Tue Apr 21, 2020 16:28
The first total synthesis of the hypermodified RNA nucleoside galactosyl‐queuosine (galQ) was achieved. Moreover, the distribution of this nucleoside as well as mannosyl‐queuosine and queuosine was determined in tissues of newborn and adult mice, and in human cytosolic tRNAs. Abstract Queuosine (Q) is a hypermodified RNA nucleoside that is found in tRNAHis, tRNAAsn, tRNATyr, and tRNAAsp. It is located at the wobble position of the tRNA anticodon loop, where it can interact with U as well as...
Tue Apr 21, 2020 16:27
In rerum natura: Wild‐type cytochrome P450BM3 catalyzes the oxidation of hydrosilanes to silanols both in vivo and in vitro. Directed evolution was used to generate an efficient and selective biocatalyst that delivers a broad range of aryl‐ and alkyl‐substituted silanols. Computational studies revealed a sequence of H atom abstraction and OH rebound as the mechanism, in analogy to the native C−H hydroxylation activity. Abstract Compared to the biological world's rich chemistry for functionalizing...
Tue Apr 21, 2020 16:21
A vinyl cyclopropane rearrangement embedded in an iridium‐catalyzed hydrogen borrowing reaction, enabled the formation of substituted stereo‐defined cyclopentanes from Ph* methyl ketone and cyclopropyl alcohols. Mechanistic studies provide evidence for the ring‐expansion reaction being the result of a cascade based on oxidation of the cyclopropyl alcohols, followed by aldol condensation with the pentamethyl phenyl‐substituted ketone to form an enone bearing the vinyl cyclopropane. Subsequent single...
Tue Apr 21, 2020 13:26
Pathogenic bacteria belonging to the Burkholderia pseudomallei group cause severe infectious diseases such as glanders and melioidosis. Malleicyprols were identified as important bacterial virulence factors, yet the biosynthetic origin of their cyclopropanol warhead that is crucial for bioactivity has remained enigmatic. By a combination of mutational analysis and metabolomics we found that sulfonium acids, dimethylsulfoniumpropionate (DMSP) and gonyol, known as osmolytes and as crucial components...
Tue Apr 21, 2020 13:23
The synthesis of a drug delivery platform based on graphene was achieved through a step‐by‐step strategy of selective amine deprotection and functionalization. The multifunctional graphene platform, functionalized with indocyanine green, folic acid and doxorubicin showed an enhanced anticancer activity. The remarkable targeting capacity for cancer cells in combination with the synergistic effect of drug release and photothermal properties prove the great advantage of a combined chemo‐ and phototherapy...
Tue Apr 21, 2020 13:21
Steps towards new peptidomimetics: A bottom‐up approach was used to design a ladder‐rung mimic chemotype overlaying across β‐hairpins to perturb protein–protein interactions. The chemotype was evaluated on a databank of over 250 000 PDB entries followed by biological assays to probe the inhibition of uPA⋅uPAR protein interactions. Abstract Secondary structures tend to be recognizable because they have repeating structural motifs, but mimicry of these does not have to follow such well‐defined...
Mon Apr 20, 2020 22:00
In DNA points accumulation in nanoscale topography (DNA‐PAINT), capable of single‐molecule localization microscopy with sub‐10‐nm resolution, the high background stemming from the unbound fluorescent probes in solution limits the imaging speed and throughput. Here, we reductively cage the fluorescent DNA probes conjugated with a cyanine dye to hydrocyanine, acting as a photoactivatable dark state. The additional dark state from caging lowered the fluorescent background while enabling optically selective...
Mon Apr 20, 2020 19:14
Better late than never: The key linkage between a proline and an (E)‐dehydrobutyrine in vioprolide D was successfully tackled by a late‐stage double‐bond isomerization. The first member of this class of biologically potent depsipeptides has thus been efficiently synthesized (16 linear steps, 2.0 % overall yield). Abstract The first total synthesis of vioprolide D was accomplished in an overall yield of 2.0 % starting from methyl (2S)‐3‐benzyloxy‐2‐hydroxypropanoate (16 steps in the longest...
Mon Apr 20, 2020 17:08
Iron‐chelated semiconducting polycomplex nanoparticles (SPFeN) with the ability to induce ferroptosis and a near‐infrared photothermal effect were developed for photoacoustic imaging‐guided combination cancer therapy. Abstract This study reports the development of iron‐chelated semiconducting polycomplex nanoparticles (SPFeN) for photoacoustic (PA) imaging‐guided photothermal ferrotherapy of cancer. The hybrid polymeric nanoagent comprises a ferroptosis initiator (Fe3+) and an amphiphilic semiconducting...
Mon Apr 20, 2020 16:43
Mon Apr 20, 2020 16:42
A chemical methodology finally addresses the undefeated challenge of modular and precision engineering of a Lys residue in a native protein. The remarkable control over selectivity provides the first‐ever approach to target Lys beyond the protein‐defined reactivity hotspot. This technology unfolds new gateways and renders analytically pure protein bioconjugates and homogeneous antibody‐drug conjugates. Abstract The necessity for precision labeling of proteins emerged during the efforts to understand...
Mon Apr 20, 2020 16:42
Three‐layered hierarchical Cu2S@carbon@MoS2 nanoboxes are synthesized through a multistep template‐engaged strategy. With the unique shell architecture and complex composition, these Cu2S@carbon@MoS2 nanoboxes exhibit enhanced electrochemical properties as an anode material for sodium‐ion batteries. Abstract Hybrid materials, integrating the merits of individual components, are ideal structures for efficient sodium storage. However, the construction of hybrid structures with decent physical/electrochemical...
Mon Apr 20, 2020 13:10
The structures and reactivities of organic phosphinidene chalcogenides to a large degree have been mainly inferred from trapping or complexation experiments. Phosphinidene chalcogenides derivatives appear to be an elusive family of molecules that have been suggested as reactive intermediates in multiple organophosphorus reactions. The quest to isolate “free” phosphinidene chalcogenides remains a challenge in the field. Here, we present the synthesis, IR and UV/Vis spectroscopic identification...
Mon Apr 20, 2020 11:21
Glypiation is a common posttranslational modification of eukaryotic proteins involving the attachment of a glycosylphosphatidylinositol (GPI) glycolipid. GPIs contains a conserved phosphoglycan modified in cell‐ and tissue‐specific manner. GPI complexity suggests roles in biological processes and effects on the attached proteins, but the difficulties to get homogeneous material hinder the studies. Here, we disclose a one‐pot intein‐mediated ligation (OPL) to obtain GPI‐anchored proteins. The strategy...
Sun Apr 19, 2020 22:00
Abstract Purpose Os odontoideum is a rare anatomical and morphological variation of the odontoid process and associated with a range of symptoms such as spinal cord and vertebral artery injuries. This study aimed to evaluate the frequency of os odontoideum in Turkish cases by sagittal/coronal cervical magnetic resonance imaging (MRI) and computed tomography (CT) and analyze the relationship with age, gender and related symptoms....
Thu Apr 23, 2020 11:54
Abstract Purpose Pre-operative planning is widely used in orthopaedic surgery. In case of trauma with fracture or previous injury with malunion, the contralateral extremity is used as a surrogate for planning with an assumption of symmetry between sides. The aim of this study was to investigate femoral symmetricity in human adults. Methods ...
Thu Apr 23, 2020 11:54
Abstract Purpose Attention to the inclination of lamellas attached to the skull base, including the basal lamella of the middle turbinate, facilitates the intraoperative identification of each lamella without requiring the use of a navigation system. We classified the inclination between the lamella and the skull base in preoperative computed tomography (CT) images and examined the relationship between the lamellas attached to the skull...
Wed Apr 22, 2020 03:00
Journal of Investigative Medicine High Impact Case Reports, Volume 8, Issue , January-December 2020. Pembrolizumab is a humanized monoclonal antibody that targets the programmed cell death 1 protein (PD-1) receptor and blocks the inhibitory checkpoint interaction between PD-1 and its ligands. This interaction leads to the upregulation of effector T-cells and downregulating regulatory T-cell production. Although this mechanism is essential for the management of cancer, it may lead to decreased self-tolerance...
Thu Apr 23, 2020 09:07
Oncogenesis, Published online: 22 April 2020; doi:10.1038/s41389-020-0225-0Profiling of subcellular EGFR interactome reveals hnRNP A3 modulates nuclear EGFR localization
Wed Apr 22, 2020 03:00
Wed Apr 22, 2020 03:00
Abstract Pancreatic ductal adenocarcinoma can be a difficult imaging diagnosis early in its course given its subtle imaging findings such as focal pancreatic duct dilatation, abrupt duct cut-off, and encasement of vasculature. A variety of pancreatitidies have imaging findings that mimic pancreatic ductal adenocarcinoma and lead to mass formation making diagnosis even more difficult on imaging alone. These conditions include acute focal pancreatitis, chronic pancreatitis, autoimmune...
Thu Apr 23, 2020 06:50
Abstract Acute pancreatitis is an increasingly common condition and can result in significant morbidity and mortality. Contrast enhanced computed tomography (CECT) is the primary initial imaging modality in the characterization of acute pancreatitis. In this article, we provide sample CECT technical acquisition parameters for pancreatic imaging. We also review the classification systems for acute pancreatitis and give examples of common and uncommon complications of acute pancreatitis.
Thu Apr 23, 2020 06:50
Abstract Developmental anomalies of the pancreas include more common entities such as pancreas divisum and annular pancreas, and unusual entities such as heterotopic pancreas. While these anomalies can present a diagnostic challenge to the radiologist, when the pancreatic tissue involved in these processes develops pancreatitis, the radiographic appearance can present a diagnostic dilemma. Awareness of these pancreatic anomalies and familiarity with their appearance when inflamed...
Thu Apr 23, 2020 06:50
Abstract Computed tomography (CT) is the most commonly performed imaging test for acute pancreatitis. Nevertheless, magnetic resonance (MRI) imaging is useful in many specific situations. These include evaluating patients with acute pancreatitis who cannot receive iodinated CT contrast, elucidating the underlying cause of acute pancreatitis, assessing ductal disconnection and for guiding intervention of necrotic collections. Non-contrast MRI is superior to non-contrast CT and...
Thu Apr 23, 2020 06:50
Abstract Multidisciplinary collaboration (MDC) has been widely adopted in healthcare to optimize patient care. MDC brings several specialized healthcare providers to the table using several methods, including multidisciplinary meetings (MDMs), multidisciplinary clinics, teleconferences, and online multidisciplinary expert panels, to reach the goal of achieving the best diagnosis and treatment plan for complex diseases. Diagnosis and management of acute/chronic pancreatitis is...
Thu Apr 23, 2020 06:50
Abstract MRI and MRCP play an important role in the diagnosis of chronic pancreatitis (CP) by imaging pancreatic parenchyma and ducts. MRI/MRCP is more widely used than computed tomography (CT) for mild to moderate CP due to its increased sensitivity for pancreatic ductal and gland changes; however, it does not detect the calcifications seen in advanced CP. Quantitative MR imaging offers potential advantages over conventional qualitative imaging, including simplicity of analysis,...
Thu Apr 23, 2020 06:50
Abstract Purpose This study aims to evaluate the capacity of the clinical and radiological findings and the extrapancreatic necrotic tissue volume in early (2–6 days) computerized tomography (CT) images of patients diagnosed with AP to predict reliably the temporary or permanent organ failure and other local or systemic complications due to pancreatitis. Materials...
Thu Apr 23, 2020 06:50
Abstract Various pathologic neoplasms present in both childhood and adulthood. Multiple environmental and genetic factors with different targets at different ages have been implicated in prior literature. These tumors include Multi-Locular Cystic Renal Tumors (MCRT), Adrenocortical Carcinomas, Paragangliomas, Hepatic Hemangiomas, and Medullary Renal Cell Carcinomas. The pertinent imaging, pathological, and clinical features of these select entities are discussed to assist the General...
Wed Apr 22, 2020 03:00
Rejuvenation Research, Volume 23, Issue 2, Page 91-92, April 2020.
Fri Apr 17, 2020 10:00
Rejuvenation Research, Volume 23, Issue 2, Page 93-106, April 2020.
Fri Apr 17, 2020 10:00
Rejuvenation Research, Volume 23, Issue 2, Page 107-121, April 2020.
Fri Apr 17, 2020 10:00
Rejuvenation Research, Volume 23, Issue 2, Page 176-183, April 2020.
Fri Apr 17, 2020 10:00
Rejuvenation Research, Volume 23, Issue 2, Page 122-129, April 2020.
Fri Apr 17, 2020 10:00
Rejuvenation Research, Volume 23, Issue 2, Page 150-158, April 2020.
Fri Apr 17, 2020 10:00
Rejuvenation Research, Volume 23, Issue 2, Page 130-137, April 2020.
Fri Apr 17, 2020 10:00
Rejuvenation Research, Volume 23, Issue 2, Page 171-175, April 2020.
Fri Apr 17, 2020 10:00
Rejuvenation Research, Volume 23, Issue 2, Page 138-149, April 2020.
Fri Apr 17, 2020 10:00
Rejuvenation Research, Volume 23, Issue 2, Page 184-189, April 2020.
Fri Apr 17, 2020 10:00
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