Quantitative real-time PCR (qPCR) has become a widely used approach to analyze the expression level of selected genes. However, owing to variations in cell types and drug treatments, a suitable reference gene should be selected according to special experimental design. In this study, we investigated the expression level of ten candidate reference genes in hepatoma carcinoma cell (HepG2) and human hepatocyte cell line (L02) treated with ethanol (EtOH), hydrogen peroxide (H2O2), acetaminophen (APAP),...
Tue Jul 14, 2020 18:35
The proteasome inhibitor bortezomib (BTZ) is a potent first-line anticancer drug for multiple myeloma; nonetheless, it induced peripheral neuropathy. It has been suggested that many cytokines may play a role in mediating neuropathic pain, but the underlying molecular mechanism is not fully understood. Recent studies have shown that neuropathic pain is closely related to the purinergic ligand-gated ion channel 7 receptor (P2X7R), one of the P2X receptors, which is richly expressed in glial cells....
Tue Jul 14, 2020 18:20
Endometriosis is one of the most frequent gynecological diseases in reproductive age women, but its etiology is not completely understood. Endometriosis is characterized by progesterone resistance, which has been explained in part by a decrease in the expression of the intracellular progesterone receptor in the ectopic endometrium. Progesterone action is also mediated by nongenomic mechanisms via membrane progesterone receptors (mPRs) that belong to the class II members of the progesterone and adipoQ...
Tue Jul 14, 2020 17:50
Background. Polymorphonuclear neutrophils (PMNs) play an essential role in the innate immune response, and their number increases after prolonged inflammatory diabetic wounds and prolonged wounds in older rats. The expression of CD80 and CD86 on PMNs confirms their participation in acquired immunity, wherein these molecules are involved in antigen presentation. Materials and Methods. We investigated CD80 and CD86 expression on PMNs by flow cytometry and analyzed the mRNA expression of neutrophil...
Tue Jul 14, 2020 17:20
Objective. An artificial liver support system (ALSS) is an effective therapy for patients with severe liver injury. A vasovagal reaction (VVR) is a common complication in various treatment settings but has not been reported previously in ALSS. Methods. This study retrospectively evaluated patients who suffered an ALSS-related VRR between January 2018 and June 2019. We collected data from VVR episodes including onset time, duration, changes in heart rate (HR) and blood pressure (BP), and drug treatment....
Tue Jul 14, 2020 09:35
Objective. High-fat-diet- (HFD-) induced hepatic cell apoptosis is common in mice with nonalcoholic fatty liver disease (NAFLD). We aim to investigate the effect of Ginsenoside Rb1 (GRb1) on hepatocyte apoptosis. Methods. C57BL/6J mice with HFD were used to induce a liver-injured model with cell apoptosis. In addition, GRb1 was used to treat HFD-induced apoptosis in a liver with or without inhibitor of peroxisome proliferator-activated receptor γ (PPAR-γ). Results. Compared with C57BL/6J mice with...
Tue Jul 14, 2020 09:35
Advanced glycation end products (AGEs) have been widely regarded as an important inducing factor in the pathogenesis of diabetic arteriosclerosis, and the proliferation and migration of vascular smooth muscle cells (VSMCs) are also involved in this process. However, it is not clear whether AGEs promote atherosclerosis by inducing the proliferation and migration of VSMCs. To figure out this question, this study investigated the effects of AGEs on the proliferation and migration of human aorta vascular...
Tue Jul 14, 2020 09:05
Hucul and Arabian horses differ in the physiological constitution and exposition to environmental conditions. Oxidative stress plays a pathogenic role in many diseases and enables further injuries. The objective of this study was to compare the levels of enzymatic and nonenzymatic oxidative stress markers in Hucul horses living in seminatural conditions and in commercially handled Arabian horses. We tested the serum samples for total superoxide dismutase (total SOD), Cu-Zn-superoxide dismutase (CuZnSOD),...
Tue Jul 14, 2020 08:50
Purpose. To assess the impact of sinogram-affirmed iterative reconstruction (SAFIRE) on risk category for coronary artery disease by combining coronary calcium score measurement and coronary CT angiography (CCTA). Materials and Methods. Eighty-nine patients (64.0% male) older than 18 years ( years) underwent coronary artery calcium scanning and prospectively ECG-triggered sequential CCTA examination. All raw data acquired in coronary artery calcium scanning were reconstructed by both filtered back...
Tue Jul 14, 2020 08:35
SUMMARY In this work, we introduce an algorithmic approach to generate microvascular networks starting from larger vessels that can be reconstructed without noticeable segmentation errors. Contrary to larger vessels, the reconstruction of fine‐scale components of microvascular networks shows significant segmentation errors, and an accurate mapping is time and cost intense. Thus there is a need for fast and reliable reconstruction algorithms yielding surrogate networks having similar stochastic properties...
Mon Jul 13, 2020 19:38
AP Photo / Bebeto Matthews As physicians and healthcare workers have toiled to treat COVID-19 patients over the past few months of the pandemic, it’s been a continuous struggle and challenge to adapt clinical care methodology as we learn more about the disease pathogenesis and its consequences over time. We have not yet had to deal with a pandemic and virus in this way, and tackling this unknown, but powerful microscopic enemy has been the concerted effort of many dedicated and ingenious minds....
00:14
Researchers at Northwestern University have developed a new biomaterial, selenomelanin, that can help protect people from radiation. The new substance, chemically synthesized and produced by bacteria, helps protect cells from radiation more effectively than other forms of melanin. One day, it may be used in sunscreens, medical treatments, or for long-term spaceflight as a radioprotective material. In medical imaging, air travel, and space travel, there is often unwanted exposure...
Tue Jul 14, 2020 23:59
Wearable bioelectronic devices that stick to the skin and measure things such as temperature, heart rhythms, and other vitals are typically complex devices that use modern materials to do their job. They tend to be difficult to manufacture, expensive, and fragile, and so are still not widely available. Incredibly, researchers at the University of Missouri have now come up with a way of using nothing but graphite pencils and office paper to create highly functional bioelectronic devices....
Tue Jul 14, 2020 18:47
A variety of practices have recently emerged which relate to both video games and gambling. These range from opening loot boxes, to esports betting, real-money video gaming, token wagering, and social casino spending. It is unknown either how harmful or how widespread many of these activities are. A sample of 1,081 adults from the UK aged 18+ was therefore recruited. This sample was purposively recruited via quota sampling to represent the UK population in terms of sex, age, and ethnicity. Engagement...
Tue Jul 14, 2020 03:00
Background Alternative splicing (AS) is an important mechanism for regulating gene expression and proteome diversity. Tumor-alternative splicing can reveal a large class of new splicing-associated potential new antigens that may affect the immune response and can be used for immunotherapy. Methods The RNA-seq transcriptome data and clinical information of stomach adenocarcinoma (STAD) cohort were downloaded from The...
Tue Jul 14, 2020 03:00
Background Herpesviral hematopoietic necrosis disease, caused by cyprinid herpesvirus-2 (CyHV-2), is responsible for massive mortalities in the aquaculture of goldfish, Carassius auratus. Permissive cell lines for the isolation and propagation of CyHV-2 have been established from various goldfish tissues by sacrificing the fish. Here, we report the development of a cell line, FtGF (Fantail Goldfish Fin), from caudal fin of goldfish using non-lethal sampling. We also describe a simple protocol for...
Tue Jul 14, 2020 03:00
Introduction The use of activity trackers has increased both among private consumers and in healthcare. It is therefore relevant to consider whether a consumer-graded activity tracker is comparable to or may substitute a research-graded activity tracker, which could further increase the use of activity trackers in healthcare and rehabilitation. Such use will require knowledge of their accuracy as the clinical implications may be significant. Studies have indicated that activity trackers are not...
Tue Jul 14, 2020 03:00
Paenibacillus triticisoli BJ-18, a N2-fixing bacterium, is able to promote plant growth, but the secondary metabolites that may play a role in promoting plant growth have never been characterized. In this study, untargeted metabolomics profiling of P. triticisoli BJ-18 indicated the existence of 101 known compounds, including N2-acetyl ornithine, which is the precursor of siderophores, plant growth regulators such as trehalose 6-phosphate, betaine and trigonelline, and other bioactive molecules...
Tue Jul 14, 2020 03:00
Beer is the most popular low-alcohol beverage consumed in large amounts in many countries each year. The brewing industry is an important global business with huge annual revenues. It is profitable and important for the economies of many countries around the world. The brewing process involves several steps, which lead to fermentation of sugars contained in malt and conversion thereof into alcohol and carbon dioxide by yeasts. Beer brewing generates substantial amounts of by-products. The three main...
Tue Jul 14, 2020 03:00
The secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor which plays important role in bacterial infection, inflammation, wound healing and epithelial proliferation. Dysregulation of SLPI has been reported in a variety of human cancers including glioblastoma, lung, breast, ovarian and colorectal carcinomas and is associated with tumor aggressiveness and metastatic potential. However, the pathogenic role of SLPI in colorectal cancer is still unclear. Here we showed that SLPI...
Tue Jul 14, 2020 03:00
Background Colorectal cancer is one of the most common cancers worldwide. Psychological morbidity has an important impact on quality of life and major clinical outcomes. Several data have shown that the immune system may be a key player on the relation between psychological features and cancer outcomes. Natural Killer (NK) cells have been shown to be influenced by psychological factors. The aim of this investigation was to assess the impact of anxiety, depression, and anger state,...
Tue Jul 14, 2020 03:00
Environmental DNA (eDNA) metabarcoding is a cost-effective novel approach to estimate biodiversity in an ecosystem. In this study, the MiFish pipeline was employed to test if the system methodology is sufficiently reliable to estimate fish biodiversity in Korean rivers. A total of 125 unique haplotypes and 73 species were identified at the species level from 16 water samples collected from a single survey in four Korean rivers (Hyeongsan, Taehwa, Seomjin, and Nakdong). Among the four rivers, the...
Tue Jul 14, 2020 03:00
Varied environmental conditions in coastal-inland zones tend to influence soil faunal communities. However, few studies have focused on the responses of soil fauna to environmental variations along the coastal-inland gradient. In order to better understand the aforementioned responses, a total of 80 soil macro-faunal samples were collected at the five different distances from the coastline of China’s Bohai Bay. The results revealed that the compositions, structural characteristics and diversity of...
Tue Jul 14, 2020 03:00
Background/Purpose Over the past two decades, ulcerative colitis (UC) has emerged in the Asia Pacific area, and its treatment goal has shifted from symptom relief to endoscopic remission. Endoscopy is the gold standard for the assessment of mucosal healing; however, it is an invasive method. Fecal calprotectin (FC) is a non-invasive stool-based inflammatory marker which has been used to monitor mucosal healing status, but it is expensive. By contrast, the immune fecal occult blood...
Tue Jul 14, 2020 03:00
Gastrointestinal myxosporean parasites from the genus Enteromyxum are known to cause severe disease, resulting in high mortalities in numerous species of cultured marine fishes globally. Originally described as Myxidium spp., they were transferred to a new genus, Enteromyxum, to emphasize their novel characteristics. Their retention in the family Myxidiidae at the time was warranted, but more comprehensive phylogenetic analyses have since demonstrated the need for a new family for these parasites....
Tue Jul 14, 2020 03:00
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s):
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Raphaël Moeckli, Jean-François Germond, Claude Bailat, François Bochud, Marie-Catherine Vozenin, Jean Bourhis
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Eric E. Klein
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Denái R. Milton, Steven H. Lin, Brian P. Hobbs
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Chirag Shah, Benjamin D. Smith, Trevor J. Royce
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Patricia van Marlen, Max Dahele, Michael Folkerts, Eric Abel, Ben J. Slotman, Wilko F.A.R. Verbakel
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Angel Velarde, Kirk Douglas Najera, Hiram Gay, William G. Powderly, Sasa Mutic, Jonathan Green, Jeff M. Michalski, Lauren Henke, Vicky de Falla, Eric Laugeman, Marcos Catu, Geoffrey D. Hugo, Bin Cai, Jacaranda van Rheenen
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Colleen A.F. Lawton
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Jonathan Paly, Eric M. Horwitz
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Steven E. Schild
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Shraddha M. Dalwadi, Angela Zhu
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Michael T. Milano, Michael D. Chan, Giuseppe Minniti, Jona A. Hattangadi-Gluth, Kristin J. Redmond, Scott G. Soltys
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s):
Tue Jul 14, 2020 15:15
Publication date: 1 August 2020Source: International Journal of Radiation Oncology*Biology*Physics, Volume 107, Issue 5Author(s): Austin J. Sim, Anna M. Laucis, Mudit Chowdhary, Fumiko Chino, Daniel W. Golden, Rahul D. Tendulkar
Tue Jul 14, 2020 15:15
Publication date: Available online 13 July 2020Source: International Journal of Radiation Oncology*Biology*PhysicsAuthor(s): Eugenia Mylona, Martin Ebert, Angel Kennedy, David Joseph, James Denham, Allison Steigler, Stephane Supiot, Oscar Acosta, Renaud de Crevoisier
Tue Jul 14, 2020 15:15
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