Πέμπτη 2 Απριλίου 2020

Knocking out matrix metalloproteinase 12 causes the accumulation of M2 macrophages in intestinal tumor microenvironment of mice

Knocking out matrix metalloproteinase 12 causes the accumulation of M2 macrophages in intestinal tumor microenvironment of mice:

262.jpg

Abstract

MMP12 is mainly secreted by macrophages, is involved in macrophage development, and decomposes the extracellular matrix. Herein, we investigated whether macrophages would change in the intestinal tumor microenvironment after MMP12 knockout. ApcMin/+;MMP12−/−mice were obtained by crossbreeding ApcMin/+ mice with MMP12 knockout mice (MMP12−/− mice). The data showed that the number and volume of intestinal tumors were significantly increased in ApcMin/+;MMP12−/− mice compared with ApcMin/+ mice. Additionally, the tumor biomarkers CA19-9, CEA, and β-catenin appeared relatively early in intestinal tumors in ApcMin/+;MMP12−/− mice. The results demonstrated that knocking out MMP12 accelerated the tumor growth and pathological process. On further investigation of its mechanism, the proportions of M2 macrophages in the spleen and among peritoneal macrophages were significantly up-regulated in ApcMin/+;MMP12−/− mice. Expression of M2 macrophage-related genes was up-regulated in tumor and peritoneal macrophages. The M2-related cytokine levels of IL-4 and IL-13 were increased in the serum of ApcMin/+;MMP12−/−mice. In vitro, bone marrow-derived M2 macrophages were obtained by treating bone marrow cells with IL-4 and IL-13, and these M2 macrophages secreted cytokines being changed. This finding reveals the crucial role of MMP12 in macrophage development and provides a new target for the control of macrophage polarization. Knocking out MMP12 causes intestinal M2 macrophage accumulation in tumor microenvironment, promoting the growth of intestinal tumors in ApcMin/+ mice.

1 σχόλιο:

  1. How I became a happy woman again
    With tears of joy and happiness I am giving out my testimony to all viewers on line, my problem with Stomach Cancer stage IB and HIV has caused me many pains and sadness especially in my family.
    I was so afraid of loosing my life, I suffered the embarrassment of visiting
    therapy hundreds of times, unfortunately they did not find a definitive solution to my problem, I cried all day and night, do I have to live my life this way? I searched all true the internet for care, I was scammed by internet fraudsters times without numbers… until a friend of mine who stays in the UK introduced me to a friend of hers who was cured of the same disease, and she introduced me to Dr Itua who cured her from Breast Cancer by this email/WhatsApp +2348149277967, drituaherbalcenter@gmail.com. I contacted him and he promised that all will be fine and I had faith.He sent me his herbal medicines through Courier service and i was instructed on how to drink it for three weeks to cure,I followed the instruction given to me and Today am a happy woman again. He cures all kind of diseases like,Brain cancer,Gestational trophoblastic disease,Head and neck cancer,Ovarian cancer,Hodgkin lymphoma,Herpes,,Liver cancer,Throat cancer,Syndrome Fibrodysplasia Ossificans ProgresS sclerosis,Alzheimer's disease,Chronic Diarrhea,Copd,Parkinson,Als,Adrenocortical carcinoma Infectious mononucleosis,Intestinal cancer,Thyroid Cancer,Uterine cancer,Fibroid,Angiopathy, Ataxia,Arthritis,Amyotrophic Lateral Sclerosis,Brain Tumor,Fibromyalgia,Fluoroquinolone ToxicityBladder cancer,Hiv,Esophageal cancer,Gallbladder cancer,Kidney cancer,Hpv,Lung cancer,Melanoma,Mesothelioma,Multiple myeloma,Neuroendocrine tumors
    Non-Hodgkin lymphoma,Oral cancer,Sinus cancer,Hepatitis A,B/C,Skin cancer,Soft tissue sarcoma,Appendix,Spinal cancer,Stomach cancer,Vaginal cancer,Vulvar cancer,Testicular cancer,Tach Diseases,Leukemia.

    ΑπάντησηΔιαγραφή