Significance of the Marginal Mandibular Branch in Relation to Facial Palsy Reconstruction: Assessment of Microanatomy and Macroanatomy Including Axonal Load in 96 Facial Halves.Significance of the Marginal Mandibular Branch in Relation to Facial Palsy Reconstruction: Assessment of Microanatomy and Macroanatomy Including Axonal Load in 96 Facial Halves. Ann Plast Surg. 2019 Sep 16;: Authors: Mandlik V, Ruewe M, Engelmann S, Geis S, Taeger C, Kehrer M, Tamm ER, Bleys R, Prantl L, Kehrer A Abstract BACKGROUND: The marginal mandibular branch (MMB) of the facial nerve provides l- Marjolin Ulcer: An Observational Epidemiological Study From a Tertiary Care Centre in India.Marjolin Ulcer: An Observational Epidemiological Study From a Tertiary Care Centre in India. Ann Plast Surg. 2019 Sep 16;: Authors: Chaturvedi G, Gupta AK, Das S, Gohil AJ, Lamba S Abstract INTRODUCTION: Marjolin ulcer is a rare skin malignancy. Marjolin ulcer arises in zones of long-standing scars, inflammation, or chronic wounds. MATERIAL AND METHODS: The present study was conducted to ass
- Upper Lip Reconstruction With Modification for Creating a Philtrum With Single-Stage Full-Thickness Skin Graft in Burned Face Injury.Upper Lip Reconstruction With Modification for Creating a Philtrum With Single-Stage Full-Thickness Skin Graft in Burned Face Injury. Ann Plast Surg. 2019 Sep 16;: Authors: Yen CI, Chen HC, Hsiao YC, Chang SY, Chuang SS Abstract BACKGROUND: The philtrum plays an important role in determining the shape and form of the upper lip and creates individual identity. Postburn scar contracture in this area of
- Book Review: Dermatologic Complications With Body Art: Tattoos, Piercings and Permanent Make-up.Book Review: Dermatologic Complications With Body Art: Tattoos, Piercings and Permanent Make-up. Ann Plast Surg. 2019 Sep 16;: Authors: Nagarajan M, Shokrollahi K PMID: 31567414 [PubMed - as supplied by publisher]
Dysphonia
- Continuous versus intermittent intraoperative neuromonitoring in complex benign thyroid surgery: A retrospective analysis and prospective follow-up.Related ArticlesContinuous versus intermittent intraoperative neuromonitoring in complex benign thyroid surgery: A retrospective analysis and prospective follow-up. Clin Otolaryngol. 2019 Sep 29;: Authors: Sedlmaier A, Steinmüller T, Hermanns M, Nawka T, Weikert S, Sedlmaier B, Caffier PP Abstract OBJECTIVES: To compare continuous (C-IONM) versus intermittent intraoperative neuromonitoring (I-IONM) i
- The Effect of Local Versus Intravenous Corticosteroids on the Likelihood of Dysphagia and Dysphonia Following Anterior Cervical Discectomy and Fusion: A Single-Blinded, Prospective, Randomized Controlled Trial.Related ArticlesThe Effect of Local Versus Intravenous Corticosteroids on the Likelihood of Dysphagia and Dysphonia Following Anterior Cervical Discectomy and Fusion: A Single-Blinded, Prospective, Randomized Controlled Trial. J Bone Joint Surg Am. 2018 Sep 05;100(17):1461-1472 Authors: Jenkins TJ, Nair R, Bhatt S, Rosenthal BD, Savage JW, Hsu WK, Patel AA Abstract BACKGROUND: Dysphagia and dysphonia
Journal of Pediatric Endocrinology and Metabolism.
- Long-term follow-up of children with classic congenital adrenal hyperplasia: suggestions for age dependent treatment in childhood and pubertyJournal Name: Journal of Pediatric Endocrinology and MetabolismIssue: Ahead of print
ScienceDirect Publication: European Journal of Vascular and Endovascular Surgery
- Achieving Thinned Blood: One Size Fits All?Publication date: Available online 1 October 2019Source: European Journal of Vascular and Endovascular SurgeryAuthor(s): Edward T.C. Choke, Jia S. Tay
- Adding Insight to Injury!Publication date: Available online 30 September 2019Source: European Journal of Vascular and Endovascular SurgeryAuthor(s): A.R. Naylor, J.D. Spence
- A Prospective Outcome Assessment After Bronchoscopic Interventions for Malignant Central Airway Obstruction.A Prospective Outcome Assessment After Bronchoscopic Interventions for Malignant Central Airway Obstruction. J Bronchology Interv Pulmonol. 2019 Sep 26;: Authors: Mohan A, Shrestha P, Madan K, Hadda V, Pandey RM, Upadhyay A, Khilnani GC, Guleria R Abstract BACKGROUND: A systematic assessment of comprehensive clinical outcomes after various therapeutic procedures for malignant central airway obstructi
- Comparison of nasal and olfactory functions between two surgical approaches for the treatment of concha bullosa: a randomised clinical trial.Related ArticlesComparison of nasal and olfactory functions between two surgical approaches for the treatment of concha bullosa: a randomised clinical trial. J Laryngol Otol. 2019 Sep 30;:1-5 Authors: Andaloro C, La Mantia I, Castro V, Grillo C Abstract OBJECTIVE: Concha bullosa may be associated with paranasal sinus infections and nasal obstruction. Middle concha mucosa membranes have olfactory neur
- Implementation of an Enhanced Recovery Protocol Is Associated With On-Time Initiation of Adjuvant Chemotherapy in Colorectal Cancer.Implementation of an Enhanced Recovery Protocol Is Associated With On-Time Initiation of Adjuvant Chemotherapy in Colorectal Cancer. Dis Colon Rectum. 2019 Sep 20;: Authors: Hassinger TE, Mehaffey JH, Martin AN, Bauer-Nilsen K, Turrentine FE, Thiele RH, Sarosiek BM, Reilley MJ, Hoang SC, Friel CM, Hedrick TL Abstract BACKGROUND: Delayed initiation of adjuvant chemotherapy negatively impacts long-term
- Mutational Profile in Vulvar, Vaginal, and Urethral Melanomas: Review of 37 Cases With Focus on Primary Tumor Site.Mutational Profile in Vulvar, Vaginal, and Urethral Melanomas: Review of 37 Cases With Focus on Primary Tumor Site. Int J Gynecol Pathol. 2019 Sep 20;: Authors: Zarei S, Voss JS, Jin L, Jenkins SM, Bryce AH, Erickson LA, Bell DA, Kipp BR, Flotte TJ Abstract Melanomas of female genital tract are rare tumors with poor prognosis. While BRAF-V600E is the most common pathogenic mutation seen in cutaneous
- Endocrinopathies and cancer cachexia.Endocrinopathies and cancer cachexia. Curr Opin Support Palliat Care. 2019 Sep 20;: Authors: Dev R, Del Fabbro E, Dalal S Abstract PURPOSE OF REVIEW: Cancer cachexia cannot be easily reversed by standard nutritional support and interventions directed at underlying metabolic derangements may be needed to prevent or reverse cachexia and maintain healthy body composition. The following review will highl
- Putting Exercise Into Oncology Practice: State-of-the-Science, Innovation, and Future Directions.Putting Exercise Into Oncology Practice: State-of-the-Science, Innovation, and Future Directions. Cancer J. 2019 Sep/Oct;25(5):316-319 Authors: Brown JC, Ligibel JA Abstract Physical activity provides numerous health benefits for cancer survivors. This review provides a summary of the evidence supporting the efficacy of physical activity to improve patient-reported and physiological outcomes during a
- The role of prostate-specific membrane antigen PET/computed tomography in primary staging of prostate cancer.The role of prostate-specific membrane antigen PET/computed tomography in primary staging of prostate cancer. Curr Opin Urol. 2019 Nov;29(6):569-577 Authors: Koschel S, Murphy DG, Hofman MS, Wong LM Abstract PURPOSE OF REVIEW: Functional imaging with PET combined with computed tomography (CT) is of emerging interest in prostate cancer (PCa). Development of prostate-specific membrane antigen (PSMA) ra
- Updates on intravesical therapy.Updates on intravesical therapy. Curr Opin Urol. 2019 Nov;29(6):649-655 Authors: Pisano F, Vila Reyes H, Breda A, Palou J Abstract PURPOSE OF REVIEW: To review the current literature concerning the intravesical treatment of nonmuscle invasive bladder cancer. RECENT FINDINGS: Bladder cancer is a high prevalent disease. Despite the recognized efficacy of traditional intravesical therapies, the
- Bleeding Assessment Scale in Critically Ill Children (BASIC): Physician-Driven Diagnostic Criteria for Bleeding Severity.Bleeding Assessment Scale in Critically Ill Children (BASIC): Physician-Driven Diagnostic Criteria for Bleeding Severity. Crit Care Med. 2019 Sep 25;: Authors: Nellis ME, Tucci M, Lacroix J, Spinella PC, Haque KD, Stock A, Steiner ME, Faustino EVS, Zantek ND, Davis PJ, Stanworth SJ, Cholette JM, Parker RI, Demaret P, Kneyber MCJ, Russell RT, Stricker PA, Vogel AM, Willems A, Josephson CD, Luban NLC, Loftis LL, Leteurt
- Efficacy of Adoptive Therapy with Tumor-infiltrating Lymphocytes and Recombinant Interleukin-2 in Advanced Cutaneous Melanoma: A Systematic Review and Meta-analysis.Efficacy of Adoptive Therapy with Tumor-infiltrating Lymphocytes and Recombinant Interleukin-2 in Advanced Cutaneous Melanoma: A Systematic Review and Meta-analysis. Ann Oncol. 2019 Sep 30;: Authors: Dafni U, Michielin O, Lluesma SM, Tsourti Z, Polydoropoulou V, Karlis D, Besser MJ, Haanen J, Svane IM, Ohashi PS, Kammula US, Orcurto A, Zimmermann S, Trueb L, Klebanoff CA, Lotze MT, Kandalaft LE, Coukos G Abst
- Art as a Learning Tool: Medical Student Perspectives on Implementing Visual Art into Histology Education.Related ArticlesArt as a Learning Tool: Medical Student Perspectives on Implementing Visual Art into Histology Education. Cureus. 2019 Jul 23;11(7):e5207 Authors: Cracolici V, Judd R, Golden D, Cipriani NA Abstract Creating visual art to teach and learn histologic concepts is uncommon. A pilot visual art program was developed for use in first-year medical student courses that include histology with t
- Rituximab increases the risk of hepatitis B virus reactivation in non-Hodgkin lymphoma patients who are hepatitis B surface antigen-positive or have resolved hepatitis B virus infection in a real-world setting: a retrospective study.Related ArticlesRituximab increases the risk of hepatitis B virus reactivation in non-Hodgkin lymphoma patients who are hepatitis B surface antigen-positive or have resolved hepatitis B virus infection in a real-world setting: a retrospective study. PeerJ. 2019;7:e7481 Authors: Tsai YF, Yang CI, Du JS, Lin MH, Tang SH, Wang HC, Cho SF, Liu YC, Su YC, Dai CY, Hsiao HH Abstract Background: Hepatitis B
- Chondrosarcoma: the impact of comorbidity - 30 years of experience from a population-based database including 199 consecutive chondrosarcoma patients.Related ArticlesChondrosarcoma: the impact of comorbidity - 30 years of experience from a population-based database including 199 consecutive chondrosarcoma patients. Orthop Res Rev. 2019;11:109-116 Authors: Aggerholm-Pedersen N, Maretty-Nielsen K, Baerentzen S, Jørgensen PH, Hansen BH, Baad-Hansen T, Keller J, Safwat A Abstract Background: Adjustment for comorbidity when investigating potential prog
- Adherence to Active Surveillance Protocols for Low-risk Prostate Cancer: Results of the Movember Foundation's Global Action Plan Prostate Cancer Active Surveillance Initiative.Related ArticlesAdherence to Active Surveillance Protocols for Low-risk Prostate Cancer: Results of the Movember Foundation's Global Action Plan Prostate Cancer Active Surveillance Initiative. Eur Urol Oncol. 2019 Sep 26;: Authors: Kalapara AA, Verbeek JFM, Nieboer D, Fahey M, Gnanapragasam V, Van Hemelrijck M, Lee LS, Bangma CH, Steyerberg EW, Harkin T, Helleman J, Roobol MJ, Frydenberg M, Movember Foundation’s Globa
- Challenges in a Technology-Based Cancer Pain Management Program Among Asian American Breast Cancer Survivors.Related ArticlesChallenges in a Technology-Based Cancer Pain Management Program Among Asian American Breast Cancer Survivors. Comput Inform Nurs. 2019 05;37(5):243-249 Authors: Im EO, Ji X, Kim S, Chee E, Bao T, Mao JJ, Chee W Abstract This paper aims to discuss the challenges faced during a pilot study that tested a technology-based cancer pain management program among Asian American survivors of br
- Radiation Therapy Patient Education Review and a Case Study Using the Virtual Environment for Radiotherapy Training System.Related ArticlesRadiation Therapy Patient Education Review and a Case Study Using the Virtual Environment for Radiotherapy Training System. J Med Imaging Radiat Sci. 2018 03;49(1):106-117 Authors: Jimenez YA, Lewis SJ Abstract INTRODUCTION: The emergence of modern learning environments for radiation therapy (RT) education offers innovative opportunities for RT patients. This article presents a descri
- Current and Future Management of Malignant Mesothelioma: A Consensus Report from the National Cancer Institute Thoracic Malignancy Steering Committee, International Association for the Study of Lung Cancer, and Mesothelioma Applied Research Foundation.Related ArticlesCurrent and Future Management of Malignant Mesothelioma: A Consensus Report from the National Cancer Institute Thoracic Malignancy Steering Committee, International Association for the Study of Lung Cancer, and Mesothelioma Applied Research Foundation. J Thorac Oncol. 2018 11;13(11):1655-1667 Authors: Tsao AS, Lindwasser OW, Adjei AA, Adusumilli PS, Beyers ML, Blumenthal GM, Bueno R, Burt BM, Carbone M
- A Convolutional Neural Network Uses Microscopic Images to Differentiate between Mouse and Human Cell Lines and Their Radioresistant Clones.Related ArticlesA Convolutional Neural Network Uses Microscopic Images to Differentiate between Mouse and Human Cell Lines and Their Radioresistant Clones. Cancer Res. 2018 12 01;78(23):6703-6707 Authors: Toratani M, Konno M, Asai A, Koseki J, Kawamoto K, Tamari K, Li Z, Sakai D, Kudo T, Satoh T, Sato K, Motooka D, Okuzaki D, Doki Y, Mori M, Ogawa K, Ishii H Abstract : Artificial intelligence (AI) tr
- Recommendations for Research and Practice to Improve Work Outcomes Among Cancer Survivors.Related ArticlesRecommendations for Research and Practice to Improve Work Outcomes Among Cancer Survivors. J Natl Cancer Inst. 2018 10 01;110(10):1041-1047 Authors: de Moor JS, Alfano CM, Kent EE, Norton WE, Coughlan D, Roberts MC, Grimes M, Bradley CJ Abstract Major knowledge gaps limit the development and implementation of interventions to improve employment outcomes among people with cancer. To id
- Usability Testing of an Interactive Communication Tool to Help Children Express Their Cancer Symptoms.Related ArticlesUsability Testing of an Interactive Communication Tool to Help Children Express Their Cancer Symptoms. J Pediatr Oncol Nurs. 2018 Sep/Oct;35(5):320-331 Authors: Tsimicalis A, Rennick J, Stinson J, May SL, Louli J, Choquette A, Treherne S, Berube S, Yoon S, Ruland C Abstract OBJECTIVE: Sisom is an interactive computer software program that allows children to rate the severity of their
- The Screening, Diagnosis, Treatment, and Follow-Up of Breast Cancer.Related ArticlesThe Screening, Diagnosis, Treatment, and Follow-Up of Breast Cancer. Dtsch Arztebl Int. 2018 05 04;115(18):316-323 Authors: Wöckel A, Albert US, Janni W, Scharl A, Kreienberg R, Stüber T Abstract BACKGROUND: Breast cancer is the most common cancer in women. The German S3 guideline of 2012 has now been updated to take account of advances in the early detection, diagnostic evaluation, t
- Epigenetic Reprogramming Strategies to Reverse Global Loss of 5-Hydroxymethylcytosine, a Prognostic Factor for Poor Survival in High-grade Serous Ovarian Cancer.Related ArticlesEpigenetic Reprogramming Strategies to Reverse Global Loss of 5-Hydroxymethylcytosine, a Prognostic Factor for Poor Survival in High-grade Serous Ovarian Cancer. Clin Cancer Res. 2018 03 15;24(6):1389-1401 Authors: Tucker DW, Getchell CR, McCarthy ET, Ohman AW, Sasamoto N, Xu S, Ko JY, Gupta M, Shafrir A, Medina JE, Lee JJ, MacDonald LA, Malik A, Hasselblatt KT, Li W, Zhang H, Kaplan SJ, Murphy GF, Hir
- A Tale of Two Concepts: Harmonizing the Free Radical and Antagonistic Pleiotropy Theories of Aging.Related ArticlesA Tale of Two Concepts: Harmonizing the Free Radical and Antagonistic Pleiotropy Theories of Aging. Antioxid Redox Signal. 2018 10 01;29(10):1003-1017 Authors: Golubev A, Hanson AD, Gladyshev VN Abstract SIGNIFICANCE: The two foremost concepts of aging are the mechanistic free radical theory (FRT) of how we age and the evolutionary antagonistic pleiotropy theory (APT) of why we age. B
Case Reports in Infectious Diseases
- Pulmonary Infections Caused by Emerging Pathogenic Species of NocardiaPulmonary infections are the most common clinical manifestations of Nocardia species. There is an increase in cases of nocardial infections occurring worldwide attributable to the increase in the immunosuppressed population. The availability of molecular methods has aided the detection of more number of cases as well as unusual species. Still, it remains one of the most underdiagnosed pathogens. Recognition of drug resistance in this organism has
Case Reports in Ophthalmology
- Vitreoretinal Surgery and Panretinal Photocoagulation in a Patient with Multiple Large Retinal Capillary Hemangiomas (von Hippel-Lindau Disease): A Novel ApproachThe authors present a novel surgical approach for the treatment of retinal capillary hemangiomas (RCHs) secondary to von Hippel-Lindau (VHL) disease. This is a case report of a 23-year-old male patient with VHL that presented with multiple large RCHs and a thick epiretinal membrane (ERM) in his left eye, with best-corrected visual acuity (BCVA) of 20/80. This condition was surgically addressed with 23-gauge pars plana vitrectomy, ERM and internal
- Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy.Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy. J Vis Exp. 2019 Sep 12;(151): Authors: Ruggeri FS, Šneideris T, Chia S, Vendruscolo M, Knowles TPJ Abstract The phenomenon of protein misfolding and aggregation results in the formation of highly heterogeneous protein aggregates, which are associated with neurodegenerative conditions such as Alzheim
- Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis.Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis. J Vis Exp. 2019 Sep 14;(151): Authors: Moye SL, Diaz-Castro B, Gangwani MR, Khakh BS Abstract Astrocytes are essential components of neural circuits. They tile the entire central nervous system (CNS) and are involved in a variety of functions, which include neurotransmitter clearance, ion regulation, synaptic modulation, metabolic s
- Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis.Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis. J Vis Exp. 2019 Sep 11;(151): Authors: Dagur RS, Wang W, Makarov E, Sun Y, Poluektova LY Abstract Despite the increased life expectancy of patients infected with human immunodeficiency virus-1 (HIV-1), liver disease has emerged as a common cause of their morbidity. The liver immunopathology caused by HIV-1 r
- DNAzyme-dependent Analysis of rRNA 2'-O-Methylation.DNAzyme-dependent Analysis of rRNA 2'-O-Methylation. J Vis Exp. 2019 Sep 16;(151): Authors: Winczura K, Grzechnik P Abstract Guide box C/D small nucleolar RNAs (snoRNAs) catalyze 2'-O-methylation of ribosomal and small nuclear RNA. However, a large number of snoRNA in higher eukaryotes may promiscuously recognize other RNA species and 2'-O-methylate multiple targets. Here, we provide step-by-step gui
- Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection.Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection. J Vis Exp. 2019 Sep 14;(151): Authors: Dutta D, Heo I, O'Connor R Abstract Cryptosporidium parvum is one of the major causes of human diarrheal disease. To understand the pathology of the parasite and develop efficient drugs, an in vitro culture system that recapitulates the conditions in the hos
- Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate.Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate. J Vis Exp. 2019 Sep 13;(151): Authors: Kim S, Martinez-Santiesteban F, Scholl TJ Abstract The fundamental limit to in vivo imaging applications of hyperpolarized 13C-enriched compounds is their finite spin-lattice relaxation times. Various factors affect the relaxation rates, such as buffer composition,
- An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells.An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells. J Vis Exp. 2019 Sep 12;(151): Authors: Philp L, Chan H, Rouzbahman M, Rostami A, Ding L, Bratman SV, Akens MK, Irish JC, Bernardini MQ, Zheng G Abstract Endometrial cancer is the most common gynecologic malignancy in North America and the incidence is rising worldwide. Trea
- An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis.An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis. J Vis Exp. 2019 Sep 12;(151): Authors: Qi X, Schepers E, Avella D, Kimchi ET, Kaifi JT, Staveley-O'Carroll KF, Li G Abstract The absence of a clinically relevant animal model addressing the typical immune characteristics of hepatocellular cancer (HCC) has signif
- A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones.A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones. J Vis Exp. 2019 Sep 16;(151): Authors: Marek A, Schüler C, Satué M, Haigl B, Erben RG Abstract RNA yield and integrity are decisive for RNA analysis. However, it is often technically challenging to maintain RNA integrity throughout the entire laser capture microdissection (LCM) procedure. Since LCM st
- CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors.CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors. J Vis Exp. 2019 Sep 14;(151): Authors: Jin Y, Shen Y, Su X, Weintraub N, Tang Y Abstract Duchenne muscular dystrophy (DMD) is a severe progressive muscle disease caused by mutations in the dystrophin gene, which ultimately leads to the exhaustion of muscle progenitor cells. Clustered regularly interspaced sh
- Imaging of Extracellular Vesicles by Atomic Force Microscopy.Imaging of Extracellular Vesicles by Atomic Force Microscopy. J Vis Exp. 2019 Sep 11;(151): Authors: Skliar M, Chernyshev VS Abstract Exosomes and other extracellular vesicles (EVs) are molecular complexes consisting of a lipid membrane vesicle, its surface decoration by membrane proteins and other molecules, and diverse luminal content inherited from a parent cell, which includes RNAs, proteins, and
- Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems.Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems. J Vis Exp. 2019 Sep 13;(151): Authors: Šimek K, Sirova D Abstract Elucidating trophic interactions, such as predation and its effects, is a frequent task for many researchers in ecology. The study of microbial communities has many limitations, and determining a predator, prey, and predato
- A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression.A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression. J Vis Exp. 2019 Sep 13;(151): Authors: Lee D, Erickson A, Dudley AT, Ryu S Abstract Mechanical stimuli are known to modulate biological functions of cells and tissues. Recent studies have suggested that compressive stress alters growth plate cartilage architecture and results in growth modulation of long bones of children.
- A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene.A Robust Polymerase Chain Reaction-based Assay for Quantifying Cytosine-guanine-guanine Trinucleotide Repeats in Fragile X Mental Retardation-1 Gene. J Vis Exp. 2019 Sep 16;(151): Authors: Wang H, Zhu X, Gui B, Cheung WC, Shi M, Yang Z, Kwok KY, Lim R, Pietilä S, Zhu Y, Choy KW Abstract Fragile X syndrome (FXS) and associated disorders are caused by expansion of the cytosine-guanine-guanine (CGG) tri
- Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs.Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs. J Vis Exp. 2019 Sep 16;(151): Authors: van Dijk EL, Eleftheriou E, Thermes C Abstract The study of small RNAs (sRNAs) by next-generation sequencing (NGS) is challenged by bias issues during library preparation. Several types of sRNA such as plant microRNAs (miRNAs) carry a 2'-O-methyl (2'-OMe) modification at their 3' termin
- Purification of a High Molecular Mass Protein in Streptococcus mutans.Purification of a High Molecular Mass Protein in Streptococcus mutans. J Vis Exp. 2019 Sep 14;(151): Authors: Murata T, Yamashita M, Ishikawa M, Shibuya K, Hanada N Abstract Elucidation of a gene's function typically involves comparison of phenotypic traits of wild-type strains and strains in which the gene of interest has been disrupted. Loss of function following gene disruption is subsequently res
- In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research.In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research. J Vis Exp. 2019 Sep 16;(151): Authors: Wischhusen JC, Wilson KE Abstract Monoclonal antibodies (mAbs) are important tools in cancer detection, diagnosis, and treatment. They are used to unravel the role of proteins in tumorigenesis, can be directed to cancer biomarkers ena
- Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon.Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon. J Vis Exp. 2019 Sep 13;(151): Authors: Durovic T, Ninkovic J Abstract Electroporation is a transfection method in which an electrical field is applied to cells to create temporary pores in a cell membrane and increase its permeability, thereby allowing different molecules to be introduced to the cell. In
- Purification of High Yield Extracellular Vesicle Preparations Away from Virus.Purification of High Yield Extracellular Vesicle Preparations Away from Virus. J Vis Exp. 2019 Sep 12;(151): Authors: DeMarino C, Barclay RA, Pleet ML, Pinto DO, Branscome H, Paul S, Lepene B, El-Hage N, Kashanchi F Abstract One of the major hurdles in the field of extracellular vesicle (EV) research today is the ability to achieve purified EV preparations in a viral infection setting. The presented
- Investigating the Effect of Visual Imagery and Learning Shape-Audio Regularities on Bouba and Kiki.Investigating the Effect of Visual Imagery and Learning Shape-Audio Regularities on Bouba and Kiki. J Vis Exp. 2019 Sep 13;(151): Authors: Graven T, Desebrock C Abstract This article presents a protocol for investigating the role of visual imagery in the bouba/kiki-effect, whether training in noticing the bouba/kiki shape-audio regularities affects the bouba/kiki-effect and the recognition of individ
- Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin.Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin. J Vis Exp. 2019 Sep 12;(151): Authors: Mathur R, Alam MM, Zhao XF, Huang Y, Zhu X Abstract Significant studies have been carried out to understand effective management of intestinal fibrosis. However, the lack of better knowledge of fibrosis has hindered the development of
- Investigation into Deep Breathing through Measurement of Ventilatory Parameters and Observation of Breathing Patterns.Investigation into Deep Breathing through Measurement of Ventilatory Parameters and Observation of Breathing Patterns. J Vis Exp. 2019 Sep 16;(151): Authors: Yokogawa M, Kurebayashi T, Soma K, Miaki H, Nakagawa T Abstract In this protocol, two deep breathing patterns were shown to 15 participants to determine an easy yet effective method of breathing exercise for future application in a clinical sett
- Evaluating Skilled Prehension in Mice Using an Auto-Trainer.Evaluating Skilled Prehension in Mice Using an Auto-Trainer. J Vis Exp. 2019 Sep 12;(151): Authors: Hubbard R, Dunthorn J, O'Brien RJ, Tasch D, Tasch U, Zeiler SR Abstract We describe a method to introduce naïve mice to a novel prehension (reach-to-grasp) task. Mice are housed singly in cages with a frontal slot that permits the mouse to reach out of its cage and retrieve food pellets. Minimal food r
- Cortisol Extraction from Sturgeon Fin and Jawbone Matrices.Cortisol Extraction from Sturgeon Fin and Jawbone Matrices. J Vis Exp. 2019 Sep 10;(151): Authors: Ghassemi Nejad J, Ataallahi M, Salmanzadeh MH, Park KT, Lee HG, Shoae A, Rahimi A, Sung KI, Park KH Abstract The aims of this study were to develop a technique for the extraction of cortisol from sturgeon fins using two washing solvents (water and isopropanol) and quantify any differences in fin cortiso
- Sandy Soil Improvement through Microbially Induced Calcite Precipitation (MICP) by Immersion.Sandy Soil Improvement through Microbially Induced Calcite Precipitation (MICP) by Immersion. J Vis Exp. 2019 Sep 12;(151): Authors: Liu S, Du K, Wen K, Huang W, Amini F, Li L Abstract The goal of this article is to develop an immersion method to improve the microbially induced calcite precipitation (MICP) treated samples. A batch reactor was assembled to immerse soil samples into cementation media.
- Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice.Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice. J Vis Exp. 2019 Sep 16;(151): Authors: Britson KA, Black AD, Wagner KR, Lloyd TE Abstract Treatment effects observed in animal studies often fail to be recapitulated in clinical trials. While this problem is multifaceted, one reason for this failure is the use of inadequate laboratory models. It is challenging to model complex human
- Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas.Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas. J Vis Exp. 2019 Sep 13;(151): Authors: Boscolo Sesillo F, Sacco A Abstract Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children. Although significant efforts have enabled the identification of common mutations associated with RMS and allowed discrimination of different RMS subtype
- Experimental Methods to Study Human Postural Control.Experimental Methods to Study Human Postural Control. J Vis Exp. 2019 Sep 11;(151): Authors: Amiri P, Mohebbi A, Kearney R Abstract Many components of the nervous and musculoskeletal systems act in concert to achieve the stable, upright human posture. Controlled experiments accompanied by appropriate mathematical methods are needed to understand the role of the different sub-systems involved in human
- Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy.Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy. J Vis Exp. 2019 Sep 16;(151): Authors: Harmer J, Struve N, Brüning-Richardson A Abstract Drug discovery and development in cancer research is increasingly being based on drug screens in a 3D format. Novel inhibitors targeting the migratory and invasive potential of cancer ce
- Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue.Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue. J Vis Exp. 2019 Sep 10;(151): Authors: Abu Abed U, Brinkmann V Abstract Neutrophil granulocytes, also called polymorphonuclear leukocytes (PMN) due to their lobulated nucleus, are the most abundant type of leukocytes. They mature in the bone marrow and are released into the peripheral blood, w
- Evaluation of the Productivity of Social Wasp Colonies (Vespinae) and an Introduction to the Traditional Japanese Vespula Wasp Hunting Technique.Evaluation of the Productivity of Social Wasp Colonies (Vespinae) and an Introduction to the Traditional Japanese Vespula Wasp Hunting Technique. J Vis Exp. 2019 Sep 11;(151): Authors: Saga T Abstract For vespine wasps, colony productivity is typically estimated by counting the number of larval cells. This paper presents an improved method that enables researchers to estimate more accurately the numb
- upper respiratory tract infection; +93 new citations93 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: upper respiratory tract infection These pubmed results were generated on 2019/10/01PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
- upper respiratory tract infection; +93 new citations93 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: upper respiratory tract infection These pubmed results were generated on 2019/10/01PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
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