Colorectal cancer (CRC) remains one of the most prevalent gastrointestinal tract malignancies in China. Accumulating evidence indicates that long intergenic non-coding RNAs (lincRNAs) play a crucial role in predicting survival of patients with CRC. However, the clinical significance of novel lincRNA, LINC00957 in CRC remains to be elucidated. Therefore, the present study aimed to investigate the expression profile and clinical significance of LINC00957 expression in CRC tumor tissues. Furthermore,...
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Objectives: CD44, a transmembrane glycoprotein, is involved in the generation of a stem cell niche and maintaining stem cell quiescence. The aim of this study was to evaluate its contribution to ovarian cancer prognosis and progression, as well as explore the possible mechanisms. Materials and Methods: The expression of CD44 in tissue microarray of 90 ovarian cancer patients was detected by immunohistochemistry. Kaplan-Meier method and Cox proportional hazard model were used to evaluate the factors...
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The addition of cetuximab to platinum-based chemotherapy (cisplatin or carboplatin plus 5-fluorouracil [5-FU]), followed by maintenance cetuximab until disease progression (EXTREME), resulted in the first regimen to yield significantly improved survival outcomes in the first-line treatment of patients with recurrent and/or metastatic squamous cell carcinoma of the head and neck (R/M SCCHN) in over 30 years. Currently, the EXTREME regimen is a guideline-recommended treatment in the first-line R/M...
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Purpose: To investigate the impact of radiation treatment quality assurance (RTQA) on treatment outcomes in a phase III trial for advanced head and neck cancer. Materials and methods: A total of 767 patients from NRG/RTOG 0522 were included in this study. The contours of target volume (TV) and organ at risk (OAR), and dose-volume coverage of targets were reviewed and scored (per-protocol, variation-acceptable and deviation-unacceptable) according to the protocol. We performed log-rank tests...
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Epigenetic information is characterized by its stable transmission during mitotic cell divisions and plasticity during development and differentiation. This duality contrasts to genetic information, which is essentially static and identical in every cell in an organism except immunoglobulin gene rearrangements in lymphocytes and mutations in cancer cells. Allele-specific analysis of gene expression and epigenetic modifications provides a unique approach to studying epigenetic regulation in normal...
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Colorectal cancer is the third leading cause of cancer-related death worldwide. We have previously shown that Origanum majorana ethanolic extract (OME) promoted mitotic arrest, induced apoptosis and inhibited migration, metastasis and tumor growth of triple negative breast cancer. Here, we investigated the potential anticancer effect and the molecular mechanism of Origanum majoran ethanolic extract against human colorectal cancer cells. Our results showed that OME exhibited strong anti-proliferative...
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The aim of this study was to evaluate the effect of bleaching agents containing different concentrations of hydrogen peroxide (HP) on color-change and on enamel-surface in bovine teeth. Furthermore the influence on cell viability and proliferation was investigated. 240 teeth were randomly assigned into four groups, (home bleaching ≤ 6%, in-office bleaching ≤ 6%, in-office bleaching > 6% HP and control group). Bleaching was performed after artificial staining and the bleached index BI as well...
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Vitellogenins are a family of yolk proteins that are by far the most abundant among oviparous animals. In the model nematode Caenorhabditis elegans, the 6 vitellogenins are among the most highly expressed genes in the adult hermaphrodite intestine, which produces copious yolk to provision their eggs. In this article we review what is known about the vitellogenin genes and proteins in C. elegans, in comparison with vitellogenins in other taxa. We argue that the primary purpose of abundant vitellogenesis...
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Skeletal muscle is composed of a large and heterogenous assortment of cell populations that interact with each other to maintain muscle homeostasis and orchestrate regeneration. Although Satellite Cells (SCs) - which are muscle stem cells - are the protagonists of functional muscle repair following damage, several other cells such as inflammatory, vascular and mesenchymal cells coordinate muscle regeneration in a finely tuned process. Fibro-Adipogenic Progenitors (FAPs) are a muscle interstitial...
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Lipophagy degrades lipid droplets (LDs) through the lysosomal degradative pathway, thus plays important roles in regulating lipid metabolism in mammals. However, information on the existence and functions of lipophagy in fish lipid metabolism is still limited. In the present study, we confirmed the existence of lipophagy by observing the structures of LDs sequestered in autophagic vacuoles in the zebrafish liver cell line (ZFL) via electronic microscopy. Moreover, starved cells increased the mRNA...
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Barnacles form a globally ubiquitous group of sessile crustaceans that are particularly common in the coastal intertidal. Several barnacle species are described as highly euryhaline and a few species even have the ability to colonize estuarine and brackish habitats below 5 PSU. However, the physiological and/or morphological adaptations that allow barnacles to live at low salinities are poorly understood and current knowledge is largely based on classical eco-physiological studies offering limited...
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The cephalopods (Mollusca: Cephalopoda) are an exceptional class among the invertebrates, characterised by the advanced development of their conditional learning abilities, long-term memories, capacity for rapid colour change and extremely adaptable hydrostatic skeletons. These traits enable cephalopods to occupy diverse marine ecological niches, become successful predators, employ sophisticated predator avoidance behaviours and have complex intraspecific interactions. Where studied, observations...
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The current study assessed the effect of dietary canthaxanthin and lycopene supplementation at different concentrations on growth performance and antioxidant status in yellow perch (Perca flavescens). In this regard, fish with initial weight (32 ± 1.0 g) were divided into five groups in triplicate, and fed on carotenoid-free diet (control), canthaxanthin (CTX) (50 and 100 mg/kg diet), and lycopene (200 and 400 mg/kg diet) for 60 days. Growth parameters and antioxidant enzymes were evaluated after...
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Although robot-assisted training is present in various fields such as sports engineering and rehabilitation, provision of training strategies that optimally support individual motor learning remains as a challenge. Literature has shown that guidance strategies are useful for beginners, while skilled trainees should benefit from challenging conditions. The Challenge Point Theory also supports this in a way that learning is dependent on the available information, which serves as a challenge to the...
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Human-robot cooperation is increasingly demanded in industrial applications. Many tasks require the robot to enhance the capabilities of humans. In this scenario, safety also plays an important role in avoiding any accident involving humans, robots and the environment. With this aim, the paper proposes a cooperative fuzzy-impedance control with embedded safety rules to assist human operators in heavy industrial applications while manipulating unknown weight parts. The proposed methodology is composed...
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Publication date: November 2019Source: Journal of Functional Foods, Volume 62Author(s): Xuyun Liu, Ke Cao, Weiqiang Lv, Jing Liu, Jing Gao, Yan Wang, Chuan Qin, Jianshu Liu, Weijin Zang, Jiankang LiuAbstractIncreasing evidence demonstrates that hyperlipidemia plays a causal role in the development of cardiovascular diseases (CVDs) due to endothelial dysfunction. Houttuynia cordata aqueous extract (HAE), using as both medicine and food, whose effect and mechanism were investigated in endothelial dysfunction....
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Publication date: October 2019Source: Journal of Functional Foods, Volume 61Author(s): Marina Raju, Yogesh A. Kulkarni, Sarika WairkarAbstractCurrently, phytoconstituents have been emerged as a scientific interest in discovering novel therapeutics against numerous diseases. Berberine is a bioactive alkaloid, historically reported for pharmacological actions such as diuretic, antidiarrheal, antimicrobial and antiprotozoal etc. Scientific evidences exhibit its versatile therapeutic uses against several...
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Future Oncology, Ahead of Print.
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Future Oncology, Ahead of Print.
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Future Oncology, Ahead of Print.
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Future Oncology, Ahead of Print.
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Related ArticlesSuggested parameters to setup Y chromosome microsatellites markers as prostate cancer genetic risk indicator. Future Oncol. 2019 Aug;15(24):2791-2794 Authors: Abumsimir B, Ennaji MM PMID: 31429608 [PubMed - in process]
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Related ArticlesComprehensive analysis of non-small-cell lung cancer microarray datasets identifies several prognostic biomarkers. Future Oncol. 2019 Aug 20;: Authors: Qin X, Chen R, Xiong R, Tan Z, Gao S, Lin C, Huo T Abstract Aim: To find accurate and effective biomarkers for diagnosis of non-small-cell lung cancer (NSCLC) patients. Materials & methods: We downloaded microarray datasets GSE19188, GSE33532, GSE101929 and GSE102286 from the database...
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Related ArticlesIs there a role for carbon therapy in the treatment of gynecological carcinomas? A Systematic Review. Future Oncol. 2019 Aug 20;: Authors: Wang L, Wang X, Zhang Q, Ran J, Geng Y, Feng S, Li C, Zhao X Abstract This Systematic Review summarizes the literatures of clinical trials on the efficacy and safety of carbon ion therapy for gynecological carcinomas. The protocol is detailed in the online PROSPERO database, registration no. CRD42019121424,...
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Related ArticlesPrognostic impact of K-RAS mutational status and primary tumor location in patients undergoing resection for colorectal cancer liver metastases: an update. Future Oncol. 2019 Aug 20;: Authors: O Connor JM, Sanchez Loria F, Ardiles V, Grondona J, Sanchez P, Andriani O, Fauda M, Brancato F, Huertas E, Alvarez F, de Santibañes E Abstract Aim: To determine the impact of KRAS mutation status on survival in patients undergoing surgery for colorectal...
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Related ArticlesShould stereotactic body radiotherapy doses be adjusted according to tumor size in early-stage non-small-cell lung cancer? A Systematic Review and meta-analysis. Future Oncol. 2019 Aug 20;: Authors: Luo H, Cui Y, Song H, Mao R, Gao Q, Ge H Abstract Aim: Treatment schedules of stereotactic body radiotherapy (SBRT) for patients with early-stage non-small-cell lung cancer (NSCLC) are varied. The aim of this study was to clarify the optimal...
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Related ArticlesEMERALD: Phase III trial of elacestrant (RAD1901) vs endocrine therapy for previously treated ER+ advanced breast cancer. Future Oncol. 2019 Aug 20;: Authors: Bardia A, Aftimos P, Bihani T, Anderson-Villaluz AT, Jung J, Conlan MG, Kaklamani VG Abstract Elacestrant is a novel, nonsteroidal, orally bioavailable selective estrogen receptor degrader (SERD) that has demonstrated activity in patients with estrogen receptor (ER)-positive/HER2-negative...
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Endoscopic submucosal dissection (ESD) is widely used as a minimally invasive treatment for large esophageal cancers, but prolonged procedure duration and life-threatening adverse events remain matters of concern. We aimed to determine whether traction-assisted ESD (TA-ESD) is superior to conventional ESD in terms of technical outcomes.
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We developed a computer-assisted diagnostic (CAD) system for real-time automated diagnosis of precancerous lesions and early esophageal squamous cell carcinoma (ESCC) to assist the diagnosis of esophageal cancer.
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Genes & Immunity, Published online: 22 August 2019; doi:10.1038/s41435-019-0083-yThe type 1 diabetes candidate gene Dexi does not affect disease risk in the nonobese diabetic mouse model
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Genes & Immunity, Published online: 22 August 2019; doi:10.1038/s41435-019-0084-xMicroRNA-146a negatively regulates IL-33 in activated group 2 innate lymphoid cells by inhibiting IRAK1 and TRAF6
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Genes & Immunity, Published online: 22 August 2019; doi:10.1038/s41435-019-0083-yThe type 1 diabetes candidate gene Dexi does not affect disease risk in the nonobese diabetic mouse model
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Genes & Immunity, Published online: 22 August 2019; doi:10.1038/s41435-019-0084-xMicroRNA-146a negatively regulates IL-33 in activated group 2 innate lymphoid cells by inhibiting IRAK1 and TRAF6
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GSE136105 Gene expression profiling in lung and breast cancer cells treated by Bloom-specific siRNAs
Contributors : Bin Shan ; Simon AlsagerSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensA549 and MDA-MB-231 cells were transfected with the Bloom-specific siRNA. Total RNA was extracted using Trizol at 48 hours after transfection. RNA-SEQ was carried out to profile the gene expression in both culture conditions.
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Contributors : Kasiani C Myers ; Nathan SalomonisSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo evaluate the transcriptome and splicing repertoire of bulk CD34+ sorted bone marrow progenitors we performed RNA-Seq. These data highlight an AML prognostic splicing signature that is present in healthy donors at equivalent frequencies to that found in AML bone marrow.
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Contributors : Lokesh A Kalekar ; Jarish N CohenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusComparison of regulatory T cell transcriptional signatures in adult mouse skin and lung.
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Contributors : Ryan D Chow ; Sidi ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCD8+ cytotoxic T cells play essential roles in anti-tumor immune responses. Here, we performed in vivo screens in CD8+ T cells and identified regulators of tumor infiltration and killing, which are directly relevant to cancer immunotherapy. Unlike in vitro screens, the in vivo screen robustly re-identified canonical immunotherapy targets such as PD-1 and Tim-3, along with...
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Contributor : Kim GreenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis dataset allows for the exploration of gene expression of microdissected striatum from 11 week old wild-type and the R6/2 mouse model of Huntington's disease, in the presence or absence of microglia. Microglia were depleted via dietary administration of the CSF1R inhibitor PLX3397 from 6 to 11 weeks of age.
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Contributors : Lawrence W Chamley ; Jia WeiSeries Type : Expression profiling by arrayOrganism : Homo sapiensThe human placenta is covered by a single multinucleated fetal cell, the syncytiotrophoblast, which is bathed in maternal blood. During all pregnancies, membrane enclosed extracellular vesicles derived from the syncytiotrophoblast are extruded into the maternal blood.The large size of these extracellular vesicles (diameter larger than 10 µm) is referred to as trophoblast debris in this study....
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Contributors : Alison Hirukawa ; William J MullerSeries Type : Expression profiling by arrayOrganism : Mus musculusMonoclonal antibodies (mAbs) targeting the oncogenic receptor tyrosine kinase ERBB2/HER2, such as Trastuzumab, are the standard of care therapy for breast cancers driven by ERBB2 overexpression and activation. However, a substantial proportion of patients exhibits de novo resistance. Here, by comparing matched Trastuzumab-naïve and post-treatment patient samples from a neoadjuvant trial,...
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Contributors : Sifan Chen ; Sudha BiddingerSeries Type : Expression profiling by arrayOrganism : Rattus norvegicusTMAO is gut microbiota dependent metabolite catalyzed by monooxygenase FMO3 from TMA. TMAO is positively assocaited with different metabolic diseases such as diabetes, chronic kidney disease, and atherosclerosis.We used microarray to analyze the genes regulated by TMAO treatment in primary rat hepatocytes to further understand the mechanistic view of TMAO action.
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Contributors : Kellen J Cavagnero ; Jana H Badrani ; Alice Wang ; Gregory Seumois ; Taylor A DohertySeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusBackground: Alternaria exposure is associated with severe asthma in humans. Alternaria exposure in mice potently activates group 2 innate lymphoid cells (ILC2s) via the IL-33/ST2 axis and causes ILC2s to robustly secrete type 2 cytokines. Objective: Our aim was to determine whether conventionally used ILC2 markers,...
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Contributors : Jie Gao ; Ruiqiong Liu ; Yan WangSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensWe report a series of ChIP-Seq results in HeLa cells. Briefly, we performed ChIP-Seq experiments with antibodies specific for Snail, MTA1, and PRMT5 in HeLa cells. We found that Snail, MTA1, and PRMT5 had similar binding motifs. This study gave us a new understanding of the role of Snail in chromatin status and gene transcription.
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Contributors : Pawan Kumar ; Peng CheeSeries Type : Expression profiling by high throughput sequencingOrganism : Gossypium hirsutumWe applied the comparative transcriptomic approach to compare expression profiles of genes between RKN susceptible and resistance genotypes at an early stage of RKN development that coincides with the establishment of a feeding site and at the late stage of RKN development that coincides with RKN egg production. Sequencing of cDNA libraries produced over 315 million...
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Contributors : Markus H Drag ; Lisette J Kogelman ; Hanne Maribo ; Lene Meinert ; Preben D Thomsen ; Haja N KadarmideenSeries Type : Genome variation profiling by SNP array ; SNP genotyping by SNP arrayOrganism : Sus scrofaBoar taint is an offensive odour and flavour from developed in a proportion of non-castrated male pigs (boars). Surgical castration is an effective solution but is under debate due to animal welfare concerns. Gene-based selection for low boar taint has been proposed as concentrations...
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Contributors : Josh Knowles. ; Wenyu Zhou.Series Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA-seq mRNA transcriptome profiling of human induced pluripotent stem (hiPSCs) cells to identify the effect of insulin on regulation of hiPSC transcriptome
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Contributors : Natsuki Shinoda ; Masayuki MiuraSeries Type : Expression profiling by arrayOrganism : Drosophila melanogasterCaspase is best known as an enzyme involved in programmed cell death that is conserved in multicellular organisms. In addition to its role in cell death, caspase is emerging as an indispensable enzyme for a wide range of cellular functions, which have recently been termed as Caspase-Dependent non-lethal cellular Processes. We carefully examined the involvement of cell death...
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Contributors : Maria L Rodgers ; Robert J GriffittSeries Type : Expression profiling by high throughput sequencingOrganism : Sciaenops ocellatusThe objective of this study was to determine how transcriptomic responses in the spleen and liver are altered in juvenile Sciaenops ocellatus exposed to oil, a known fish pathogen (Vibrio anguillarum), or both.
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Contributors : Guilherme Pugliesi ; Sónia C da Silva AndradeSeries Type : Expression profiling by high throughput sequencingOrganism : Bos indicusWe aimed with this study through RNAseq, found diferentially expressend genes (DEGs) that can be used as a new pregnancy markers in peripheral blood mononuclear cells (PBMC) on day 18 post artificial insemination; and to assess the mRNA profile by RT-qPCR of new pregnancy markers in PBMC and peripheral blood polymorphonuclear cells (PMN) at early pregnancyMethods:...
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Contributors : Teruhiko Makino ; Yoshiaki TabuchiSeries Type : Expression profiling by arrayOrganism : Homo sapiensTCHHL1 (trichohyalin-like 1) protein is a novel member of the fused-type S100 protein gene family. Previous findings suggest that TCHHL1 may play a role in the proliferation of keratinocytes.In the present study, to clarify a role of TCHHL1 in the proliferation of keratinocytes, we performed global-scale gene expression analysis in TCHHL1-Knockdown keratinocytes using an Affymetrix...
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Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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Contributors : Joaquín Sanz ; Jenny Tung ; Luis BarreiroSeries Type : Expression profiling by high throughput sequencingOrganism : Macaca mulattaSocial experiences are an important predictor of disease susceptibility and survival in humans and other social mammals. Chronic social stress is thought to generate a pro-inflammatory state characterized by elevated antibacterial defenses and reduced investment in antiviral defense. Here, we manipulated long-term social status in female rhesus macaques...
41m
Contributors : Ryan D Chow ; Sidi ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCD8+ cytotoxic T cells play essential roles in anti-tumor immune responses. Here, we performed in vivo screens in CD8+ T cells and identified regulators of tumor infiltration and killing, which are directly relevant to cancer immunotherapy. Unlike in vitro screens, the in vivo screen robustly re-identified canonical immunotherapy targets such as PD-1 and Tim-3, along with...
41m
Contributors : Wooje Lee ; Qizhi GongSeries Type : Methylation profiling by high throughput sequencingOrganism : Homo sapiensMeCP2 plays a multifaceted role in gene expression regulation and chromatin organization. Interaction between MeCP2 and methylated DNA to regulate gene expression is well established. However, the widespread MeCP2 distribution suggests its additional interactions with chromatin. Here we show, by both biochemical and ChIP-seq analyses, that MeCP2 directly binds to nucleosome...
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Contributors : Nicholas Collins ; Seong-Ji Han ; Michel Enamorado ; Verena M Link ; Yasmine BelkaidSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMammals evolved in the face of fluctuating food availability. How the immune system adapts to transient nutritional stress remains poorly understood. Here, we show that memory T cells collapsed in secondary lymphoid organs in the context of dietary restriction (DR) but dramatically accumulated within the bone...
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Contributors : Wooje Lee ; Qizhi GongSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Homo sapiensMeCP2 plays a multifaceted role in gene expression regulation and chromatin organization. Interaction between MeCP2 and methylated DNA to regulate gene expression is well established. However, the widespread MeCP2 distribution suggests its additional interactions with chromatin. Here we show, by both biochemical and ChIP-seq analyses, that MeCP2 directly binds...
41m
Contributors : Chris Martyniuk ; Chris SoudersSeries Type : Expression profiling by arrayOrganism : Rattus norvegicusSix samples of N27 cells were treated with 25 uM fipronil, and 6 samples were not treated (control). The treatment proceeded for 24 hours.
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Contributors : Jesus A Romo ; Hao Zhang ; Cai Hong ; Yufeng Wang ; Jose L Lopez-RibotSeries Type : Expression profiling by high throughput sequencingOrganism : Candida albicansThe opportunistic pathogenic fungus Candida albicans can cause devastating infections in severely compromised patients. Its ability to undergo a morphogenetic transition from yeast to filamentous forms allows it to penetrate tissues and cause damage, and the expression of a number of pathogenetic mechanisms are also coordinately...
41m
Contributors : Markus H Drag ; Hanne Maribo ; Lene Meinert ; Preben D Thomsen ; Haja N KadarmideenSeries Type : Expression profiling by high throughput sequencingOrganism : Sus scrofaGenes with association to androstenone, skatole and indole concentrations and important sensoric traits related to boar taint were found by differential expression and co-expression gene network analysis.
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Contributor : Zheng ZhangSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapienscircRNA sequencing for 6 samples
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Contributors : Ki-Hoon Song ; Tulip S Nandu ; Shrikanth GadadSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensPurpose: To identify downstream signaling pathways that mediate functions of GALNT14Methods: RNAs isolated from MDA231-LM2 cells expressing shCntr or shGALNT14 and MDA231-Par cells expressing pBabe-Hygro control vector or GALNT14 expression vector were analyzed by using an Illumina HiSeq 2500Conclusions: Our study represents the first transcriptome...
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Series Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
6h
Contributors : David P Labbé ; Giorgia Zadra ; Massimo Loda ; Myles BrownSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSystemic metabolic alterations associated with increased consumption of saturated fat and obesity are linked with increased risk of prostate cancer progression and mortality but the molecular underpinnings of this association are poorly understood. Furthermore, the mechanisms by which metabolic rewiring alters the prostate cancer epigenome,...
6h
Contributors : Sungmin Hwang ; Amy K Schmid ; Julie A Maupin-FurlowSeries Type : Expression profiling by arrayOrganism : Haloferax volcaniiIdentification of global gene expression profiling when Haloferax volcanii H26 strain is grown in minimal medium supplemented with two nitrogen sources, 10 mM L-alanine and 10 mM ammonium chloride.
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Contributors : David P Labbé ; Giorgia Zadra ; Massimo Loda ; Myles BrownSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusSystemic metabolic alterations associated with increased consumption of saturated fat and obesity are linked with increased risk of prostate cancer progression and mortality but the molecular underpinnings of this association are poorly understood. Furthermore, the mechanisms by which metabolic rewiring alters the prostate...
6h
Contributors : Yi Zhang ; Congming LuSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Arabidopsis thalianaWe performed chloroplast ChIP-seq (cpChIP-seq) to identify the possible DNA-binding sites of mTERF5 in Arabidopsis thaliana. To this end, we generated transgenic Arabidopsis plants expressing mTERF5 carrying an HA tag under the control of the CaMV 35S promoter. Then, We used the polyclonal antibody (abcam, ab9110, lot GR304617-8 ) against HA tag which...
10h
Contributors : Tamsyn Derrick ; Esmael Habtamu ; Zerihun Tadesse ; E K Callahan ; Abebaw Worku ; Bizuayehu Gashaw ; David Macleod ; David C Mabey ; Martin J Holland ; Matthew J BurtonSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensSurgery to correct trichiasis is a key component of the WHO trachoma control strategy, however unfavourable outcomes such as eyelid contour abnormalities (ECA) following surgery are relatively common. This study aimed to understand...
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Contributors : Changfu Yao ; Tanyalak Parimon ; Peter Chen ; Barry R StrippSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSingle cell RNA-seq profiling adult mouse lung cells reveal the cell heterogeneity and mechanism during lung fibrosis process.
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Contributors : Lisa Golden ; Yuichiro Itoh ; Noriko Itoh ; Rhonda VoskuhlSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo determine the role of sex chromsomes in sex differences of autoimmune disease, we used high throughput RNA sequencing to analyze the transciptomes of autoantigen specific CD4+ T cells from XX and XY- mice using the “four core genotypes” mouse model. We found higher expression of several X genes in the XY- genotype compared to XX, suggesting...
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Contributors : Changfu Yao ; Barry StrippSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusSingle cell RNA-seq profiling adult mouse lung epithelial cells reveals the heterogeneity of mouse lung epithelium.
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Contributors : Lisa Golden ; Yuichiro Itoh ; Noriko Itoh ; Rhonda VoskuhlSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo determine the role of sex chromsomes in sex differences of autoimmune disease, we used high throughput RNA sequencing to analyze the transciptomes of autoantigen specific CD4+ T cells from XX and XY- mice using the “four core genotypes” mouse model.
10h
Contributors : Shawn J Cokus ; Fang Wang ; Alvaro SagastiSeries Type : Expression profiling by high throughput sequencingOrganism : Danio rerioWe purified (FACS) and profiled (RNA-Seq) zebrafish embryo skin cells vs. nonskin cells at three early developmental stages. At two of the stages, we further purified and profiled two distinct epithelial layers of the skin: the periderm and basal cells. Our comprehensive expression analysis provides the first reported transcriptomes of these two cell types,...
10h
Contributors : Jae H Lee ; Tiyakhon Chatnaparat ; Youfu ZhaoSeries Type : Expression profiling by high throughput sequencingOrganism : Erwinia amylovoraThis RNA-seq analysis examined the global effect of CsrA and its non-coding small RNA (ncsRNA) csrB in E. amylovora under the T3SS-inducing condition. The csrA mutant showed differential expression of more than 20% genes in the genome, which include significant down-regulation of T3SS genes and those required for cell growth and viability. On the...
10h
Contributors : Mohammad Rashidian ; M I Barrasa ; Hidde L PloeghSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusImmunotherapy using checkpoint-blocking antibodies against PD-1 has produced impressive results in a wide range of cancers. However, the response remains heterogeneous among patients. We used noninvasive immuno-positron emission tomography (PET), using 89Zr-labeled PEGylated single-domain antibody fragments (nanobodies or VHHs), to explore the dynamics...
12h
Contributors : Mohammad Rashidian ; M I Barrasa ; Hidde L PloeghSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusImmunotherapy using checkpoint-blocking antibodies against PD-1 has produced impressive results in a wide range of cancers. However, the response remains heterogeneous among patients. We used noninvasive immuno-positron emission tomography (PET), using 89Zr-labeled PEGylated single-domain antibody fragments (nanobodies or VHHs), to explore the dynamics...
12h
Series Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
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Contributors : Cibele Rocha-Resende ; Scot J Matkovich ; Douglas L MannSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusIt has been shown that acetylcholine has anti-inflammatory properties. The aim of this study is to investigate the role of acetylcholine in cardiac inflammation. We used the diphtheria toxin (DT)-cardiomyocyte ablation model (DTR model) (Lavine et al., 2014 - PNAS) to induce cardiac injury (DT, 2 days, 2.5µg/kg, IP). Beginning one week prior...
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Contributors : Hui Shi ; Ruslan Strogantsev ; Nozomi Takahashi ; Anastasiya Kazachenka ; Matthew C Lorincz ; Myriam Hemberger ; Anne C Ferguson-SmithSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusKRAB-zinc finger proteins (KZFPs) represent one of the largest families of DNA binding proteins in vertebrate genomes and appear to have evolved to silence transposable elements (TEs) including...
13h
Contributors : Ugo Szachnowski ; Sara Andus ; Dominika Foretek ; Antonin Morillon ; Maxime WerySeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Naumovozyma castelliiAntisense (as)lncRNAs are extensively degraded by the nuclear exosome and the cytoplasmic exoribonuclease Xrn1 in the budding yeast Saccharomyces cerevisiae, lacking RNA interference (RNAi). Whether the ribonuclease III Dicer affects aslncRNAs in close RNAi-capable relatives remains unknown. Using genome-wide...
13h
Contributors : Scot J Matkovich ; Wenlong Jiang ; Douglas L MannSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDying cells release Damage-Associated Molecular Patterns (DAMPs) that can initiate and perpetuate sterile inflammatory responses. However, the effect of DAMPs on macrophages biology remains unclear. We therefore studied the effect of DAMPs on primary peritoneal macrophages (PPM) in vitro. We first performed unbiased transcriptomic profiling with RNA-seq...
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Contributors : Ugo Szachnowski ; Sara Andus ; Dominika Foretek ; Antonin Morillon ; Maxime WerySeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Naumovozyma castelliiAntisense (as)lncRNAs are extensively degraded by the nuclear exosome and the cytoplasmic exoribonuclease Xrn1 in the budding yeast Saccharomyces cerevisiae, lacking RNA interference (RNAi). Whether the ribonuclease III Dicer affects aslncRNAs in close RNAi-capable relatives remains unknown. Using genome-wide...
13h
Contributors : Yufei Xiong ; Jialing YaoSeries Type : Expression profiling by high throughput sequencingOrganism : Oryza sativa Japonica GroupPurpose: Analysis of gene expression level.Methods: Retinal mRNA profiles of 7 DAP endosperm of the wild-type (WT) and NF-YC11 knockout mutant were generated by deep sequencing, in triplicate, using Illumina 2000.
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Contributors : Xianlu Zeng ; Xiaoqing HanSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPurpose: CXCR2 was transfected into 32D clone 3 cells simulating the granulocyte and macrophage progenitor cells(GMPs) to confirm the role of CXCR2 in the differentiation of GMPs to macrophage and dendritic cell progenitor cells
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Contributors : Yufei Xiong ; Jialing YaoSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Oryza sativa Japonica GroupRice NF-YC11 is a transcription factor that plays a key regulatory role in storage substance accumulation during rice grain filling. To reveal the transcription regulatory network of NF-YC11 in rice, we performed genome-wide identification of NF-YC11 targets by immunoprecipitation sequencing (ChIP-seq) analyses in the NF-YC11-overexpression...
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Contributors : Jieping Huang ; Yun MaSeries Type : Expression profiling by high throughput sequencing ; Non-coding RNA profiling by high throughput sequencingOrganism : Bubalus bubalisBackground: Background: In bovines, the development and growth of adipose involves many physiological processes and plays an extremely important role in the quality of beef, however, the regulatory mechanisms underlying the differences in meat quality are largely unknown. Therefore, a buffalo adipose transcriptome...
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Contributors : Yueh-Chwen Hsu ; Chia-Lang HsuSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPlant homeodomain finger gene 6 (PHF6) encodes a 365-amino-acid protein containing two plant homology domain fingers. Germline mutations of human PHF6 cause Börjeson–Forssman–Lehmann syndrome, a congenital neurodevelopmental disorder. Loss-of-function mutations of PHF6 are detected in patients with acute leukemia, mainly of T cell lineage and in a small proportion...
13h
Series Type : Non-coding RNA profiling by high throughput sequencingOrganism : Naumovozyma castelliiThis SuperSeries is composed of the SubSeries listed below.
13h
Contributors : Jochen Bogs ; Birgit Eisenmann ; Stefan CzemmelSeries Type : Expression profiling by high throughput sequencingOrganism : Vitis viniferaEuropean grapevine cultivars (Vitis vinifera spp.) are highly susceptible to the downy mildew pathogen Plasmopara viticola. Breeding of resistant V. vinifera cultivars is a promising strategy to reduce the impact of disease management. Most cultivars that have been bred for resistance to downy mildew, rely on resistance mediated by the Rpv3 (Resistance...
13h
Contributors : Ugo Szachnowski ; Sara Andus ; Dominika Foretek ; Antonin Morillon ; Maxime WerySeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Naumovozyma castelliiAntisense (as)lncRNAs are extensively degraded by the nuclear exosome and the cytoplasmic exoribonuclease Xrn1 in the budding yeast Saccharomyces cerevisiae, lacking RNA interference (RNAi). Whether the ribonuclease III Dicer affects aslncRNAs in close RNAi-capable relatives remains unknown. Using genome-wide...
13h
Contributors : Mathew R Bloomfield ; Maggie C LouieSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo understand how chronic cadmium exposure alters the dependency of ERα in terms of gene expression, we transiently silenced ERα using ICI, an antiestrogen that promotes the degradation of ERα. MCF7 and cadmium-adapted cells (Cd7 and Cd12) were treated with ICI to mediate the degradation of ERα, and a nonbiased global gene expression analysis was conducted using...
14h
Contributors : Jose R Hernandez ; David MonkSeries Type : Methylation profiling by arrayOrganism : Homo sapiensGestational choriocarcinoma arises from the cells of conception and is usually characterized as a fast growing, invasive and aggressive malignancy. The overall incidence is approximately 1 case per 50,000 pregnancies and aside from increasing maternal age does not appear to have any other risk factors. In contrast to most malignancies, gestational choriocarcinoma is frequently treated on...
14h
Contributors : Hanif J Khameneh ; Keith W Leong ; Andrea Mencarelli ; Maurizio Vacca ; Bezalel Mambwe ; Kurt Neo ; Alice Tay ; Francesca Zolezzi ; Michael Poidinger ; Bernett Lee ; Alessandra MortellaroSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusNaïve CD62L+ CD44- CD25- CD4+ splenic mouse T cells from WT or ASC KO cells were left untreated or treated with Anti-CD3/28 antobodies for 12hr
14h
Contributors : Blanca E Himes ; Quan Lu ; Joseph Allen ; Hae-Ryung ParkSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensElectronic cigarettes (e-cigarettes) have gained their popularity as a substitute for cigarettes or cigars. Despite the widespread use of flavoring chemicals in e-cigarettes, the health impacts of the flavoring compounds, in particular their effects on critical cellular function in the lung, remain largely unknown. The goal of this study...
14h
Contributors : Francesca Conserva ; Mariagrazia Barozzino ; Francesco Pesce ; Chiara Divella ; Annarita Oranger ; Massimo Papale ; Simona Simone ; Luigi Laviola ; Francesco Giorgino ; Anna Gallone ; Paola Pontrelli ; Loreto GesualdoSeries Type : Non-coding RNA profiling by arrayOrganism : Homo sapiensDiabetic Nephropathy (DN) is a chronic complication of diabetes and the primary cause of end stage renal disease. DN can be differentially diagnosed only through histological investigation....
14h
Contributors : Myung R Park ; Donggyu Nam ; Marcos J Araúzo-Bravo ; Jeong B KimSeries Type : Expression profiling by arrayOrganism : Mus musculusGeneration of expandable induce hepatic stem cells (iHSCs) has a potential for cell-based therapy and drug screening in liver disease. Recently many groups reported reprogramming of somatic cells into hepatocyte-like cells by direct conversion. However, the induced hepatocytes have limitations in proliferation, function and viability for cell therapy and...
14h
Contributors : Kazuki Sudo ; Ken Kato ; Juntaro Matsuzaki ; Narikazu Boku ; Seiichiro Abe ; Yutaka Saito ; Hiroyuki Daiko ; Satoko Takizawa ; Yoshiaki Aoki ; Hiromi Sakamoto ; Shumpei Niida ; Fumitaka Takeshita ; Takahiro Fukuda ; Takahiro OchiyaSeries Type : Non-coding RNA profiling by arrayOrganism : Homo sapiensA serum miRNA combination could be a powerful classifier for the detection of esophageal squamous cell carcinoma.
15h
GSE103108 Subpopulations of activated hepatic stellate cells defined and inhibited by WT1 expression
Series Type : Expression profiling by array ; Expression profiling by high throughput sequencingOrganism : Mus musculusThis SuperSeries is composed of the SubSeries listed below.
15h
Contributor : Timothy J KendallSeries Type : Expression profiling by arrayOrganism : Mus musculusTransciptional heterogeneity of scar-orchestrating cells in liver fibrosis, defined by WT1 expression, after chronic tetrachloride injury in vivo (WT1-high and WT1-low) or activation in vitro (WT1-negative).
15h
Contributors : Calais S Prince ; Nicole Noren Hooten ; Nicolle A Mode ; Yongqing Zhang ; Ngozi Ejiogu ; Kevin G Becker ; Alan B Zonderman ; Michele K EvansSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensFrailty is an aging-associated syndrome resulting from diminished capacity to respond to stressors, disrupted homeostasis. It is a significant risk factor for disability and mortality. Although frailty is usually studied in old age, it is present in mid-life....
15h
Contributors : Masazumi Nishimoto ; Masataka Hirasaki ; Yosuke Mizuno ; Akihiko OkudaSeries Type : Expression profiling by arrayOrganism : Mus musculusYAP is known as a proto-oncogene with which the aberrant expression causes numerous types of cancer. To elucidate the molecular mechanisms underlying the acquisition of cancerous traits of normal cells by YAP overexpression, the wild-type YAP or its stabilized form was overexpressed in NIH3T3 cells. We found that the forced expression of either...
15h
Contributors : Timothy J Kendall ; Stuart AitkenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusActivated hepatic stellate cells orchestrate scar formation during fibrotic liver injury. Recent evidence has shown limited quiescent precursor derivation from the mesoderm during development. Here, we use lineage-tracing from development, through adult homeostasis, to adult fibrotic injury, to show that discreet subpopulations of activated hepatic stellate cells...
15h
Contributor : Shmuel YaccobySeries Type : Expression profiling by arrayOrganism : Homo sapiensTo understand how interactions of myeloma cells with osteoclasts and mesenchymal stem cells in the bone marrow affect the clinical course of myeloma, we used microarrays to study changes in gene expression in freshly isolated myeloma plasma cells following co-cultures with osteoclasts (8 experiments) or with mesenchymal stem cells (13 experiments). Interaction with osteoclasts induced changes in the expression...
15h
Contributors : Wei Wang ; Raffaele Fronza ; Manfred SchmidtSeries Type : OtherOrganism : Homo sapiensHIV-1 recurrently targets active genes that are positioned in the outer shell of the nucleus and integrates in the proximity of the nuclear pore compartment. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained unclear. Here we show that recurrently targeted genes are delineated with super-enhancer genomic elements...
16h
Contributors : José E Pérez-Ortín ; José García-Martínez ; Antonia M Romero-Cuadrado ; Sergi P TodolíSeries Type : Expression profiling by arrayOrganism : Saccharomyces cerevisiaeThe goal of the project was to study the effects on transctiption and mRNA stability after iron depletion. We analyzed the effect of iron depletion caused by 100 μM of the Fe2+-specific chelator bathophenanthroline disulfonic acid (BPS) on the transcription rates, mRNA stabilities and mRNA levels by doing Genomic Run-On...
18h
Purpose. To present a digital method that combines intraoral and face scanning for the computer-assisted design/computer-assisted manufacturing (CAD/CAM) fabrication of implant-supported bars for maxillary overdentures. Methods. Over a 2-year period, all patients presenting to a private dental clinic with a removable complete denture in the maxilla, seeking rehabilitation with implants, were considered for inclusion in this study. Inclusion criteria were fully edentulous maxilla, functional problems...
33m
Electronegative low-density lipoprotein (LDL(-)) has been found in the plasma of familial hypercholesterolemia and acute myocardial infarction and has been implicated in atherosclerosis and cardiovascular disease. However, less is known about the involvement of LDL(-) in atherosclerosis-related inflammation. This study aims at investigating the inducibility of LDL(-) by atherogenic diet in rabbits and at exploring the proinflammatory potential of the diet-induced LDL(-) in macrophages. Rabbits were...
1h
Priapism may present as a side effect in patients treated with medications for erectile dysfunction, in which it should be controlled in a timely manner to avoid complications. There is little information regarding the use of local measures for the treatment of this condition. This study was done with the objective to describe the management of priapism secondary to erectile dysfunction drugs in a cohort of men. Records of emergencies and adverse events were reviewed by two researchers to identify...
2h
Wireless sensor networks (WSNs) deployed in harsh and unfavorable environments become inoperable because of the failure of multiple sensor nodes. This results into the division of WSNs into small disjoint networks and causes stoppage of the transmission to the sink node. Furthermore, the internodal collaboration among sensor nodes also gets disturbed. Internodal connectivity is essential for the usefulness of WSNs. The arrangement of this connectivity could be setup at the time of network startup....
4h
This paper presents a procedure to optimize the rise-to-span ratio of single-layer reticulated cylindrical roofs to improve their aerodynamic performance, by coupling the optimization method with computational fluid dynamics (CFD) and finite element analysis (FEA). Four turbulence models (standard k-ε, RNG k-ε, SST k-ω, and RSM) were used to predict the mean wind loads on cylindrical roofs. The simulation results were compared with wind tunnel data, and the RSM turbulence model was employed. The...
12h
Although mesenchymal stromal cells (MSCs) have demonstrated beneficial effects on experimental acute respiratory distress syndrome (ARDS), preconditioning may be required to potentiate their therapeutic effects. Additionally, administration of cell-free products, such as extracellular vesicles (EVs) obtained from MSC-conditioned media, might be as effective as MSCs. In this study, we comparatively evaluated the effects of MSCs, preconditioned or not with serum collected from mice with pulmonary or...
13h
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