- Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in AstrocytesHere we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.
- In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR ToolboxQuantitative structure-activity relationship (QSAR) modeling is a representative bioinformatics-assisted method in toxicological screening. This protocol demonstrates how to computationally assess the risks of endocrine disruptors (EDs) in aquatic environments. Utilizing the OECD QSAR Toolbox, the protocol implements an in silico assay for analyzing toxicity of EDs in fish.
- Stochastic Noise Application for the Assessment of Medial Vestibular Nucleus Neuron Sensitivity In VitroGalvanic vestibular stimulation in humans exhibits improvements in vestibular function. However, it is unknown how these effects occur. Here, we describe how to apply sinusoidal and stochastic electrical noise and evaluate appropriate stimulus amplitudes in individual medial vestibular nucleus neurons in the C57BL/6 mouse.
- Corrigendum to “Hypothesis: Metabolic targeting of 5-aminolevulinate synthase by tryptophan and inhibitors of heme utilisation by tryptophan 2,3-dioxygenase as potential therapies of acute hepatic porphyrias” [Med. Hypotheses 131 (2019) 109314]Publication date: November 2019Source: Medical Hypotheses, Volume 132Author(s): A.A.-B. Badawy
- Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse MaturationThis protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. The procedure facilitates imaging-based assays of neurites, synapses, and late-expressing neuronal markers in a format compatible with light microscopy and high-content screening.
- Epidemiology and Treatment of Multidrug-Resistant and Extensively Drug-Resistant Pseudomonas aeruginosa Infections [Reviews]In recent years, the worldwide spread of the so-called high-risk clones of multidrug-resistant or extensively drug-resistant (MDR/XDR) Pseudomonas aeruginosa has become a public health threat. This article reviews their mechanisms of resistance, epidemiology, and clinical impact and current and upcoming therapeutic options. In vitro and in vivo treatment studies and pharmacokinetic and pharmacodynamic (PK/PD) models are discussed. Polymyxins are
- The shift from typical Western European Late Acheulian to microproduction in unit ‘D’ of the late Middle Pleistocene deposits of the Caune de l’Arago (Pyrénées-Orientales, France)Publication date: October 2019Source: Journal of Human Evolution, Volume 135Author(s): Deborah Barsky, Anne Marie Moigne, Véronique PoisAbstractStratigraphic Unit D levels of the Caune de l’Arago (Pyrénées-Orientales, France), situated in the upper part of the depositional sequence of Ensemble Stratigraphique III (ES III), has yielded a rich Acheulian archeopaleontological record dated to the Middle Pleistocene. The site's infill, dated from 690
- How to handle hypertriglyceridaemia in acute pancreatitis ‐ Still a vote for conservatives?"Abstract The observation of opalescent blood when drawing samples from a patient with suspect acute pancreatitis is not uncommon in the emergency room, and the confirmation of massive hypertriglyceridaemia by the laboratory generally initiates the surgeon on duty to take a rapid contact with the internist colleague. Hypertriglyceridaemia is present in about one of ten patients with acute pancreatitis, and can frequently be linked to excessive al
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