Abstract D-p-hydroxyphenylglycine (D-HPG) functions as an intermediate and has important value in antibiotic industries. The high pollution and costs from chemical processes make biotechnological route for D-HPG highly desirable. Here, a whole-cell transformation process by D-hydantoinase(Hase) and D-carbamoylase(Case) was developed to produce D-HPG from DL-hydroxyphenylhydantoin(DL-HPH) in Escherichia coli. The artificially designed ribosome binding site with strong intensity...
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Abstract Wastewater treatment plants (WWTPs) rely mainly on the microbial assemblages to contribute significantly for the removal of organic pollutants and nutrients. However, limited information is available on the ecological driving forces underlying the turnover of prokaryotic communities across wastewater treatment processes (i.e., from influents (IFs) and effluents (EFs)) within WWTPs. Here, we used a combination of the 16S rRNA gene amplicon sequencing and a quantitative...
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Abstract Microbial xylanases have gathered great attention due to their biotechnological potential at industrial scale for many processes. A variety of lignocellulosic materials, such as sugarcane bagasse, rice straw, rice bran, wheat straw, wheat bran, corn cob, and ragi bran, are used for xylanase production which also solved the great issue of solid waste management. Both solid-state and submerged fermentation have been used for xylanase production controlled by various physical...
Tue Oct 22, 2019 03:00
Abstract Monoclonal antibodies (mABs) are of great biopharmaceutical importance for the diagnosis and treatment of diseases. However, their production in mammalian expression hosts usually requires extensive production times and is expensive. Escherichia coli has become a new platform for production of functional small antibody fragment variants. In this study, we have used a rhamnose-inducible expression system that allows precise control of protein expression levels. The system...
Tue Oct 22, 2019 03:00
Abstract Optimization of export mechanisms for valuable extracellular products is important for the development of efficient microbial production processes. Identification of the relevant export mechanism is the prerequisite step for product export optimization. In this work, we identified transporters involved in malate export in an engineered l-malate-producing Escherichia coli strain using cheminformatics-guided genetics tests. Among all short-chain di- or tricarboxylates with...
Tue Oct 22, 2019 03:00
International Journal of Molecular Sciences , IJMS, Vol. 20, Pages 5244: Transcription Factor... International Journal of Molecular Sciences , IJMS, Vol. 20, Pages 5244: Transcription Factor GarWRKY5 Is Involved in Salt Stress Response in Diploid Cotton Species (Gossypium aridum L.)IJMS, Vol. 20, Pages 5244: Transcription Factor GarWRKY5 Is Involved in Salt Stress Response in Diploid Cotton Species (Gossypium aridum L.) International Journal of Molecular Sciences doi: 10.3390/ijms20215244 Authors:...
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Abstract Background Temporal spatial parameters during running are measurable outside of clinical and laboratory environments using wearable technology. Data from wearable technology may be useful for injury prevention, however the association of temporal spatial parameters with overuse injury in runners remains unclear. Objective To identify the association...
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Abstract Although it is well understood that dehydration can have a major impact on exercise performance and thermoregulatory physiology, the potential for interactions between female sex hormone influences and the impact of dehydration on these variables is poorly understood. Female reproductive hormonal profiles over the course of the menstrual cycle have significant influences on thermoregulatory and volume regulatory physiology. Increased insight into the interactions among...
Tue Oct 22, 2019 03:00
Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN01753D, PaperKunhao Qin, Dongfang Zhang, Yafang Ding, Xiaodan Zheng, Yingying Xiang, Jianhao Hua, Qi Zhang, Xiuling Ji, Bo Li, Yunlin WeiCarbon dots (CDs) has broad prospective applications in various fields, and expanding the applications of fluorescent CDs, especially for CDs derived from bacteria, is a major research goal. In this...The content of this RSS Feed (c) The Royal Society of Chemistry
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Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN01541H, PaperJing Wang, Xu Xu, Xiaolin Qiu, Shuaishuai Zhang, Yinxian PengA yolk−shell structured Au@Ag@mSiO2 probe was fabricated by coating a layer of mesoporous silica on the surface of Au@Ag core-shell nanosphere, followed by partially removing the Ag shell. Mirror symmetrical...The content of this RSS Feed (c) The Royal Society of Chemistry
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Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN01680E, PaperJinying Liu, Xinhe Duan, Mengke Wang, Xingguang Suα-glucosidase and its inhibitors play a key role in diagnosis and treatment of diabetes. In the present work, we established a facile, sensitive and selective fluorescent method based on silicon...The content of this RSS Feed (c) The Royal Society of Chemistry
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Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN01561B, CommunicationYang Bu, Zisun Ahmed, Levent YobasThis paper reports the very first nanofluidic memristor based on the principle of ion concentration polarization (ICP). The ICP is induced through highly-ordered cylindrical nanochannels. These so-called nanocapillaries are formed...The content of this RSS Feed (c) The Royal Society of Chemistry
Tue Oct 22, 2019 02:00
Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN01339C, PaperAhmad Alassaf, Gulistan Tansik, Vera Mayo, Laura Wubker, Daniel Carbonero, Ashutosh AgarwalStandard culture of cardiac cells as unorganized monolayers on tissue culture plastic or glass does not recapitulate the architectural or the mechanical properties of native myocardium. We investigated physical and...The content of this RSS Feed (c) The Royal Society of Chemistry
Tue Oct 22, 2019 02:00
Analyst, 2019, Advance ArticleDOI: 10.1039/C9AN01362H, PaperAlula Yohannes, Shun YaoThe application of ionic liquids (ILs) for the separation of bioactive compounds from various sample matrices is a burgeoning area.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry
Tue Oct 22, 2019 02:00
Analyst, 2019, Accepted ManuscriptDOI: 10.1039/C9AN01478K, PaperGiovanni Pugliese, Phillip Trefz, Beate Brock, Jochen Schubert , Wolfram MiekischReactive exhaled volatile organic compounds (VOCs) such as nitrogen- and sulfur-containing substances may be related to disease, metabolic processes and bacterial activity. As these compounds may interact with any surface...The content of this RSS Feed (c) The Royal Society of Chemistry
Tue Oct 22, 2019 02:00
The eyespot patterns found on the wings of nymphalid butterflies are novel serial homologous traits that originated first in hindwings and subsequently in forewings, suggesting that eyespot development might be dependent on Hox genes. Hindwings differ from forewings in the expression of Ultrabithorax (Ubx), but the function of this Hox gene in eyespot development as well as that of another Hox gene Antennapedia (Antp), expressed specifically in eyespots centers on both wings, are still unclear. We...
Tue Oct 22, 2019 03:00
In Fisher's model of sexual selection, a female preference for a male trait spreads together with the trait because their genetic bases become correlated. This can be interpreted as a 'greenbeard' system: a preference gene, by inducing a female to mate with a trait-bearing male, favors itself because the male is disproportionately likely also to carry the preference gene. Here, we use this logic to argue that Fisherian sexual selection in diploids proceeds via two channels, corresponding to two reasons...
Tue Oct 22, 2019 03:00
Previous phylogeographic studies of the lion (Panthera leo) have offered increased insight into the distribution of genetic diversity, as well as a revised taxonomy which now distinguishes between a northern (Panthera leo leo) and a southern (Panthera leo melanochaita) subspecies. However, existing whole range phylogeographic studies on lions focused on mitochondrial DNA and/or a limited set of microsatellites. The geographic extent of genetic lineages and their phylogenetic relationships remain...
Tue Oct 22, 2019 03:00
One of the parental diploid genomes (subgenomes) in an allopolyploid often exhibits higher gene expression levels compared to the other subgenome(s) in the nucleus. However, the genetic basis and deterministic fate of subgenome expression dominance remains poorly understood. We examined the establishment of subgenome expression dominance in six isogenic resynthesized Brassica napus (rapeseed) allopolyploid lines over the first ten generations, and uncovered consistent expression dominance patterns...
Tue Oct 22, 2019 03:00
Natural selection can remove maladaptive genotypic variance from populations, leaving reduced phenotypic variation as a signal of its action. Hybrid populations offer a unique opportunity to study phenotypic variance before selection purifies it, as these populations can have increased genotypic and phenotypic variance than can reveal trade-offs and selection conflicts not visible, or visible to a lesser extent, in unadmixed populations. Here, we study the interactions between a fungal leaf rust...
Tue Oct 22, 2019 03:00
Sexually selected traits are hypothesized to be honest signals of individual quality due to the costs associated with their development or expression. Testosterone, a sex steroid known to influence the production of sexually selected traits, has been proposed to underlie the costs associated with sexually selected traits via its immunosuppressive effects (i.e., the Immunocompetence Handicap Hypothesis) or by influencing an individual's exposure/susceptibility to oxidative stress (i.e., the Oxidation...
Tue Oct 22, 2019 03:00
Malaria, a vector borne disease caused by Plasmodium spp., remains a major global cause of morbidity and mortality. Optimization of the disease control strategies requires a thorough understanding of the fundamental processes underlying parasite transmission. Although the number of transmissible stages of Plasmodium (gametocyte) in human blood is frequently used as an indicator of human-to-mosquito transmission potential, this relationship is not always clear. Important efforts have been made to...
Tue Oct 22, 2019 03:00
The Galapagos Islands are home to incredible endemic biodiversity that is of high conservation interest. Two such endemic species are the Galapagos tomatoes: Solanum cheesmaniae and Solanum galapagense. Both are known from historical location records, but like many endemic plant species on the Galapagos, their current conservation status is unclear. We revisited previously documented sites of endemic species on San Cristobal, Santa Cruz, and Isabela, and document the disappearance of >80% of these...
Tue Oct 22, 2019 03:00
Precise estimation of genetic substitution patterns is critical for accurate reconstruction of pathogen phylogenies. Few studies of viral evolution account for variations of mutation rate across a single gene. This is especially true when considering evolution of segmented viruses where individual segments are short, encoding for few proteins. However, the structural and functional partitions of these proteins could provide valuable information for more accurate inference of viral evolution, due...
Tue Oct 22, 2019 03:00
A central tenet of the evolutionary theory of communities is that competition impacts evolutionary processes such as local adaptation. Species in a community exert a selection pressure on other species and may drive them to extinction. We know, however, very little about the influence of unsuccessful or ghost species on the evolutionary dynamics within the community. Here, we studied the long-term influence of a ghost competitor on the performance of a more successful species using experimental evolution....
Tue Oct 22, 2019 03:00
Organisms vary in their dispersal abilities, and these differences can have important biological consequences, such as impacting the likelihood of hybridization events. However, the factors shaping the frequency of hybridization are still poorly understood, and therefore how dispersal ability affects the opportunities for hybridization is still unknown. Here, using the ecological replicate system of dove wing and body lice (Insecta: Phthiraptera), we show that species with higher dispersal abilities...
Tue Oct 22, 2019 03:00
The rapid evolution of influenza is an important contributing factor to its high worldwide incidence. The emergence and spread of genetic point mutations has been thoroughly studied both within populations and within individual hosts. In addition, influenza viruses are also known to generate genomic variation during their replication in the form of defective viral genomes (DVGs). These DVGs are formed by internal deletions in at least one gene segment that render them incapable of replication without...
Tue Oct 22, 2019 03:00
A holobiont is a composite organism consisting of a host together with its microbiome, such as a coral with its zooxanthellae. Hosts and their microbiomes are often intimately integrated in behavior, physiology, anatomy, and development. To explain this integration, some investigators contend that selection operates on holobionts as a unit and view the microbiome's genes as extending the host's nuclear genome to jointly comprise a hologenome. Holobiont selection then operates on holobionts with their...
Tue Oct 22, 2019 03:00
Stem Cells and Development, Ahead of Print.
Tue Oct 22, 2019 10:00
Stem Cells and Development, Ahead of Print.
Tue Oct 22, 2019 10:00
Related ArticlesEffect of four different finishing and polishing systems on resin composites: roughness surface and gloss retention evaluations. Minerva Stomatol. 2019 Oct 15;: Authors: Tosco V, Monterubbianesi R, Orilisi G, Procaccini M, Grandini S, Putignano A, Orsini G Abstract BACKGROUND: Finishing and polishing procedures play a crucial role to achieve the best aesthetic result of direct restorations. Many manufacturers to the clinicians offer different...
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Related ArticlesEvaluation of bond strength of dual resin cements to CAD/CAM-created lithium disilicate ceramic. Minerva Stomatol. 2019 Oct 15;: Authors: Sczepanski F, Brunnquell CR, Berger SB, Paloco EA, Lopes MB, Drumond AC, González AH, Guiraldo RD Abstract AIM: The objective was to examine the microshear bond strength values of different dual resin cements to computer-aided design (CAD)/computer-aided manufacturing (CAM)-created ceramics containing...
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Related ArticlesTemperature variations in pulp chamber: an in vitro comparison between ultrasonic and rotating instruments in tooth preparation. Minerva Stomatol. 2019 Oct 15;: Authors: Baldi D, Colombo J, Robiony M, Menini M, Bisagni E, Pera P Abstract BACKGROUND: The purpose of this study was to analyze pulpal temperature increase generated by prosthodontic margin repositioning and finishing with ultrasonic and rotating instruments. The temperature...
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Related ArticlesNovel hollow fiber sandwich composite post system: mechanical characteristics. Minerva Stomatol. 2019 Oct 15;: Authors: Bovolato L, Tonini R, Boschi G, Cavalli G, Salgarello SA Abstract BACKGROUND: There is currently no consensus on the optimal post system to provide effective long-term results. We used an engineering approach to investigate the mechanical properties of a sandwich hollow carbon fiber post in synergy with a new bonding...
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Facial plast Surg 2019; 35: 476-485DOI: 10.1055/s-0039-1696990The ability of a surgeon to create an aesthetically pleasing and functionally competent nasal dorsum weighs heavily on following the foundations of rhinoplasty, beginning with the examination and analysis. These same fundamental principles should be used and applied in secondary rhinoplasty. The most common chief complaints of those patients seeking primary rhinoplasty relates to the presence of a hump or dorsal convexity, and similarly...
Tue Oct 22, 2019 02:00
Facial plast Surg 2019; 35: 516-524DOI: 10.1055/s-0039-1696655Achieving a more youthful neckline is one of the most common reasons why patients seek facial plastic surgery. A variety of treatment options are available to contour the neck, ranging from injectable medications to minimally invasive energy devices to surgery. A spectrum of surgical procedures can be offered to contour the neck, including liposuction, submentoplasty, necklift, and facelift, all with or without implants. The ideal neck...
Tue Oct 22, 2019 02:00
Facial plast Surg 2019; 35: 423-429DOI: 10.1055/s-0039-1696654The facial profile is an essential part of facial analysis and significantly impacts surgical decision making. Knowledge of the underlying anatomy, which creates the facial profile, and the anatomic sources of the common abnormalities in the profile can help to ensure adequate surgical correction of the abnormality.[...]Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.Article in Thieme eJournals:Table of contents | Abstract | Full...
Tue Oct 22, 2019 02:00
Facial plast Surg 2019; 35: 492-498DOI: 10.1055/s-0039-1695726Nasal profile line has central importance in forming an aesthetically balanced facial profile. Thus, rhinoplasty is a critically necessary surgery in shaping ideal profile line. For successful rhinoplasty, meticulous preoperative evaluation and proper planning should be performed before surgery. The nasal dorsum is the broadest and most prominent part of the nose; any minor imperfection is readily appreciated by an observer. Thus, dorsal...
Tue Oct 22, 2019 02:00
Facial plast Surg 2019; 35: 430-445DOI: 10.1055/s-0039-1697962After studying this article, the reader should be able to understand the basic concepts of aesthetic reshaping of the forehead and the different methods to make those upper facial contour changes. The operative techniques for bony forehead and brow bone reduction and biomaterials for forehead and brow bone augmentation are described. Innovations in bony reduction and implant augmentation methods are emphasized.[...]Thieme Medical Publishers...
Tue Oct 22, 2019 02:00
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