- Tartrazine Removal from Aqueous Solution by HDTMA-Br-Modified Colombian BentoniteThe effect of pH, ionic strength (NaCl added), agitation speed, adsorbent mass, and contact time on the removal of tartrazine from an aqueous solution, using an organobentonite, has been studied. A complete factorial design 32 with two replicates was used to evaluate the influence of the dye concentration (30, 40, and 50 mg/L) and amount of adsorbent (25, 35, and 45 mg) on decolorization of the solution. Experimental data were evaluated with Desi
- Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding OligopeptidesIon mobility-mass spectrometry and molecular modeling techniques can characterize the selective metal chelating performance of designed metal-binding peptides and the copper-binding peptide methanobactin. Developing new classes of metal chelating peptides will help lead to therapeutics for diseases associated with metal ion misbalance.
- Rapid Isolation of Dorsal Root Ganglion MacrophagesHere we present a mechanical dissociation protocol to rapidly isolate macrophages from the dorsal root ganglion for phenotyping and functional analysis.
- Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon MeasurementsThis article presents a protocol and software tool for the quantification of uncertainties in the calibration and data analysis of a semi-continuous thermal-optical organic/elemental carbon analyzer.
- Application of 3D Printing in the Construction of Burr Hole Ring for Deep Brain Stimulation ImplantsHere, we present a protocol to demonstrate 3D printing in the construction of deep brain stimulation implants.
- Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of SialoglycanPresented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.
- Oxytocin is a principal hormone that exerts part of its effects by active fragmentsPublication date: Available online 6 September 2019Source: Medical HypothesesAuthor(s): Kerstin Uvnäs Moberg, Linda Handlin, Kathleen Kendall-Tackett, Maria PeterssonSummaryOxytocin is a nonapeptide consisting of a cyclic six amino-acid structure and a tail of three amino acids. It was originally known for its ability to induce milk ejection and to stimulate uterine contractions. More recently, oxytocin has been shown to stimulate social be
- Cortical bone distribution in the femoral neck of <em>Paranthropus robustus</em>Publication date: October 2019Source: Journal of Human Evolution, Volume 135Author(s): Marine Cazenave, José Braga, Anna Oettlé, Travis Rayne Pickering, Jason L. Heaton, Masato Nakatsukasa, J. Francis Thackeray, Frikkie de Beer, Jakobus Hoffman, Jean Dumoncel, Roberto MacchiarelliAbstractStudies of the australopith (Australopithecus and Paranthropus) proximal femur have increasingly integrated information from the local arrangement of the cortica
- Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV TargetsBreast milk transmits human immunodeficiency virus (HIV), though only ~15% of infants breastfed by HIV-infected mothers become infected. Breastfed infants ingest ~105−108 maternal leukocytes daily, though these cells are understudied. Here we describe the isolation of breast milk leukocytes and an analysis of their phagocytic capacity.
- Hierarchical and Programmable One-Pot Oligosaccharide SynthesisThis protocol demonstrates how to use the Auto-CHO software for hierarchical and programmable one-pot synthesis of oligosaccharides. It also describes the general procedure for RRV determination experiments and one-pot glycosylation of SSEA-4.
- A 4-D approach for amelioration of PeriodontitisPublication date: Available online 6 September 2019Source: Medical HypothesesAuthor(s): Pooja Jain, M. Aamir Mirza, Zeenat IqbalAbstractGlobal prevalence of the severe periodontitis is at the alarming stage and its association with the systemic complications is highly evident which cannot be neglected. An insight into the pathophysiology of the periodontitis reveals that the promising amelioration could only be envisaged with the 4-D/ multi-pron
- Antitumor effects of the electromagnetic resonant frequencies derived from the <sup>1</sup>H-NMR spectrum of Ph<sub>3</sub>Sn(Mercaptonicotinic)SnPh<sub>3</sub> complexPublication date: Available online 6 September 2019Source: Medical HypothesesAuthor(s): Ioannis I. Verginadis, Spyridon Ch. Karkabounas, Yannis V. Simos, Anastasia P. Velalopoulou, Dimitrios Peschos, Antonis Avdikos, Ioannis Zelovitis, Nikolaos Papadopoulos, Evangelia Dounousi, Vasilios Ragos, Angelos M. EvangelouAbstractThe aim of this article is to investigate the potential cytotoxic and antitumor effects of the resonant electromagnetic fields
- 3D-printed Nerve Conduit with Vascular Networks to Promote Peripheral Nerve RegenerationPublication date: Available online 6 September 2019Source: Medical HypothesesAuthor(s): Jie Tao, Yan He, Shuai Wang, Jian MaoAbstractPeripheral nerve regeneration remains a challenge in tissue engineering and regenerative medicine. However, the existing approaches have limited regenerative capability. 3D-printed nerve conduits with well-defined properties are potent tools to facilitate peripheral nerve regeneration after injuries. Meanwhile, the
- Medical knowledge integration and “systems medicine”: needs, ambitions, limitations and optionsPublication date: Available online 5 September 2019Source: Medical HypothesesAuthor(s): Felix Tretter, Henriette Löffler-StastkaAbstractMedicine today is an extremely heterogeneous field of knowledge, based on clinical observations and action knowledge and on data from the biological, behavioral and social sciences. We hypothesize at first that medicine suffers from a disciplinary hyper-diversity compared to the level of conceptual interdis
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