- Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope PresentationA detailed method is provided here describing the purification, refolding, and characterization of self-assembling protein nanoparticles (SAPNs) for use in vaccine development.
- Incidence and impact of sepsis on long-term outcomes after subarachnoid hemorrhage: a prospective observational studyAneurysmal subarachnoid hemorrhage (SAH) is an acute cerebrovascular disease associated with high mortality and long-term functional impairment among survivors. Systemic inflammatory responses after acute inju...
- Rectal wall dose-volume effect of pre- or post KUSHEN Ningjiaos relationship with 3D brachytherapy in cervical cancer patientsThe present prospective study evaluated the safety and efficacy of the rectum following KUSHEN Ningjiaos in cervical cancer. We compared rectal wall changes during brachytherapy with or without KUSHEN Ningjiaos i...
- Quantification of Protein Interaction Network Dynamics using Multiplexed Co-ImmunoprecipitationQuantitative Multiplex Immunoprecipitation (QMI) uses flow cytometry for sensitive detection of differences in the abundance of targeted protein-protein interactions between two samples. QMI can be performed using a small amount of biomaterial, does not require genetically engineered tags, and can be adapted for any previously defined protein interaction network.
- Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell LinesThe presence of cancer stem cells have been associated with relapse or poor outcomes after radiotherapy. This manuscript describes the methods to study the radiosensitivity of cancer stem cells in lung cancer cell lines.
- Stroke in malignancy: complexities of diagnosis and management: a case reportAlthough there is an established association between cancer and stroke, the role of malignancy as a causative agent or comorbidity is not always clear. Moreover, there are no established guidelines on the acut...
- DEAD-box ATPases are global regulators of phase-separated organellesNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1502-yRNA-dependent DEAD-box ATPases (DDXs) regulate the dynamics of phase-separated organelles, with ATP-bound DDXs promoting phase separation, and ATP hydrolysis inducing compartment disassembly and RNA release.
- BCAA catabolism in brown fat controls energy homeostasis through SLC25A44Nature, Published online: 21 August 2019; doi:10.1038/s41586-019-1503-xThe solute carrier transporter protein SLC25A44 regulates uptake of branched-chain amino acids in mitochondria of brown adipose tissue in which they are utilized for thermogenesis.
- Identification of an ATP-sensitive potassium channel in mitochondriaNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1498-3The pore-forming and ATP-binding subunits of a mitochondrial protein complex that mediates ATP-dependent potassium currents are identified and characterized, revealing the role of this channel in mitochondrial physiology and pathologies.
- Quantifying inactive lithium in lithium metal batteriesNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1481-zTitration gas chromatography is developed as an analytical method of distinguishing between lithium metal and lithium compounds within a cycled battery and assessing the amount of unreacted metallic lithium available.
- Reconstituting the transcriptome and DNA methylome landscapes of human implantationNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1500-0Transcriptomics and DNA methylomics are used to study the implantation process of human embryos at single-cell resolution.
- Electron pairing in the pseudogap state revealed by shot noise in copper oxide junctionsNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1486-7Shot-noise measurements in copper oxides reveal paired charge carriers existing in the pseudogap above the superconducting critical temperature, shedding light on the properties of high-temperature superconductivity in these materials.
- Strategies for combating online hateNature, Published online: 21 August 2019; doi:10.1038/d41586-019-02447-1An analysis of the dynamics of online hate groups on social-media platforms reveals why current methods to ban hate content are ineffective, and provides the basis for four potential strategies to combat online hate.
- Soils linked to climate changeNature, Published online: 21 August 2019; doi:10.1038/d41586-019-02450-6Carbon has been stored in the organic layers of boreal-forest soils for hundreds of years. An analysis reveals that this carbon might be released into the atmosphere as global warming increases the frequency of wildfires.
- Microbiota-derived lantibiotic restores resistance against vancomycin-resistant <i>Enterococcus</i>Nature, Published online: 21 August 2019; doi:10.1038/s41586-019-1501-zThe gut commensal Blautia producta secretes a lantibiotic that reduces colonization of the gut by the major pathogen vancomycin-resistant Enterococcus faecium, and transplantation of microbiota with high abundance of the lantibiotic gene enhances resistance to colonization in mice.
- Conserved cell types with divergent features in human versus mouse cortexNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1506-7RNA-sequencing analysis of cells in the human cortex enabled identification of diverse cell types, revealing well-conserved architecture and homologous cell types as well as extensive differences when compared with datasets covering the analogous region of the mouse brain.
- Force-induced conformational changes in PIEZO1Nature, Published online: 21 August 2019; doi:10.1038/s41586-019-1499-2Cryo-electron microscopy and high-speed atomic force microscopy reveal that PIEZO1 can reversibly deform its shape towards a planar structure, which may explain how the PIEZO1 channel is gated in response to mechanical stimulation.
- Author Correction: Epoxyeicosatrienoic acids enhance embryonic haematopoiesis and adult marrow engraftmentNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1489-4Author Correction: Epoxyeicosatrienoic acids enhance embryonic haematopoiesis and adult marrow engraftment
- Droplet motion electrically controlledNature, Published online: 21 August 2019; doi:10.1038/d41586-019-02451-5The movement of small droplets on a substrate is governed by surface-tension forces. A technique that can tune the surface tension of robust oxide substrates for droplet manipulation could open up many applications.
- Increasing wildfires threaten historic carbon sink of boreal forest soilsNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1474-ySoil radiocarbon dating reveals that combusted ‘legacy carbon’—soil carbon that escaped burning during previous fires—could shift the carbon balance of boreal ecosystems, resulting in a positive climate feedback.
- Challenges in evidencing the earliest traces of lifeNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1436-4Abiotic processes can mimic or alter the biogenic traces of early life but advances in micro- and nanoscale analyses provide evidence that—with geological contextualization—improves our ability to address this issue.
- Hidden resilience and adaptive dynamics of the global online hate ecologyNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1494-7The dynamics of interactions between hate-orientated networks on different online platforms is characterized, and a mathematical model predicts that policing on one online platform can make matters worse and generate ‘darker’ parts of the Internet.
- Daily briefing: July was the hottest month ever recorded on EarthNature, Published online: 21 August 2019; doi:10.1038/d41586-019-02536-1Just one of several worrying climate records broken last month, why you should say no to peer review on holiday, and we check in on China’s bold plan to lead the world in AI.
- A mechanism for touchNature, Published online: 21 August 2019; doi:10.1038/d41586-019-02454-2Piezo proteins mediate the sense of touch. A near-complete structure of one such protein has been obtained, and the mechanism for converting mechanical signals into electrical ones has been probed in another.
- Podcast: Tackling online hate speech, and identifying early fossilsNature, Published online: 21 August 2019; doi:10.1038/d41586-019-02513-8Listen to this week’s science news, with Benjamin Thompson and Shamini Bundell.
- A recipe book for cell types in the human brainNature, Published online: 21 August 2019; doi:10.1038/d41586-019-02343-8Whether cell types in the brain have been conserved during evolution is not clear. A comparison of the molecular recipes that define brain cell types in humans and mice reveals similarities and differences between species.
- Ionic-surfactant-mediated electro-dewetting for digital microfluidicsNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1491-xA method of droplet manipulation is described that uses electrical signals to induce the liquid to dewet, rather than wet, a hydrophilic conductive surface.
- Mechanosensation of cyclical force by PIEZO1 is essential for innate immunityNature, Published online: 21 August 2019; doi:10.1038/s41586-019-1485-8PIEZO1 signalling mediates activation of a proinflammatory response to cyclical pressure fluctuations in immune cells.
- Pressure regulates immune-cell functionNature, Published online: 21 August 2019; doi:10.1038/d41586-019-02339-4Immune cells called monocytes enter the lung during infection. Whether they help to launch a defence response is affected by the pressure encountered there, which is sensed by an ion channel called PIEZO1.
- Structure and mechanogating of the mammalian tactile channel PIEZO2Nature, Published online: 21 August 2019; doi:10.1038/s41586-019-1505-8The cryo-electron microscopy structure of mouse PIEZO2 is determined and compared to that of PIEZO1, providing insights into the potential gating mechanisms of these mechanosensitive ion channels.
- A broad range of symptoms in allgrove syndrome: single center experience in Southeast AnatoliaAbstract Background Allgrove syndrome (OMIM 231550) is a rare autosomal recessive disease characterized by non-CAH primary adrenal insufficiency (non-CAH PAI), alacrima, and achalasia. It is caused by mutations in the AAAS gene. The syndrome is also associated with variable progressive neurological impairment and dermatological abnormalities.
- FTIR bio-spectroscopy scattering correction using natural biological characteristics of different cell linesAnalyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN00811J, PaperSara Hariri, Sahar Barzegari B, Kamyar Keshavarz F, Nastaran Nikounezhad, Behnoosh Safaei, Golrokh Farnam, Farshad Hosseini ShiraziFourier transform infrared (FTIR) spectroscopy is a well-known method of analysis, with various applications, including promising potential for analyzing biological samples. In the bio-spectroscopy of cells, Mie scattering may increase,...The content of
- Η υπέρταση σε νεαρή ηλικία θέτει σε κίνδυνο την υγεία του εγκεφάλουΗ υπέρταση στην κρίσιμη ηλικιακή περίοδο των 30 και 40 ετών μπορεί να βλάψει τον εγκέφαλο, σύμφωνα με νέα έρευνα την οποία αναδημοσιεύει το ειδησεογραφικό πρακτορείο ΒBC. Τα αποτελέσματα της παρούσας μελέτης που δημοσιεύτηκαν στο Lancet Neurology έδειξαν πως η υψηλή αρτηριακή πίεση στη μέση ηλικία σχετίζεται με καταστροφή των αιμοφόρων αγγείων και συρρίκνωση του εγκεφάλου. Συγκεκριμένα, βρέθηκε ότι η υπέρταση στις ηλικίες 36 με 43 συνδέεται με
- Αυτοί είναι πιο πιθανό να προδώσουν την εμπιστοσύνη σαςΕνώ το να ζουν οι άνθρωποι σε κοινωνίες και ομάδες έχει αποδειχθεί επωφελές, η πιθανότητα προδοσίας από κάποιο μέλος της «ομάδας» είναι πανταχού παρούσα. Αυτή η περίπλοκη κατάσταση του συμβιβασμού και της συμβίωσης με άλλους ανθρώπους δημιούργησε εξελικτικές πιέσεις για τη δημιουργία ενός συστήματος σκέψης, ικανού να προβλέπει τις συμπεριφορές και τις προθέσεις των άλλων. Κάπως έτσι οι άνθρωποι βασίζονται στην πεποίθηση ότι μπορούν να «διαβάσουν
- Pediatric thyroid cancer- A surgical challengePublication date: Available online 20 August 2019Source: European Journal of Surgical OncologyAuthor(s): Ashok R. Shaha, R. Michael Tuttle
- Appendiceal mucinous neoplasm mimics ovarian tumors: Challenges for preoperative and intraoperative diagnosis and clinical implicationPublication date: Available online 20 August 2019Source: European Journal of Surgical OncologyAuthor(s): Wei Zhang, Cong Tan, Midie Xu, Xiaohua WuAbstractObjectiveThe aim of this study was to investigate the clinicopathological characteristics of appendiceal mucinous neoplasm that had been preoperatively misdiagnosed as a mucinous ovarian tumor and to discuss the clinical impacts of misdiagnosis.MethodsSeventy-eight patients with a final patholog
- A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source ToolsHere, we describe a step-by-step strategy for isolating small RNAs, enriching for microRNAs, and preparing samples for high-throughput sequencing. We then describe how to process sequence reads and align them to microRNAs, using open source tools.
- Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial ScalesMicrobial biofilms form complex architectures at interphases and develop into highly scale-dependent spatial patterns. Here, we introduce an experimental system (hard- and software) for the automated acquisition of 3D optical coherence tomography (OCT) datasets. This toolset allows the non-invasive and multi-scale characterization of biofilm morphogenesis in space and time.
- Entstehung der ArterioskleroseZusammenfassung Unter dem Begriff „Arteriosklerose“ wird eine Gruppe von arteriellen Gefäßerkrankungen zusammengefasst, die aufgrund unterschiedlicher biologischer Prozesse zur Versteifung und zum Verlust der Elastizität der Gefäßwand führen. Die derzeit geläufigste Klassifizierung unterscheidet nach histopathologischen Kriterien 4 Typen: die Arteriolosklerose, die Mediasklerose, die fibromuskuläre Intimahyperplasie und die Ath
- Isolation of Tissue Extracellular Vesicles from the LiverThis is a protocol to isolate tissue extracellular vesicles (EVs) from the liver. The protocol describes a two-step process involving collagenase perfusion followed by differential ultracentrifugation to isolate liver tissue EVs.
- Global adaptation confounds the search for local adaptation [NEW RESULTS]Spatially varying selection promotes variance in allele frequencies, increasing genetic differentiation between the demes of a metapopulation. For that reason, outliers in the genome wide distribution of summary statistics measuring genetic differentiation, such as FST, are often interpreted as evidence for alleles which contribute to local adaptation. However, in spatially structured populations, the spread of beneficial mutations with spatially
- Tuberculose multirésistante : une prise en charge qui pèse lourd aux hôpitaux universitaires de StrasbourgPublication date: Available online 20 August 2019Source: Revue des Maladies RespiratoiresAuthor(s): M. Paz, X. Argemi, F. Schramm, M. Mielcarek, L. Kassegne, Y. Hansmann, P. FraisseRésuméIntroductionLa tuberculose multirésistante (TB-MR) constitue un véritable défi médical et social mais il existe de grandes disparités épidémiologiques régionales. L’objectif de notre étude est d’évaluer les grands traits épidémiologiques, sociodémographiques, cli
- [ASAP] Total Synthesis of the Diterpenoid Alkaloid Arcutinidine Using a Strategy Inspired by Chemical Network AnalysisJournal of the American Chemical SocietyDOI: 10.1021/jacs.9b05815
- [ASAP] Enantioselective, Lewis Base-Catalyzed, Intermolecular Sulfenoamination of AlkenesJournal of the American Chemical SocietyDOI: 10.1021/jacs.9b07019
- [ASAP] Asymmetric Total Synthesis of Arcutinidine, Arcutinine, and ArcutineJournal of the American Chemical SocietyDOI: 10.1021/jacs.9b05818
- [ASAP] Spotlights on Recent <italic toggle="yes">JACS</italic> PublicationsJournal of the American Chemical SocietyDOI: 10.1021/jacs.9b09008
- [ASAP] Total Synthesis and Structural Validation of Phosdiecin A via Asymmetric Alcohol-Mediated Carbonyl Reductive CouplingJournal of the American Chemical SocietyDOI: 10.1021/jacs.9b07512
- [ASAP] Stoichiometric Formation of Open-Shell [PtAu<sub>24</sub>(SC<sub>2</sub>H<sub>4</sub>Ph)<sub>18</sub>]<sup>-</sup> via Spontaneous Electron Proportionation between [PtAu<sub>24</sub>(SC<sub>2</sub>H<sub>4</sub>Ph)<sub>18</sub>]<sup>2–</sup> and [PtAu<sub>24</sub>(SC<sub>2</sub>H<sub>4</sub>Ph)<sub>18</sub>]<sup>0</sup>Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b06254
- [ASAP] Nonstationary Two-Dimensional Nuclear Magnetic Resonance: A Method for Studying Reaction Mechanisms in SituAnalytical ChemistryDOI: 10.1021/acs.analchem.9b02414
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