Abstract In order to adapt to different environments, Vibrio parahaemolyticus employed a complicated quorum sensing system to orchestrate gene expression and diverse colony morphology patterns. In this study, the function of the putative quorum sensing signal synthase gene cqsA (VPA0711 in V. Parahaemolyticus strain RIMD2210633 genome) was investigated. The cloning and expression of V. parahaemolyticus cqsA in Escherichia coli system induced the production of a new quorum sensing...
02:00
Abstract A lectin gene (plectin) with a high level of expression was previously identified by comparative transcriptome analysis of Pleurotus ostreatus. In this study, we cloned a 733-bp DNA fragment from the start codon of the plectin gene. Sequence analysis showed that the plectin promoter (Plp) region contained several eukaryotic transcription factor binding motifs, such as the TATA-box, four possible CAAT-box, light responsiveness motifs and MeJA-responsiveness motifs. To determine...
02:00
Abstract A strictly anaerobic bacterium, designated as strain KGMB-03357T, was isolated from the faeces of a healthy Korean selected by Bundang Seoul National University based on health status. Cells of strain KGMB03357T are Gram-stain-positive, non-motile, non-spore-forming, and observed as straight or curved rods. The isolate grew at 10–45°C (optimum temperature of 40°C) and a pH range of 5.1–10.5 (optimum pH of 6.8). Analysis of phylogenetic trees based on the 16S rRNA gene...
02:00
Abstract One of the advantages for initial survival of inhaled fungal spores in the respiratory tract is the ability for iron acquisition via hemolytic factor-production. To examine the ability of indoor Aspergillus and Penicillium affecting hemolysis, the secreted factors during the growth of thirteen strains from eight species were characterized in vitro for their hemolytic activity (HA) and CAMP-like reaction. The hemolytic index of HA on human blood agar of Aspergillus micronesiensis,...
02:00
Abstract We propose a hemodynamic reduced‐order model bridging macroscopic and mesoscopic blood flow circulation scales from arteries to capillaries. In silico tree like vascular geometries, mathematically described by graphs, are synthetically generated by means of stochastic growth algorithms constrained by statistical morphological and topological principles. Scale‐specific pruning gradation of the tree is then proposed in order to fit computational budget requirement. Different compliant structural...
11:14
Abstract We investigated the performance of three tenodesis techniques, modified Brunelli (MBT), Corella and scapholunate axis (SLAM) methods in repairing scapholunate interosseous ligament (SLIL) disruption for a type II wrist using Finite Element based virtual surgery and compared the results to those of a previous investigation for a type I wrist. In addition, a comparison of the carpal mechanics of type I and type II wrists was undertaken in order to elucidate the difference between the two...
Sun Nov 03, 2019 18:58
Abstract Tortuous vessels are often observed in vivo and could hinder or even disrupt blood flow to distal organs. Beside genetic and biological factors, the in vivo mechanical loading seems to play a role in the formation of tortuous vessels but the mechanism for formation of helical vessel shape remains unclear. Accordingly, the aim of this study was to investigate the biomechanical loads that trigger the occurrence of helical buckling in blood vessels using finite element analysis (FEA). Porcine...
Sun Nov 03, 2019 17:27
Abstract Photothermal therapy using near‐infrared radiation and local heating agents can induce selective tumor ablation with limited harm to the surrounding normal tissue. Graphene sheets are promising local heating agents due to their strong absorbance of near‐infrared radiation. Experimental studies have been conducted to study the heating effect of graphene in photothermal therapy, yet few efforts have been devoted to the quantitative understanding of energy conversion and transport in such...
Sun Nov 03, 2019 16:49
Abstract Heart Failure (HF) refers to the heart's inability to pump sufficient blood to maintain the body's needs, which has a very serious impact on human health. In recent years, the prevalence of heart failure has remained high. This paper proposes a multi‐view ensemble learning algorithm based on empirical kernel mapping called MVE‐EK, which predicts the mortality of patient through hospital records. Multi‐view ensemble learning can take advantage of the consistency and complementarity of different...
Sun Nov 03, 2019 16:13
Δεν είμαστε φτιαγμένοι για να αισθάνονται ασφαλείς όλη την ώρα, αλλά ίσως μια μέρα θα μπορούσαμε να γίνουμε. Μια μελέτη που ερεύνησε τη νευρολογική βάση του άγχους στον εγκέφαλο έχει εντοπίσει τα «κύτταρα του άγχους». Βρίσκονται στον ιππόκαμπο και ρυθμίζουν την ανήσυχη συμπεριφορά μας αλλά μπορούν να ελεγχθούν από μια δέσμη φωτός. Τα ευρήματα, που μέχρι στιγμής έχουν προέλθει από πειράματα σε εργαστηριακά ποντίκια, θα μπορούσαν να προσφέρουν μια ακτίνα ελπίδας για τα εκατομμύρια των ανθρώπων παγκοσμίως...
10:18
Abstract Pathogenic GFM1 variants have been linked to neurological phenotypes with or without liver involvement, but only a few cases have been reported in the literature. Here, we report clinical, biochemical and neuroimaging findings from nine unrelated children carrying GFM1 variants, 10 of which were not previously reported. All patients presented with neurological involvement—mainly axial hypotonia and dystonia during the neonatal period—with five diagnosed with West syndrome; two children...
Sun Nov 03, 2019 18:05
ABSTRACT Precise identification of causative variants from whole‐genome sequencing data, including both coding and non‐coding variants, is challenging. The CAGI5 SickKids clinical genome challenge provided an opportunity to assess our ability to extract such information. Participants in the challenge were required to match each of 24 whole‐genome sequences to the correct phenotypic profile and to identify the disease class of each genome. These are all rare disease cases that have resisted genetic...
Sun Nov 03, 2019 18:04
Abstract Whole exome sequencing (WES) widely used to detect genetic mutations that cause Mendelian diseases, and has been successfully applied in combination with preimplantation genetic diagnosis (PGD) to avoid the transmission of genetic defects. We investigated 40 non‐consanguineous families with unexplained, recurrent fetal malformations (two or more malformed fetuses) from May 2016 to December 2018. Using Trio‐WES, we identified 32 disease‐associated variants in 40 families (80% positive rate),...
Sun Nov 03, 2019 18:03
No abstract available
Fri Nov 01, 2019 02:00
Objective: To compare early operative treatment with nonoperative treatment of fragility fractures of the pelvis regarding mortality and functional outcome. Design: Retrospective. Setting: Two trauma centers. Patients and Methods: Two hundred thirty consecutive patients 60 years of age or older with an isolated low-energy fracture of the pelvis and with a follow-up of at least 24 months. In center 1, treatment consisted of a nonoperative attempt and early operative fixation if mobilization...
Fri Nov 01, 2019 02:00
In the present study, we analyzed RNA‐seq data of paired left and right atrial appendages from five patients with atrial fibrillation and other five patients without AF and identified a set of functional long noncoding RNAs which may contribute to AF initiation. Abstract Long noncoding RNAs (lncRNAs) are an emerging class of RNA species that could participate in some critical pathways and disease pathogenesis. However, the underlying molecular mechanism of lncRNAs in atrial fibrillation (AF) is...
Sun Nov 03, 2019 20:00
1. Both PCED1B‐AS1 and PCED1B are high‐expressed in glioma. 2. PCED1B‐AS1 or PCED1B silence inhibits the proliferation and enhances the apoptosis. Abstract Glioma with poor prognosis is accepted as a lethal, malignant intracranial tumor among central nervous system diseases. It has been frequently exhibited that long noncoding RNAs (lncRNAs) exert indispensable functions in glioma through regulating gene expression through various molecular mechanisms. To unveil a novel lncRNA functioning...
Sun Nov 03, 2019 19:59
The circ‐Foxo3/miR‐23a/PTEN pathway is possibly a critical factor in inhibiting esophageal squamous cell cancer (ESCC) progression. This may provide a promising target for treating ESCC. Abstract Circ‐Foxo3 is a circRNA encoded by the human FOXO3 gene and works as a sponge for potential microRNAs (miRNAs) to regulate cancer progression. However, the role of circ‐Foxo3 in esophageal squamous cell cancer (ESCC) is not clear. In this study, circ‐Foxo3 was lowly expressed in cell lines and ESCC tissues....
Sun Nov 03, 2019 19:59
We found that long noncoding RNA (lncRNA)‐XIST knockdown inhibits cell viability, migration, invasion, resistance to apoptosis, and glucose metabolism of glioblastoma cells. LncRNA‐XIST functions as a competing endogenous RNA (ceRNA) of microRNA‐126 (miR‐126) and then regulates the IRS1/PI3K/Akt pathway in glioblastoma cells. In vivo results were reproduced in in vivo studies, which demonstrated lncRNA‐XIST knockdown reduces the tumorigenicity of glioblastoma cells.The study provides a rationale...
Sun Nov 03, 2019 19:58
In this review, we summarize recent literature concerning innate lymphoid cells subsets and their function in cancer and also the most prevalent cancers associated with ILCs are discussed in detail, in addition to new insights into therapeutic approaches. Abstract Innate lymphoid cells (ILCs) as key players in innate immunity have been shown to be significantly associated with inflammation, lymphoid neogenesis, tissue remodeling, mucosal immunity and lately have been considered a remarkable nominee...
Sun Nov 03, 2019 19:58
LINC00467 is upregulated and related to poor prognosis in lung adenocarcinoma (LAD). Knockdown of LINC00467 inhibits proliferation, promoted apoptosis, and inhibited stemness of LAD cells. Abstract Lung adenocarcinoma (LAD), as one of the most common types of lung tumors, is lethal and malignant. Long noncoding RNAs (lncRNAs) play important roles in various cancers according to many previous studies. LINC00467 was proposed to be a tumor promoter. Despite the validated promotive effect of LINC00467...
Sun Nov 03, 2019 19:57
The current study confirmed the osteogenic characteristics of synovial mesenchymal stem cells induced by bone morphogenetic protein 9 in vitro and vivo and explored the underlying mechanism behind it. Abstract Synovial mesenchymal stem cells (SMSCs) with high proliferation and multi differentiation ability, and low immunogenicity have attracted research attention for their potential application in tissue engineering. Once their ability of osteogenesis is strengthened, it will be of practical value...
Sun Nov 03, 2019 19:56
UCA1 promoted proliferation and invasion and metastasis of glioma by regulating the inhibition of miR‐135a, suggesting that UCA1 may be a potential oncogene of glioma. It would provide experimental basis for clinical prognosis judgment and further targeted interventional therapy of this tumor. Abstract The dysregulation of long noncoding (lncRNA) UCA1 may play an important role in tumor progression. However, the function in gliomas is unclear. Therefore, this experiment was designed to explore...
Sun Nov 03, 2019 19:56
Interleukin 1β (IL1β) plays an essential role in periodontal disease. IL1β inhibited the differentiation of cementoblasts via activating miR‐325‐3p expression. Applying miR‐325‐3p inhibitors provided therapy in cementum loss caused by periodontitis. Abstract Cementum regeneration is considered the gold standard for the treatment of periodontitis. As one of the most important primary proinflammatory cytokines, interleukin 1β (IL1β) plays an essential role during the early stage of periodontitis...
Sun Nov 03, 2019 19:56
AGEs induces apoptosis and autophagy via reactive oxygen species in human periodontal ligament cells
Advanced glycation end products induce an autophagic response through the ERK signaling pathway mediated by reactive oxygen species, thereby inhibiting apoptosis. Abstract The apoptosis of human periodontal ligament cells (HPDLCs) may be an important factor of the negative effect of advanced glycation end products (AGEs) on the periodontal tissue of diabetic patients. However, the pathways or potential effects of apoptosis in AGEs‐treated HPDLCs have not been fully elucidated. Autophagy is closely...
Sun Nov 03, 2019 19:55
Abstract Hepatitis B virus (HBV) infection is the most common cause of hepatocellular carcinoma (HCC) worldwide, wherein the expression of alpha‐fetoprotein (AFP) is reactivated to promote tumorgenesis. Hepatitis B virus X protein (HBx) protein encoded by the HBV virus X gene has been considered to be oncogenic and implicated in hepatocarcinogenesis. However, the relationship between HBx and abnormal AFP expression in HCC is yet to be fully understood. To explore the potential regulation of HBx...
Sun Nov 03, 2019 19:55
Five‐lncRNA signature can be used to independently predict the survival of patients with colon cancer. The patients in group with high risk showed an obviously shorter survival time compared with patients in group with low risk (log‐rank test P value < .0001) with area under the curve (AUC) of 0.75. Abstract Dysregulation of long noncoding RNAs (lncRNAs) has been found in a large number of human cancers, including colon cancer. Therefore, the implementation of potential lncRNAs biomarkers...
Sun Nov 03, 2019 19:53
Epidermal cells were sorted with differential expression of α6 and β1 integrins to explore epidermal heterogeneity. Using bulk‐cell RNA‐seq, nine cell populations sorted were identified as the main cell types in epidermis. Using single‐cell RNA‐seq, 576 single cells were sorted from re‐epithelialized wounds and identified 13 epidermal cell populations. Abstract Skin epidermis is a constantly renewing epithelium that is composed of various cell types and provides an ideal model system for tissue...
Sun Nov 03, 2019 19:53
In this paper, based on integrated bioinformatics analysis, we identified a novel stemness‐related long noncoding RNA (lncRNA) termed lncRNA‐cCSC1, which is significantly differential expression in colorectal cancer (CRC). In addition, we found it is upregulated in both CRC and colorectal cancer stem cells (CRCSCs) and could induce a poor prognosis. In a further step, the biological functions of lncRNA‐cCSC1 were assessed through silencing or upregulating the lncRNA expression in vitro and vivo....
Sun Nov 03, 2019 19:53
We report that F‐box protein 31 (FBXO31) is upregulated in carbon tetrachloride (CCl4) induced liver fibrosis and in activated hepatic stellate cells, induced by transforming growth factor‐β (TGF‐β). The upregulation of FBXO31 enhances activation of HSCs. FBXO31 decreased accumulation of Smad7 in HSCs by promoting ubiquitination of Smad7 Abstract Liver fibrosis is a critical pathological process in the early stage of many liver diseases, including hepatic cirrhosis and liver cancer. However,...
Sun Nov 03, 2019 19:52
In addition, hepatitis B virus (HBV) could induce CAPN2 expression, and the interference of CAPN2 could inhibit the expression of hepatic fibrosis markers, including a‐SAM, COL3A1, COL1A1, and MAPK1. CAPN2 is regarded as a biomarker of hepatic fibrosis induced by HBV. Abstract This study is aimed to investigate whether calpain 2 (CAPN2) serves as an indicator of the hepatitis B virus (HBV) to induce hepatic fibrosis. Differentially‐expressed genes (DEGs) in HBV‐induced hepatic fibrosis and normal...
Sun Nov 03, 2019 19:52
miR‐181b‐5p could suppress prostate cancer cell proliferation, migration, and invasion via targeting OSM to affect Raw264.7 preosteoclast cell osteoclastic differentiation. Abstract The activation of osteoblasts is significantly correlated to prostate tumor bone metastasis and bone loss. Oncostatin M (OSM) could promote breast cancer metastasis to bone. However, its role and mechanism in prostate cancer bone metastasis remain unclear. MicroRNAs (miRNAs) could play important roles in cancers via...
Sun Nov 03, 2019 19:52
The [MnL(CH3OH)2] complex shows a significant antioxidant activity against the 2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulfonic acid) and 2,2‐diphenyl‐1‐picrylhydrazyl‐hydrate radicals. The complex has a significant dose‐ and time‐dependent cytotoxic effects. The complex induces an apoptotic death in both of the MCF7 and HepG2 cancer cells. Abstract Herein, a Mn(II) complex of the N,N′‐dipyridoxyl(1,4‐butanediamine) (═H2L) Schiff base has been newly synthesized. The synthesized complex was characterized...
Sun Nov 03, 2019 19:51
1. Circ_0074027 is upregulated and correlates with poor prognosis for the patients with NSCLC. 2. Circ_0074027 could be a competing endogenous RNA to competitively sponge miR‐185‐3p to upregulate bromodomain‐containing protein 4 (BRD4) and MAPK‐activating death domain‐containing protein (MADD) expression. 3. Circ_0074027 executes oncogenic properties by regulating BRD4 and MADD levels. Abstract Circular RNAs play an imperative role in cancer development and metastasis by regulating oncogenic and...
Sun Nov 03, 2019 19:50
A classifier of cervical cancer was constructed based on the methylation spectrum of The Cancer Genome Atlas to identify prognostic specific methylation. Help identify new cervical cancer markers or molecular subtypes. To provide guidance for clinicians in the diagnosis and prognosis of different epigenetic subtypes. Abstract Cervical cancer is one of the leading female health‐killers among all types of malignancies globally. Human papillomavirus infection combined with genetic and epigenetic alterations...
Sun Nov 03, 2019 19:50
1.5 mM NEFAs or BHBA impaired hepatocytes fatty acid oxidation, synthesis, and transport. Choline and methionine promote lipid oxidation and transport in damaged hepatocytes. Abstract High concentrations of nonesterified fatty acids (NEFAs) and β‐hydroxybutyric acid (BHBA) induce lipid peroxidation, resulting in liver damage. Choline and methionine (Met) can promote energy balance and benefit liver health in transition dairy cows; however, the regulating mechanism remains unclear. In the present...
Sun Nov 03, 2019 19:50
TMPO‐AS1 expression is upregulated in osteosarcoma (OS) tissues and cells, and TMPO‐AS1 knockdown inhibits cell proliferation whereas induces cell apoptosis. TMPO‐AS1 serves as a sponge of miR‐199a‐5p and miR‐199a‐5p is a tumor‐suppressor in OS. Abstract Osteosarcoma (OS) is a common kind of aggressive tumor in bone which was mostly identified in children and adolescents with extremely high risk of death. Accumulating research works have displayed that long noncoding RNAs (lncRNAs) exert an essential...
Sun Nov 03, 2019 19:50
Silencing of cZNF292 abated growth, migration, and invasion of Eca‐109 cells by upregulating miR‐206, which subsequently modulated AMP‐activated protein kinase (AMPK) and PI3K/AKT signal pathways. Abstract Circular RNA (circRNA) cZNF292 has been previously revealed as a circular oncogenic RNA. This study attempted to illustrate the functions of cZNF292 in human esophageal carcinoma Eca‐109 cells. Eca‐109 cells were transfected with the short hairpin RNA specific against cZNF292 (sh‐cZNF292) and/or...
Sun Nov 03, 2019 19:50
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