00 AM
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