Medicine by Alexandros G. Sfakianakis,Αλέξανδρος Γ. Σφακιανάκης

Application of Atomic Force Microscopy to Detect Early Osteoarthritis

We present a method to investigate early osteoarthritic changes at the cellular level in articular cartilage by using atomic force microscopy (AFM).

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Sun May 24, 2020 21:45

This is a method to generate “scarless” recombinant vaccinia viruses using host-range selection and visual identification of recombinant viruses.

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

Sun May 24, 2020 21:45

In this protocol, we describe a stable, highly efficient differentiation strategy for the generation of postganglionic sympathetic neurons from human pluripotent stem cells. This model will make neurons available for the use of studies of multiple autonomic disorders.

The Effect of Anodization Parameters on the Aluminum Oxide Dielectric Layer of Thin-Film Transistors

Sun May 24, 2020 20:44

Anodization parameters for growth of the aluminum-oxide dielectric layer of zinc-oxide thin-film transistors (TFTs) are varied to determine the effects on the electrical parameter responses. Analysis of variance (ANOVA) is applied to a Plackett-Burman design of experiments (DOE) to determine the manufacturing conditions that result in optimized device performance.

Conducting Concurrent Electroencephalography and Functional Near-Infrared Spectroscopy Recordings with a Flanker Task

Sun May 24, 2020 20:44

The present protocol describes how to perform concurrent EEG and fNIRS recordings and how to inspect the relationship between the EEG and fNIRS data.

Treatment of Facial Deformities using 3D Planning and Printing of Patient-Specific Implants

Sun May 24, 2020 19:42

As technology develops and becomes more user-friendly, planning of operations and patient-specific surgical guides and fixation plates should be performed by the surgeon. We present a protocol for 3D planning of orthognathic skeletal movements and 3D planning and printing of patient-specific fixation plates and surgical guides.

Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response

Sat May 23, 2020 21:22

We detail a simple method to produce high-resolution time-lapse movies of Pseudomonas aeruginosa swarms that respond to bacteriophage (phage) and antibiotic stress using a flatbed document scanner. This procedure is a fast and simple method for monitoring swarming dynamics and may be adapted to study the motility and growth of other bacterial species.

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

Sat May 23, 2020 21:00

In this article, we describe, in detail, a protocol for the generation of neurosphere cultures from postnatal mouse neural stem cells derived from the main mouse neurogenic niches. Neurospheres are used to identify neural stem cells from brain tissue allowing the estimation of precursor cell numbers. Moreover, these 3D structures can be plated in differentiative conditions, giving rise to neurons, oligodendrocytes and astrocytes, allowing the study of cell fate.

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Sat May 23, 2020 20:23

The electrochemiluminescence immunoassay (ECLIA) is a novel approach for quantitative detection of endogenous and exogenously applied MeCP2 protein variants, which produces highly quantitative, accurate and reproducible measurements with low intra- and inter-assay error over a wide working range. Here, the protocol for the MeCP2-ECLIA in a 96-well format is described.

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

Fri May 22, 2020 20:00

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Cues Utilized by Dentists in the Early Detection of Oral Cancer and Oral Potentially Malignant Lesions: Findings from the National Dental PBRN

Publication date: Available online 23 May 2020Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral RadiologyAuthor(s): Alexander Ross Kerr, Michael E. Robinson, Cyril Meyerowitz, Douglas E. Morse, Maria L. Aguilar, Scott L. Tomar, Lisa Guerrero, Dianne Caprio, Linda M. Kaste, Sonia K. Makhija, Rahma Mungia, Linda Rasubala, Walter J. Psoter, National Dental PBRN Collaborative Group

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

Sun May 24, 2020 21:11

Granulomatous ulceration of the palate

Publication date: Available online 23 May 2020Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral RadiologyAuthor(s): Tamara Fernandes de Castro, Saygo Tomo, Alan Roger Santos-Silva, Daniel Galera Bernabé, Éder Ricardo Biasoli, Marcelo Macedo Crivelini, Ana Cláudia Okamoto, Glauco Issamu Miyahara

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

Sat May 23, 2020 19:56

Identification of single nucleotide pleomorphisms associated with periodontal disease in head and neck cancer irradiation patients by exome sequencing

Publication date: Available online 22 May 2020Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral RadiologyAuthor(s): Valentina Talevi, Jia Wen, Rajesh V. Lalla, Michael T. Brennan, Farah B. Mougeot, Jean-Luc C. Mougeot

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

Sat May 23, 2020 19:56

Referrals by general dental practitioners and medical practitioners to oral medicine specialists in New Zealand: a study to develop protocol guidelines

Publication date: Available online 22 May 2020Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral RadiologyAuthor(s): Guangzhao Guan, Jessica Lau, Vanessa Yew, Jiayun U, Wenwen Qu, Jacky Lam, Ajith Polonowita, Li Mei

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

Fri May 22, 2020 17:01

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Publication date: Available online 24 May 2020Source: Journal of Oral and Maxillofacial SurgeryAuthor(s): Kareem Mahmoud, Nadia Galal, Sherif Ali, Amr Gibaly, Mohamed ELBehairy, Mohamed Mounir

Effect of Decompression on Large Mandibular Cyst Invading Mandibular Canal: Three-Dimensional Analysis of Change of Cyst Volume and Mandibular Canal Length

Sun May 24, 2020 21:02

Publication date: Available online 23 May 2020Source: Journal of Oral and Maxillofacial SurgeryAuthor(s): Jin-yong Cho, Jin-woo Kim, Sung-beom Kim, Jaeyoung Ryu

Estimating the Financial Impact of Site-Neutral Payments for Outpatient Consultations in Oral and Maxillofacial Surgery

Sun May 24, 2020 21:02

Publication date: Available online 23 May 2020Source: Journal of Oral and Maxillofacial SurgeryAuthor(s): Nisarg A. Patel

Resident Education in the Time of a Global Pandemic: Development of the Collaborative OMS Virtual Inter-Institutional Didactic (COVID) Program

Sun May 24, 2020 21:02

Publication date: Available online 23 May 2020Source: Journal of Oral and Maxillofacial SurgeryAuthor(s): Justine Moe, Carolyn Brookes, Donita Dyalram, Roderick Kim, James Melville, Faisal Quereshy, Steven Roser, Salam Salman, Thomas Schlieve, Martin Steed, Elda Fisher

Sun May 24, 2020 21:02

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Publication date: July 2020Source: European Journal of Cancer, Volume 134Author(s): Olga Nigro, Graziella Pinotti, Federica De Galitiis, Francesca Romana Di Pietro, Raffaele Giusti, Marco Filetti, Melissa Bersanelli, Alessandro Lazzarin, Paola Bordi, Annamaria Catino, Pamela Pizzutilo, Domenico Galetta, Paolo Marchetti, Andrea Botticelli, Simone Scagnoli, Marco Russano, Daniele Santini, Mariangela Torniai, Rossana Berardi, Biagio Ricciuti

The EORTC-DeCOG nomogram adequately predicts outcomes of patients with sentinel node–positive melanoma without the need for completion lymph node dissection

Sun May 24, 2020 20:48

Publication date: July 2020Source: European Journal of Cancer, Volume 134Author(s): Daniëlle Verver, A. Rekkas, Claus Garbe, David van Klaveren, Alexander C.J. van Akkooi, Piotr Rutkowski, Barry W.E.M. Powell, Caroline Robert, Alessandro Testori, Barbara L. van Leeuwen, Astrid A.M. van der Veldt, Ulrich Keilholz, Rudolf Stadler, Alexander M.M. Eggermont, Cornelis Verhoef, Ulrike Leiter, Dirk J. Grünhagen

Long term outcomes of stereotactic body radiation therapy for hepatocellular carcinoma without macrovascular invasion

Sun May 24, 2020 20:48

Publication date: July 2020Source: European Journal of Cancer, Volume 134Author(s): Ashwathy Susan Mathew, Eshetu G. Atenafu, Dawn Owen, Chris Maurino, Anthony Brade, James Brierley, Robert Dinniwell, John Kim, Charles Cho, Jolie Ringash, Rebecca Wong, Kyle Cuneo, Mary Feng, Theodore S. Lawrence, Laura A. Dawson

Preclinical profile and phase I clinical trial of a novel androgen receptor antagonist GT0918 in castration-resistant prostate cancer

Sun May 24, 2020 20:48

Publication date: July 2020Source: European Journal of Cancer, Volume 134Author(s): Tie Zhou, Weidong Xu, Wei Zhang, Ye Sun, Honghua Yan, Xu Gao, Fubo Wang, Qianxiang Zhou, Jianguo Hou, Shancheng Ren, Qing Yang, Bo Yang, Chuanliang Xu, Qingqing Zhou, Meiyu Wang, Chunyun Chen, Yinghao Sun

Induction chemotherapy followed by cisplatin or cetuximab concomitant to radiotherapy for laryngeal/hypopharyngeal cancer: Long-term results of the TREMPLIN randomised GORTEC trial

Sun May 24, 2020 20:48

Publication date: July 2020Source: European Journal of Cancer, Volume 133Author(s): Guillaume Janoray, Yoann Pointreau, Marc Alfonsi, Christian Sire, Lionel Geoffrois, Dominique de Raucourt, Etienne Bardet, Marie-Hélène Calais, Pascal Garaud, Gilles Calais

Impact of different adjuvant treatment approaches on survival in stage III endometrial cancer: A population-based study

Sun May 24, 2020 20:48

Publication date: July 2020Source: European Journal of Cancer, Volume 133Author(s): Willem Jan van Weelden, Casper Reijnen, Florine A. Eggink, Dorry Boll, Petronella B. Ottevanger, Hetty A. van den Berg, Maaike A. van der Aa, Johanna M.A. Pijnenborg

Comment et pourquoi adapter le masque Décathlon EasyBreath® pour les soignants prenant en charge des patients infectés par le SARS-CoV-2?

Sun May 24, 2020 20:48

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Otorhinolaryngology

Publication date: Available online 23 May 2020Source: European Annals of Otorhinolaryngology, Head and Neck DiseasesAuthor(s): B. Thierry, C. Célérier, F. Simon, C. Lacroix, R.-H. Khonsari

Otorhinolaryngology

Sun May 24, 2020 19:59

Swallowing difficulties caused by a voice prosthesis in a laryngectomee

Publication date: Available online 21 May 2020Source: European Annals of Otorhinolaryngology, Head and Neck DiseasesAuthor(s): I. Brook, L. Cord, M. Orestes, B. Davidson

Otorhinolaryngology

Fri May 22, 2020 13:21

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Genes, Chromosomes and Cancer

High‐risk cytogenetics in multiple myeloma: Further scrutiny of deletions within the IGH gene region enhances risk stratification

Abstract Multiple myeloma is a clonal malignancy of plasma cells in the bone marrow. Risk stratification is partly based on cytogenetic findings that include abnormalities of the IGH locus as determined by fluorescence in situ hybridization (FISH), such as rearrangements that result in either standard‐risk or high‐risk gene fusions. IGH deletions have been evaluated as a group in multiple myeloma patients with respect to cumulative outcomes but have provided limited guidance. Whether these deletions...

Genes, Chromosomes and Cancer

Sun May 24, 2020 14:10

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Polymerization shrinkage kinetics and degree of conversion of commercial and experimental resin modified glass ionomer luting cements (RMGICs)

Publication date: Available online 24 May 2020Source: Dental MaterialsAuthor(s): Amani Agha, Sandra Parker, Mangala Patel

Peel bond strength between 3D printing tray materials and elastomeric impression/adhesive systems: A laboratory study

Sun May 24, 2020 19:51

Publication date: Available online 23 May 2020Source: Dental MaterialsAuthor(s): Y. Xu, F. Huettig, C. Schille, E. Schweizer, J. Geis-Gerstorfer, S. Spintzyk

Clinical performance and SEM marginal quality of extended posterior resin composite restorations after 12 years

Sun May 24, 2020 19:51

Publication date: Available online 22 May 2020Source: Dental MaterialsAuthor(s): Roland Frankenberger, Christian Reinelt, Christian Glatthöfer, Norbert Krämer

Bioactive amorphous magnesium phosphate-polyetheretherketone composite filaments for 3D printing

Sat May 23, 2020 17:45

Publication date: Available online 22 May 2020Source: Dental MaterialsAuthor(s): Prabaha Sikder, Jessica A. Ferreira, Ehsan Akbari Fakhrabadi, Karla Z. Kantorski, Matthew W. Liberatore, Marco C. Bottino, Sarit B. Bhaduri

Sat May 23, 2020 17:45

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The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment

Publication date: August 2020Source: Biomedicine & Pharmacotherapy, Volume 128Author(s): Yi Liu, Mingyan Ju, Zihan Wang, Jiaci Li, Chenyi Shao, Ting Fu, Yaqing Jing, Yuxia Zhao, Zhe Lv, Guang Li

Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis

Sun May 24, 2020 19:49

Publication date: August 2020Source: Biomedicine & Pharmacotherapy, Volume 128Author(s): Zhang Yuan, Shi Yi-Yun, Yu Hai-Yan

Berberine represses human gastric cancer cell growth <em>in vitro</em> and <em>in vivo</em> by inducing cytostatic autophagy via inhibition of MAPK/mTOR/p70S6K and Akt signaling pathways

Sun May 24, 2020 19:49

Publication date: August 2020Source: Biomedicine & Pharmacotherapy, Volume 128Author(s): Qiang Zhang, Xiaobing Wang, Shijie Cao, Yujie Sun, Xinya He, Benke Jiang, Yaqin Yu, Jingshi Duan, Feng Qiu, Ning Kang