Therapeutic effects of metformin for noise induced hearing loss
Author links open overlay panelÖzgeGedikaRemziDoğanbMehmet AliBabademezcErsinKarataşdMehmet ŞerifAydıneAbdurrahimKoçyiğitfMukaddesEşrefoğlugOrhanÖzturanb
a
Bezmialem Vakif University, Faculty of Health Sciences, Audiology Department, Fatih, Istanbul, Turkey
b
Bezmialem Vakif University, Department of Otorhinolaryngology, Fatih, Istanbul, Turkey
c
Ankara Yildirim Beyazit University, Department of Otorhinolaryngology, Ankara, Turkey
d
Gebze Technical University, Faculty of Basic Sciences, Department of Moleculer Biology and Genetics, Kocaeli, Turkey
e
Istanbul Medipol University, Regenerative and Restorative Medicine Research Center, Beykoz, Istanbul, Turkey
f
Bezmialem Vakif University, Department of Basic Medical Sciences, Medical Biochemistry, Fatih, Istanbul, Turkey
g
Bezmialem Vakif University, Department of Basic Medical Sciences, Histology and Emryology, Fatih, Istanbul, Turkey
Received 4 October 2019, Available online 30 October 2019.
Abstract
Objective
This study aimed to investigate the healing effect of metformin on noise induced hearing loss (NIHL) by measuring audiological, biochemical and histological parameters.
Materials and methods
32 rats were divided into four groups (Group 1: Noise, Group 2: Noise + Metformin, Grup 3: Metformin, Grup 4: Control). Broadband noise was applied to Group 1 and Group 2 after basal measurements. Measuring audiological (distortion product otoacoustic emission (DPOAE) and Auditory Brainstem Response (ABR)), biochemical (total antioxidant status (TAS), total oxidant status (TOS), oxidative status index (OSI), DNA damage, IL-1 beta, IL-6, TNF alfa, HSF-1 and COX-2) and histological parameters.
Results
Group 2 had significant decreases in ABR thresholds on day 7 and day 14 compared to day 1. DPOAE values of Group 2 on the 7th and 14th days were significantly higher than the post-noise levels. DNA damage, TOS and OSI values of Group 1 were significantly higher than the other groups. The Cox-2 value of Group 1 was higher than all other groups. The HSF-1 value of Group 2 was significantly higher than that of Group 1. In terms of IL-1 Beta, IL-6 and TNF-alpha values, there was no significant difference between groups 2, 3 and 4 and these values were significantly lower than group 1. In histopathological results of our study, no significant difference was found between the groups being exposed to noise and the control group.
Conclusion
This study showed that early period of Metformin treatment has therapeutic effect on NIHL.
Keywords
ABRDNA damageDPOAENoise-induced hearing lossMetforminRat
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© 2019 Elsevier Inc. All rights reserved.
Author links open overlay panelÖzgeGedikaRemziDoğanbMehmet AliBabademezcErsinKarataşdMehmet ŞerifAydıneAbdurrahimKoçyiğitfMukaddesEşrefoğlugOrhanÖzturanb
a
Bezmialem Vakif University, Faculty of Health Sciences, Audiology Department, Fatih, Istanbul, Turkey
b
Bezmialem Vakif University, Department of Otorhinolaryngology, Fatih, Istanbul, Turkey
c
Ankara Yildirim Beyazit University, Department of Otorhinolaryngology, Ankara, Turkey
d
Gebze Technical University, Faculty of Basic Sciences, Department of Moleculer Biology and Genetics, Kocaeli, Turkey
e
Istanbul Medipol University, Regenerative and Restorative Medicine Research Center, Beykoz, Istanbul, Turkey
f
Bezmialem Vakif University, Department of Basic Medical Sciences, Medical Biochemistry, Fatih, Istanbul, Turkey
g
Bezmialem Vakif University, Department of Basic Medical Sciences, Histology and Emryology, Fatih, Istanbul, Turkey
Received 4 October 2019, Available online 30 October 2019.
Figures (6)
Abstract
Objective
This study aimed to investigate the healing effect of metformin on noise induced hearing loss (NIHL) by measuring audiological, biochemical and histological parameters.
Materials and methods
32 rats were divided into four groups (Group 1: Noise, Group 2: Noise + Metformin, Grup 3: Metformin, Grup 4: Control). Broadband noise was applied to Group 1 and Group 2 after basal measurements. Measuring audiological (distortion product otoacoustic emission (DPOAE) and Auditory Brainstem Response (ABR)), biochemical (total antioxidant status (TAS), total oxidant status (TOS), oxidative status index (OSI), DNA damage, IL-1 beta, IL-6, TNF alfa, HSF-1 and COX-2) and histological parameters.
Results
Group 2 had significant decreases in ABR thresholds on day 7 and day 14 compared to day 1. DPOAE values of Group 2 on the 7th and 14th days were significantly higher than the post-noise levels. DNA damage, TOS and OSI values of Group 1 were significantly higher than the other groups. The Cox-2 value of Group 1 was higher than all other groups. The HSF-1 value of Group 2 was significantly higher than that of Group 1. In terms of IL-1 Beta, IL-6 and TNF-alpha values, there was no significant difference between groups 2, 3 and 4 and these values were significantly lower than group 1. In histopathological results of our study, no significant difference was found between the groups being exposed to noise and the control group.
Conclusion
This study showed that early period of Metformin treatment has therapeutic effect on NIHL.
Keywords
ABRDNA damageDPOAENoise-induced hearing lossMetforminRat
View full text
© 2019 Elsevier Inc. All rights reserved.
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