Related ArticlesIdentification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C). J Vis Exp. 2020 Apr 29;(158): Authors: Tian TV, Vidal E, Graf T, Stik G Abstract During mammalian development, cell fates are determined through the establishment of regulatory networks that define the specificity, timing, and spatial patterns of gene expression. Embryoid bodies (EBs) derived from pluripotent stem...
Tue May 19, 2020 15:53
Related ArticlesMeasuring Real-time Drug Response in Organotypic Tumor Tissue Slices. J Vis Exp. 2020 May 02;(159): Authors: Nishida-Aoki N, Bondesson AJ, Gujral TS Abstract Tumor tissues are composed of cancerous cells, infiltrated immune cells, endothelial cells, fibroblasts, and extracellular matrix. This complex milieu constitutes the tumor microenvironment (TME) and can modulate response to therapy in vivo or drug response ex vivo. Conventional...
Tue May 19, 2020 15:53
Related ArticlesIn Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding. J Vis Exp. 2020 May 01;(159): Authors: Fan C, Lim LKP, Wu Z, Sharma B, Gan SQ, Liang K, Upton Z, Leavesley D Abstract It has been shown that in vivo tissues are highly crowded by proteins, nucleic acids, ribonucleoproteins, polysaccharides, etc. The following protocol applies a macromolecular crowding (MMC) technique to mimic this physiological...
Tue May 19, 2020 15:53
Related ArticlesElimination of Serotonergic Neurons by Stereotaxic Injection of 5,7-Dihydroxytryptamine in the Dorsal Raphe Nuclei of Mice. J Vis Exp. 2020 May 01;(159): Authors: Cao L, Zhang Z, Lu X, Wang G, Meng D, Liu C, Yun J, Xu T, Zhao C, Lu J Abstract Stereotaxic injection has been widely used for direct delivery of compounds or viruses to targeted brain areas in rodents. Direct targeting of serotonergic neurons in the dorsal raphe nucleus...
Tue May 19, 2020 15:53
Related ArticlesIn situ Hybridization for Sipunculus nudus Coelomic Fluid. J Vis Exp. 2020 May 01;(159): Authors: Li W, Yuan M, Wu Y Abstract In situ hybridization (ISH) is a very informative technique to present cellular distribution patterns of specific genes (e.g., mRNA and ncRNA) in tissues. The sipunculid worm Sipunculus nudus is a crucial fishery resource as it has high nutritional and medicinal values. Currently, the research on the molecular...
Tue May 19, 2020 15:53
Related ArticlesPaper-Based Preconcentration and Isolation of Microvesicles and Exosomes. J Vis Exp. 2020 Apr 29;(158): Authors: Lee D, Wee KW, Kim H, Han SI, Kwak S, Yoon DS, Lee JH Abstract Microvesicles and exosomes are small membranous vesicles released to the extracellular environment and circulated throughout the body. Because they contain various parental cell-derived biomolecules such as DNA, mRNA, miRNA, proteins, and lipids, their enrichment...
Tue May 19, 2020 15:53
Related ArticlesEffect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation. J Vis Exp. 2020 May 02;(159): Authors: Rangarajan R, Ketelson HA, Do R, McCanna DJ, Suko A, Enstone D, Subbaraman LN, Dantam J, Jones LW Abstract Artificial lipid-containing tear formulations are developed to reduce tear evaporation by the restoration of a deficient tear lipid layer. Artificial tear formulations...
Tue May 19, 2020 15:53
Related ArticlesDetermination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth Assay. J Vis Exp. 2020 Apr 29;(158): Authors: Key M, Bergmann A, Micchelli C, Thornton LB, Millard S, Dou Z Abstract Toxoplasma gondii is a protozoan pathogen that widely affects the human population. The current antibiotics used for treating clinical toxoplasmosis are limited. In addition, they exhibit adverse...
Tue May 19, 2020 15:53
Related ArticlesFlow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model. J Vis Exp. 2020 Apr 24;(158): Authors: Haubruck P, Colbath AC, Liu Y, Stoner S, Shu C, Little CB Abstract Osteoarthritis (OA) is one of the most prevalent musculoskeletal diseases, affecting patients suffering from pain and physical limitations. Recent evidence indicates a potential inflammatory...
Tue May 19, 2020 15:53
Related ArticlesMeasuring RAN Peptide Toxicity in C. elegans. J Vis Exp. 2020 Apr 30;(158): Authors: Rudich P, Snoznik C, Puleo N, Lamitina T Abstract C. elegans is commonly used to model age-related neurodegenerative diseases caused by repeat expansion mutations, such as Amyotrophic Lateral Sclerosis (ALS) and Huntington's disease. Recently, repeat expansion-containing RNA was shown to be the substrate for a novel type of protein translation called...
Tue May 19, 2020 15:53
Related ArticlesCombined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine. J Vis Exp. 2020 Apr 23;(158): Authors: Wickham RJ, Lehr M, Mitchell L, Addy NA Abstract Phasic dopamine (DA) release from the ventral tegmental area (VTA) to the nucleus accumbens plays a pivotal role in reward processing and reinforcement learning. Understanding how the diverse neuronal...
Tue May 19, 2020 15:53
Quantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry.
Related ArticlesQuantifying Human Norovirus Virus-like Particles Binding to Commensal Bacteria Using Flow Cytometry. J Vis Exp. 2020 Apr 29;(158): Authors: Madrigal JL, Jones MK Abstract Commensal bacteria are well established to impact infection of eukaryotic viruses. Direct binding between the pathogen and the host microbiome is responsible for altering infection for many of these viruses. Thus, characterizing the nature of virus-bacteria binding...
Tue May 19, 2020 15:53
Related ArticlesA Rat Model of Pressure Overload Induced Moderate Remodeling and Systolic Dysfunction as Opposed to Overt Systolic Heart Failure. J Vis Exp. 2020 Apr 30;(158): Authors: Chaanine AH, Navar LG, Delafontaine P Abstract In response to an injury, such as myocardial infarction, prolonged hypertension or a cardiotoxic agent, the heart initially adapts through the activation of signal transduction pathways, to counteract, in the short-term,...
Tue May 19, 2020 15:53
Related ArticlesEngineering Intracellular Protein Sensors in Mammalian Cells. J Vis Exp. 2020 Apr 28;(158): Authors: Duhkinova M, Crina C, Weiss R, Siciliano V Abstract Proteins can function as biomarkers of pathological conditions, such as neurodegenerative diseases, infections or metabolic syndromes. Engineering cells to sense and respond to these biomarkers may help the understanding of molecular mechanisms underlying pathologies, as well as to...
Tue May 19, 2020 15:53
Related ArticlesMethod to Obtain Pattern of Breathing in Senescent Mice through Unrestrained Barometric Plethysmography. J Vis Exp. 2020 Apr 28;(158): Authors: Receno CN, Cunningham CM, Eassa BE, Purdy R, DeRuisseau LR Abstract Unrestrained barometric plethysmography (UBP) is a method for quantifying the pattern of breathing in mice, where breathing frequency, tidal volume, and minute ventilation are routinely reported. Moreover, information can...
Tue May 19, 2020 15:53
Related ArticlesAssessment of Static Graviceptive Perception in the Roll-Plane using the Subjective Visual Vertical Paradigm. J Vis Exp. 2020 Apr 28;(158): Authors: Jäger FI, Platho-Elwischger K, Wiest G Abstract Vestibular disorders are among the most common syndromes in medicine. In recent years, new vestibular diagnostic systems have been introduced that allow the examination of all semicircular canals in the clinical setting. Assessment methods...
Tue May 19, 2020 15:53
Related ArticlesInflammatory Pseudotumor in the Nasal Cavity and Sinuses: A Case Report and Associated Literature Review. Ear Nose Throat J. 2020 May 18;:145561320924489 Authors: Li J, Wang M, Li W, Tao Y, Shi X Abstract Inflammatory pseudotumor (IP) is a clinically aggressive but histologically benign condition of unknown cause. It rarely appears in the nasal cavity and sinuses. Here, we describe a 24-year-old female with the main symptom of right...
Tue May 19, 2020 15:52
Related ArticlesImpact of the COVID-19 Pandemic on Otolaryngology Residency: A Real-Life Experience. Ear Nose Throat J. 2020 May 18;:145561320926291 Authors: De Luca P, Colacurcio V, De Bonis E, Petrosino M, Bisogno A, Troisi D, Calvanese M, Marra P Abstract The coronavirus disease (COVID-19) pandemic as been rapidly spreading worldwide. In our country, the entire Italian Healthcare System has been forced to adapt to this unprecedented condition...
Tue May 19, 2020 15:52
Diversity in Academic Otolaryngology: An Update and Recommendations for Moving From Words to Action.
Related ArticlesDiversity in Academic Otolaryngology: An Update and Recommendations for Moving From Words to Action. Ear Nose Throat J. 2020 May 18;:145561320922633 Authors: Smith JB, Chiu AG, Sykes KJ, Eck LP, Hierl AN, Villwock JA Abstract OBJECTIVES: (1) To investigate the state of diversity and inclusion initiatives in otolaryngology-head and neck surgery (OHNS) as compared to general surgery and (2) assess their effects on female representation...
Tue May 19, 2020 15:52
Related ArticlesPerioperative Considerations in Endoscopic Skull Base Surgery. Curr Otorhinolaryngol Rep. 2020 Mar 19;:1-7 Authors: Hussaini AS, Clark CM, DeKlotz TR Abstract Purpose of the Review: Present an overview of perioperative considerations specific to endoscopic skull base surgery necessary to maximize successful outcomes. Recent Findings: The majority of perioperative considerations for endoscopic skull base surgery lack strong supporting...
Tue May 19, 2020 15:48
Related ArticlesA Cadaveric Study on the Course of the Cervical Segment of the Internal Carotid Artery and Its Variations. Cureus. 2020 Apr 13;12(4):e7663 Authors: Arumugam S, Subbiah NK Abstract Introduction The internal carotid artery is the chief source of blood supply to the brain. Variations of the internal carotid artery, such as curved, kinking, and coiling, might result in significant neurovascular problems due to alterations in the dynamics...
Tue May 19, 2020 15:48
Related ArticlesA rare case of invasive sinonasal carcinosarcoma. Int J Surg Case Rep. 2020 May 07;70:243-248 Authors: de Souza Cruz EL, de Moraes ATL, de Souza Neves Filho F, Carneiro Junior JT, Cruz E Silva BT, Montalli VAM, de Melo Alves Júnior S, de Jesus Viana Pinheiro J Abstract INTRODUCTION: Carcinosarcomas are rare malignant neoplasms formed by embryonic tissues (ectoderm and mesoderm) and present remarkably aggressive character with unfavourable...
Tue May 19, 2020 15:45
Related ArticlesA rare case of an irreducible Pipkin II fracture-dislocation of the femoral head in a young patient following low-energy trauma. Int J Surg Case Rep. 2020 May 07;70:234-237 Authors: Fernandez M, Williams T, Dubrana F, Di Francia R Abstract INTRODUCTION: Fractures-dislocations of the femoral head are rare, and irreducible dislocations are even rarer. The functional prognosis is poor. PRESENTATION OF CASE: We report the case of...
Tue May 19, 2020 15:45
Related ArticlesSimultaneous surgical treatment tactics of acute destructive cholecystitis combined with choledocholithiasis: A case report. Int J Surg Case Rep. 2020 May 12;70:230-233 Authors: Zhumatayev DT, Baimakhanov АN, Abdykadyrov MK, Nurmakov DA, Raimkhanov AD, Smagulov АM, Abdiyev NM Abstract INTRODUCTION: The widespread development of minimally invasive treatment methods have expanded the choices and options available to surgeons to manage patients...
Tue May 19, 2020 15:45
Related ArticlesUse of Purastat, a novel haemostatic matrix based on self-assembling peptides in the prevention of nasopharyngeal adhesion formation. Int J Surg Case Rep. 2020 May 08;70:227-229 Authors: Wong E, Ho J, Smith M, Sritharan N, Riffat F, Smith MC Abstract INTRODUCTION: Purastat is a novel haemostatic agent that has recently been used effectively in nasal procedures, specifically in functional endoscopic endonasal surgery in the prevention...
Tue May 19, 2020 15:45
Related ArticlesMasson's tumor involving the hand: A case report. Int J Surg Case Rep. 2020 May 08;70:223-226 Authors: Almarghoub MA, Shah Mardan QNM, Alotaibi AS, Ahmed NK, Alqahtani MS Abstract INTRODUCTION: Masson's tumor or IPEH represents a rare exuberant endothelial proliferation within a thrombus through an uncomprehended phenomenon. Being reported for the 1st time in Saudi Arabia, plastic surgeons should keep it in the list of differential diagnosis....
Tue May 19, 2020 15:45
Related ArticlesLetter to the editor RE: "Conservative management of 11 weeks old cervical ectopic pregnancy with transvaginal ultrasound-guided combined methotrexate injection: Case report and literature review". Int J Surg Case Rep. 2020 May 08;70:221-222 Authors: Naem A, Al-Kurdy B PMID: 32422582 [PubMed - as supplied by publisher]
Tue May 19, 2020 15:45
Related ArticlesSingle-port laparoscopic appendectomy using a needle-type grasping forceps for acute uncomplicated appendicitis in children: Case series. Int J Surg Case Rep. 2020 May 06;70:216-220 Authors: Chen Y, Yuan JQ, Guo SG, Yang ZJ Abstract INTRODUCTION: Single-port laparoscopy has been used in any areas of surgery, including appendectomy, to reduce the operative stress and enhance postoperative recovery procedure. This paper introduces our attempt...
Tue May 19, 2020 15:45
Related ArticlesThe first malignant primary hepatic glomus tumor: A case report. Int J Surg Case Rep. 2020 May 11;70:197-200 Authors: Aversa JG, Monroe C, Levi A, Papanicolau-Sengos A, Kleiner DE, Hernandez JM Abstract INTRODUCTION: Glomus tumors (GTs) are rare neoplasms that originate from the modified smooth muscle cells of glomus bodies and occasionally arise from visceral primary sites. All previously reported primary hepatic GTs were benign. Here...
Tue May 19, 2020 15:45
Related ArticlesFunctional outcomes in bilateral upper limb Amelia patient with scoliosis post vertical expandable prosthetic titanium rib (VEPTR) application: A case report. Int J Surg Case Rep. 2020 Mar 07;70:193-196 Authors: AlMarshad A, AlMazrua I, Al-Haidey R, AlZayed Z Abstract INTRODUCTION: Congenital upper limb Amelia is one of the extremely rare conditions in the world. Defined as complete absence of a limb which may present as isolated defect...
Tue May 19, 2020 15:45
Related ArticlesLow grade appendiceal mucinous neoplasm mimicking an ovarian cyst: A case report. Int J Surg Case Rep. 2020 May 07;70:145-148 Authors: Alghamdi AO, Aldossary MY, Alsawidan M, AlBahar S Abstract INTRODUCTION: Appendiceal mucocele (AM) is a very rare neoplasm with an incidence of 0.2-0.3% of all appendectomy specimens. AM usually defined as an intraluminal dilation of the appendix in response to mucin accumulation. The preoperative diagnosis...
Tue May 19, 2020 15:45
Related ArticlesTension band suture in isolated tibial tubercle avulsion: A case report and review literatures. Int J Surg Case Rep. 2020 May 08;71:1-5 Authors: Parinyakhup W, Boonriong T Abstract INTRODUCTION: Isolated tibial tubercle avulsion in adults are extremely rare, with only a few cases having been reported. The common treatment is screw fixation, followed by immobilization. We report a rare case of an isolated tibial tubercle avulsion in an...
Tue May 19, 2020 15:45
Related ArticlesAnnual Disease Experience by Type and Correlations with Unmet Healthcare Needs among ROK Military Personnel. Mil Med. 2020 May 18;: Authors: Kim HJ, Oh SSY, Choi DW, Won SY, Kim HJ, Ko SC, Jang SI, Park EC Abstract INTRODUCTION: The National Statistical Yearbook of Defense 2018 issued by the Republic of Korea (ROK) Ministry of National Defense reported that the number of patients using military hospitals steadily increased from 2008 to...
Tue May 19, 2020 15:41
Related ArticlesSpontaneous Ovarian Hyperstimulation Syndrome in a Partial Molar Pregnancy With Early Onset Severe Pre-eclampsia at 15 Weeks Gestation. Mil Med. 2020 May 18;: Authors: Davies MJ, Fletcher TL, Cox Bauer CM Abstract Ovarian hyperstimulation syndrome is a well-known entity in assisted reproductive technology. However, it is unusual for this entity to occur without any medications that stimulate follicle stimulating hormone. Herein, we describe...
Tue May 19, 2020 15:41
Publication date: July 2020Source: World Neurosurgery, Volume 139Author(s): Riyaq Abdi Farah, Ahmad Alduaij, Carlos Ugas, Ramon Navarro
Tue May 19, 2020 15:41
Publication date: July 2020Source: World Neurosurgery, Volume 139Author(s): Tomasz Klepinowski, Paweł Kawalec, Michał Larysz, Leszek Sagan
Tue May 19, 2020 15:41
Publication date: July 2020Source: World Neurosurgery, Volume 139Author(s): Monica C. Mureb, Yosef Dastazirgada, Carolina Benjamin, John G. Golfinos, Douglas Kondziolka
Tue May 19, 2020 15:41
Publication date: July 2020Source: World Neurosurgery, Volume 139Author(s): Nasser Almujaiwel, Ghadeer Alsager, Abdulaziz Oqalaa Almubarak, Abdulrazag Ajlan
Tue May 19, 2020 15:41
Publication date: July 2020Source: World Neurosurgery, Volume 139Author(s): Changik Lee, Yong Sam Shin, Jai Ho Choi
Tue May 19, 2020 15:41
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