This study aimed to develop a questionnaire for measuring patient perceptions of participating in clinical trials. Development was based on earlier research on patient views of involvement in medical care and ...
Previous studies showed that flight muscles degenerate after migration in some aphid species; however, the underlying molecular mechanism remains virtually unknown. In this study, using the wheat aphid, Sitobion ...
Khat is an herbal cultivated plant growing mainly in Eastern Africa and Arabians. Chronic khat chewing leads to the development of liver toxicity, cardiovascular disease, sleeping disorder, psychosis, memory i...
For the past 10 years, refugees from Eritrea represented the majority of asylum seekers in Switzerland. However, data on their health status remains limited. In this cross-sectional survey followed by a 1-year...
We described the dynamics of cholera in Tanzania between 2007 and 2017 and assessed the weaknesses of the current surveillance system in providing necessary data in achieving the global roadmap to 2030 for cho...
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Related ArticlesSurgical Treatment of Bilateral Tibia Deformity in a 9-Year-Old Child Suffering from Osteogenesis Imperfecta Type III: A Case Report. Am J Case Rep. 2019 Oct 21;20:1540-1550 Authors: Delniotis I, Leidinger B Abstract BACKGROUND Osteogenesis imperfecta is a rare inherited connective tissue disorder that is mainly characterized by long bone deformities and increased susceptibility to bone fractures. The aim of this study was to present...
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Related ArticlesMolecular Imaging for Radiotherapy Planning and Response Assessment for Cervical Cancer. Semin Nucl Med. 2019 Nov;49(6):493-500 Authors: Lin AJ, Dehdashti F, Grigsby PW Abstract Positron emission tomography (PET) with [18F]fluorodeoxyglucose (FDG) leverages imaging contrast from accelerated and aberrant glucose metabolism, which is a hallmark of cancer. FDG-PET is essential for assessing involved lymph nodes and distant metastases for...
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Related ArticlesDifferential Release of Exocytosis Marker Dyes Indicates Stimulation-Dependent Regulation of Synaptic Activity. Front Neurosci. 2019;13:1047 Authors: Henkel AW, Mouihate A, Welzel O Abstract There is a general consensus that synaptic vesicular release by a full collapse process is the primary machinery of synaptic transmission. However, competing view suggests that synaptic vesicular release operates via a kiss-and-run mechanism. By monitoring...
Related ArticlesPre- and Post-natal High Fat Feeding Differentially Affects the Structure and Integrity of the Neurovascular Unit of 16-Month Old Male and Female Mice. Front Neurosci. 2019;13:1045 Authors: Contu L, Nizari S, Heath CJ, Hawkes CA Abstract Compelling experimental and clinical evidence supports a role for maternal obesity in offspring health. Adult children of obese mothers are at greater risk of obesity, diabetes, coronary heart disease...
Related ArticlesStructural Connectivity Alterations in Amyotrophic Lateral Sclerosis: A Graph Theory Based Imaging Study. Front Neurosci. 2019;13:1044 Authors: Fortanier E, Grapperon AM, Le Troter A, Verschueren A, Ridley B, Guye M, Attarian S, Ranjeva JP, Zaaraoui W Abstract Background: Amyotrophic lateral sclerosis (ALS) is a relentlessly progressive neurodegenerative disorder. Diffusion magnetic resonance imagining (MRI) studies have consistently...
Related ArticlesIncreased Maternal Prenatal Adiposity, Inflammation, and Lower Omega-3 Fatty Acid Levels Influence Child Negative Affect. Front Neurosci. 2019;13:1035 Authors: Gustafsson HC, Holton KF, Anderson AN, Nousen EK, Sullivan CA, Loftis JM, Nigg JT, Sullivan EL Abstract Objective: Increased maternal adiposity during pregnancy is associated with offspring risk for psychiatric disorders. Inflammation secondary to adiposity is believed to be an...
Related ArticlesEnzymatic Digestion of Hyaluronan-Based Brain Extracellular Matrix in vivo Can Induce Seizures in Neonatal Mice. Front Neurosci. 2019;13:1033 Authors: Balashova A, Pershin V, Zaborskaya O, Tkachenko N, Mironov A, Guryev E, Kurbatov L, Gainullin M, Mukhina I Abstract It is well-known that hyaluronic acid (HA) as a component of brain extracellular matrix (ECM) plays a pivotal role in the nervous system and is involved in synaptic plasticity...
Related ArticlesμECoG Recordings Through a Thinned Skull. Front Neurosci. 2019;13:1017 Authors: Brodnick SK, Ness JP, Richner TJ, Thongpang S, Novello J, Hayat M, Cheng KP, Krugner-Higby L, Suminski AJ, Ludwig KA, Williams JC Abstract The studies described in this paper for the first time characterize the acute and chronic performance of optically transparent thin-film micro-electrocorticography (μECoG) grids implanted on a thinned skull as both an electrophysiological...
Related ArticlesSleep Problems and Workplace Violence: A Systematic Review and Meta-Analysis. Front Neurosci. 2019;13:997 Authors: Magnavita N, Di Stasio E, Capitanelli I, Lops EA, Chirico F, Garbarino S Abstract Background: This systematic review with meta-analysis was carried out to study the relationship between workplace violence and sleep problems. Methods: The PRISMA statement was used to conduct a systematic search of the literature on PubMed/MEDLINE,...
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Publication date: Available online 21 October 2019Source: Pathology - Research and PracticeAuthor(s): Won Jin Cho, Jung-Soo PyoAbstractObjectiveWe conducted experiments to elucidate the impact of ischemic change on benign prostatic hyperplasia (BPH) using immunohistochemistry.MethodsMedical records of consecutive patients over 60 years of age who underwent transurethral resection of the prostate for BPH between January 2009 and September 2012 were evaluated. As vascular risk factors, the presence...
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Related ArticlesRecording Obligatory Cortical Auditory Evoked Potentials in Infants: Quantitative Information on Feasibility and Parent Acceptability. Ear Hear. 2019 Oct 16;: Authors: Munro KJ, Purdy SC, Uus K, Visram A, Ward R, Bruce IA, Marsden A, Stone MA, Van Dun B Abstract OBJECTIVES: With the advent of newborn hearing screening and early intervention, there is a growing interest in using supra-threshold obligatory cortical auditory evoked potentials...
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Related ArticlesAn Association between Right Ventricular Dysfunction and Sudden Cardiac Death. Heart Rhythm. 2019 Oct 18;: Authors: Pandat S, Nagaura T, Nair SG, Uy-Evanado A, Stecker E, Nichols GA, Jui J, Shiota T, Chugh SS, Reinier K Abstract BACKGROUND: The effectiveness of severely reduced left ventricular ejection fraction (LVEF <35%) as a predictor of sudden cardiac death (SCD) has diminished, and improvements in risk stratification await discovery...
Long-term efficacy and safety of phrenic nerve stimulation for the treatment of central sleep apnea.
Related ArticlesLong-term efficacy and safety of phrenic nerve stimulation for the treatment of central sleep apnea. Sleep. 2019 Oct 21;42(11): Authors: Fox H, Oldenburg O, Javaheri S, Ponikowski P, Augostini R, Goldberg LR, Stellbrink C, Mckane S, Meyer TE, Abraham WT, Costanzo MR Abstract STUDY OBJECTIVE: To evaluate long-term efficacy and safety of phrenic nerve stimulation (PNS) in patients with moderate-to-severe central sleep apnea (CSA) through...
Related ArticlesThe effect of acute exposure to morphine on breathing variability and cardiopulmonary coupling in men with obstructive sleep apnea: A randomized controlled trial. J Sleep Res. 2019 Oct 21;:e12930 Authors: Wu JG, Wang D, Rowsell L, Wong KK, Yee BJ, Nguyen CD, Han F, Hilmisson H, Thomas RJ, Grunstein RR Abstract Opioid-related deaths from respiratory depression are increasing but there is only limited information on the effect of morphine...
Related ArticlesAnti-IgLON5 Disease: A Case With 11-Year Clinical Course and Review of the Literature. Front Neurol. 2019;10:1056 Authors: Nissen MS, Blaabjerg M Abstract Background: Anti-IgLON5 disease is a novel disorder with a complex interplay between inflammation and neurodegeneration. Patients develop antibodies against IgLON5 but also deposition of neuronal tau protein. Symptoms often have an insidious onset, slow progression and mimic other neurological...
Related ArticlesObstructive sleep apnea and left ventricular hypertrophy: More questions than answers. J Clin Hypertens (Greenwich). 2019 Oct 21;: Authors: Cuspidi C, Sala C, Tadic M PMID: 31631522 [PubMed - as supplied by publisher]
Related Articles[Tonsillitis, pharyngitis and OSAS in children - symptoms, clinical diagnostics and therapy]. MMW Fortschr Med. 2019 Oct;161(18):54-57 Authors: Widmann M, Jakob M PMID: 31631298 [PubMed - in process]
Bone metabolism parameters and inactive matrix Gla protein in patients with obstructive sleep apnea.
Related ArticlesBone metabolism parameters and inactive matrix Gla protein in patients with obstructive sleep apnea. Sleep. 2019 Oct 21;: Authors: Vilovic M, Dogas Z, Ticinovic Kurir T, Borovac JA, Supe-Domic D, Vilovic T, Ivkovic N, Rusic D, Novak A, Bozic J Abstract STUDY OBJECTIVES: The aim of this study was to investigate differences in Dual-energy X-ray absorptiometry (DXA) parameters, trabecular bone score (TBS), bone turnover markers and inactive...
Related ArticlesThe impact of obstructive sleep apnea and PAP therapy on all-cause and cardiovascular mortality based on age and gender - a literature review. Respir Investig. 2019 Oct 17;: Authors: Mashaqi S, Gozal D Abstract BACKGROUND: Obstructive sleep apnea (OSA) is a common sleep disorder which negatively impacts different body systems, especially the cardiovascular system. The correlation between sleep related breathing disorders and cardiovascular...
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Publication date: Available online 22 October 2019Source: Clinical TherapeuticsAuthor(s): Paul Krogstad, Royce Johnson, Maria I. Garcia-Lloret, Arash Heidari, Manish J. ButteABSTRACTPurposeCoccidioidomycosis (CM) is a systemic fungal disease caused by the dimorphic fungi Coccidioides immitis and Coccidioides posadasii. In its endemic areas of the United States, CM is growing as a public health challenge with a marked increase in incidence in the last 15 years. Although Coccidioides infection is asymptomatic...
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Related ArticlesApplications for Open Source Microplate-Compatible Illumination Panels. J Vis Exp. 2019 Oct 03;(152): Authors: Baillargeon P, Spicer TP, Scampavia L Abstract Microplates are commonly used in the modern laboratory environment for a wide variety of tasks both in small-scale laboratory benchtop operations as well as large-scale high-throughput screening (HTS) campaigns. Though laboratory automation has greatly increased the utility of microplates...
Related ArticlesOperation of the Collaborative Composite Manufacturing (CCM) System. J Vis Exp. 2019 Oct 01;(152): Authors: Li P, Zhang X, Xie W, Hoa SV Abstract The automated tape placement and the automated fiber placement (AFP) machines provide a safer working environment and reduce the labor intensity of workers than the traditional manual fiber placement does. Thus, the production accuracy, repeatability and efficiency of composite manufacturing...
Related ArticlesDeveloping Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions. J Vis Exp. 2019 Oct 05;(152): Authors: Mir AQ, Dolui D, Khandelwal S, Bhatt H, Kumari B, Barman S, Kanvah S, Dutta A Abstract Developing photocatalytic H2 production devices is the one of the key steps for constructing a global H2-based renewable energy infrastructure. A number of photoactive assemblies have emerged where a photosensitizer...
Related ArticlesFabrication and Implementation of a Reference-Free Traction Force Microscopy Platform. J Vis Exp. 2019 Oct 06;(152): Authors: Banda OA, Slater JH Abstract Quantifying cell-induced material deformation provides useful information concerning how cells sense and respond to the physical properties of their microenvironment. While many approaches exist for measuring cell-induced material strain, here we provide a methodology for monitoring...
Related ArticlesA Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products. J Vis Exp. 2019 Oct 04;(152): Authors: Murphy KM, Chung S, Fogla S, Minsky HB, Zhu KY, Zerbe P Abstract Diterpenoids form a diverse class of small molecule natural products that are widely distributed across the kingdoms of life and have critical biological functions in developmental processes, interorganismal interactions, and environmental...
Related ArticlesBacterial Peptide Display for the Selection of Novel Biotinylating Enzymes. J Vis Exp. 2019 Oct 03;(152): Authors: Granhøj J, Dimke H, Svenningsen P Abstract Biotin is an attractive post-translational modification of proteins that provides a powerful tag for the isolation and detection of protein. Enzymatic biotinylation by the E. coli biotin-protein ligase BirA is highly specific and allows for the biotinylation of target proteins in...
Related ArticlesCharacterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy. J Vis Exp. 2019 Oct 04;(152): Authors: Bhaskaran V, Peruzzi P Abstract The biological relevance of microRNAs (miRNAs) in health and disease significantly relies on specific combinations of many simultaneously deregulated miRNAs rather than the action of a single miRNA. The characterization of these specific...
Related ArticlesNative Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization. J Vis Exp. 2019 Oct 06;(152): Authors: Wang M, Hoo Lim K, Chow KT Abstract Interferon regulatory factor 5 (IRF5) is a key transcription factor for regulating the immune response. It is activated downstream of the Toll-like receptor myeloid differentiation primary response gene 88 (TLR-MyD88) signaling pathway. IRF5 activation involves phosphorylation,...
Related ArticlesMethod Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper. J Vis Exp. 2019 Oct 04;(152): Authors: Kombolias M, Obrzut J, Postek MT, Poster DL, Obeng YS Abstract The current analytical techniques for characterizing printing and graphic arts substrates are largely ex situ and destructive. This limits the amount of data that can be obtained from an individual sample and renders it difficult to...
Related ArticlesMeasuring Neural Mechanisms Underlying Sleep-Dependent Memory Consolidation During Naps in Early Childhood. J Vis Exp. 2019 Oct 02;(152): Authors: Allard T, Riggins T, Ewell A, Weinberg B, Lokhandwala S, Spencer RMC Abstract Sleep is critical for daily functioning. One important function of sleep is the consolidation of memories, a process that makes them stronger and less vulnerable to interference. The neural mechanisms underlying the...
Related ArticlesIron Nanowire Fabrication by Nano-Porous Anodized Aluminum and its Characterization. J Vis Exp. 2019 Oct 06;(152): Authors: Patel NS, Lago-Cachón D, Mohammed H, Moreno JA, Kosel J Abstract Magnetic nanowires possess unique properties that have attracted the interest of different fields of research, including basic physics, biomedicine, and data storage. We demonstrate a fabrication method for iron (Fe) nanowires via electrochemical deposition...
Related ArticlesLentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models. J Vis Exp. 2019 Oct 03;(152): Authors: Sano S, Wang Y, Evans MA, Yura Y, Sano M, Ogawa H, Horitani K, Doviak H, Walsh K Abstract Manipulating genes in hematopoietic stem cells using conventional transgenesis approaches can be time-consuming, expensive, and challenging. Benefiting from advances in genome editing technology and lentivirus-mediated...
Related ArticlesStandardizing a Non-Lethal Method for Characterizing the Reproductive Status and Larval Development of Freshwater Mussels (Bivalvia: Unionida). J Vis Exp. 2019 Oct 04;(152): Authors: Beaver CE, Geda SR, Johnson NA Abstract Actively monitoring the timing, development, and reproductive patterns of endangered species is critical when managing for population recovery. Freshwater mussels are among the most imperiled organisms in the world,...
Related ArticlesPerturbing Endothelial Biomechanics via Connexin 43 Structural Disruption. J Vis Exp. 2019 Oct 04;(152): Authors: Islam MM, Steward RL Abstract Endothelial cells have been established to generate intercellular stresses and tractions, but the role gap junctions play in endothelial intercellular stress and traction generation is currently unknown. Therefore, we present here a mechanics-based protocol to probe the influence of gap junction...
Related ArticlesIntegration of Brain Tissue Saturation Monitoring in Cardiopulmonary Exercise Testing in Patients with Heart Failure. J Vis Exp. 2019 Oct 01;(152): Authors: Huang SC, Chen CP, Fu TC, Chen YJ Abstract Cerebral hypo-oxygenation during rest or exercise negatively impacts the exercise capacity of patients with heart failure with reduced ejection fraction (HF). However, in clinical cardiopulmonary exercise testing (CPET), cerebral hemodynamics...
Related ArticlesA Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons. J Vis Exp. 2019 Oct 06;(152): Authors: Wenzel DA, Kunzmann BC, Steinhorst NA, Spitzer MS, Schultheiss M Abstract Experimental research on corneal endothelial cells is associated with several difficulties. Human donor corneas are scarce and rarely available for experimental investigations as they are normally needed for transplantation. Endothelial cell cultures...
Related ArticlesA Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency. J Vis Exp. 2019 Oct 05;(152): Authors: Theile M, Wiora L, Russ D, Reuter J, Ishikawa H, Schwerk C, Schroten H, Mogk S Abstract Transepithelial/endothelial electrical resistance (TEER) has been used since the 1980s to determine confluency and permeability of in vitro barrier model systems. In most cases, chopstick electrodes...
Related ArticlesA Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression. J Vis Exp. 2019 Oct 06;(152): Authors: van der Linden AJ, Yelleswarapu M, Pieters PA, Swank Z, Huck WTS, Maerkl SJ, de Greef TFA Abstract The limitations of cell-based synthetic biology are becoming increasingly apparent as researchers aim to develop larger and more complex synthetic genetic regulatory circuits. The analysis of synthetic genetic...
Related ArticlesUsing Flexible Gold-Titanium Reaction Cells to Simulate Pressure-Dependent Microbial Activity in the Context of Subsurface Biomining. J Vis Exp. 2019 Oct 05;(152): Authors: Ostertag-Henning C, Zhang R, Helten O, Weger T, Schippers A Abstract Laboratory studies investigating subsurface microbial processes, such as metal leaching in deep ore deposits (biomining), share common and challenging obstacles, including the special environmental...
Related ArticlesPrecision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response. J Vis Exp. 2019 Oct 03;(152): Authors: Richey JA, Sullivan-Toole H, Strege M, Carlton C, McDaniel D, Komelski M, Epperley A, Zhu H, Allen IC Abstract Minimal erythema dose (MED) testing is frequently used in clinical settings for determining the smallest amount of ultraviolet (UV) irradiation necessary to produce...
Related ArticlesChronic Implantation of Multiple Flexible Polymer Electrode Arrays. J Vis Exp. 2019 Oct 04;(152): Authors: Chung JE, Joo HR, Smyth CN, Fan JL, Geaghan-Breiner C, Liang H, Liu DF, Roumis D, Chen S, Lee KY, Pebbles JA, Tooker AC, Tolosa VM, Frank LM Abstract Simultaneous recordings from large populations of individual neurons across distributed brain regions over months to years will enable new avenues of scientific and clinical development....
Related ArticlesImplantation of an Isoproterenol Mini-Pump to Induce Heart Failure in Mice. J Vis Exp. 2019 Oct 03;(152): Authors: Ren S, Chang S, Tran A, Mandelli A, Wang Y, Wang JJ Abstract Isoproterenol (ISO), is a non-selective beta-adrenergic agonist, that is used widely to induce cardiac injury in mice. While the acute model mimics stress-induced cardiomyopathy, the chronic model, administered through an osmotic pump, mimics advanced heart failure...
Related ArticlesFacile Synthesis of Colloidal Lead Halide Perovskite Nanoplatelets via Ligand-Assisted Reprecipitation. J Vis Exp. 2019 Oct 01;(152): Authors: Ha SK, Tisdale WA Abstract In this work, we demonstrate a facile method for colloidal lead halide perovskite nanoplatelet synthesis (Chemical formula: L2[ABX3]n-1BX4, L: butylammonium and octylammonium, A: methylammonium or formamidinium, B: lead, X: bromide and iodide, n: number of [BX6]4- octahedral...
Related ArticlesGeneration of Multicellular Human Primary Endometrial Organoids. J Vis Exp. 2019 Oct 04;(152): Authors: Murphy AR, Wiwatpanit T, Lu Z, Davaadelger B, Kim JJ Abstract The human endometrium is one of the most hormonally responsive tissues in the body and is essential for the establishment of pregnancy. This tissue can also become diseased and cause morbidity and even death. Model systems to study human endometrial biology have been limited...
Related ArticlesDissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration. J Vis Exp. 2019 Oct 03;(152): Authors: Gonzalez Malagon SG, Dobson L, Muñoz AML, Dawson M, Barrell W, Marangos P, Krause M, Liu KJ Abstract Over the past several decades there has been an increased availability of genetically modified mouse models used to mimic human pathologies. However, the...
Related ArticlesGeneration of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture. J Vis Exp. 2019 Oct 03;(152): Authors: Sakib S, Yu Y, Voigt A, Ungrin M, Dobrinski I Abstract Organoids are three dimensional structures composed of multiple cell types that are capable of recapitulating tissue architecture and functions of organs in vivo. Formation of organoids has opened up different avenues of basic and translational...
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Related ArticlesAdditional venous anastomosis in free profunda artery perforator flap transfer using the posterior accessory saphenous vein. J Plast Reconstr Aesthet Surg. 2019 Sep 24;: Authors: Iida T, Yoshimatsu H, Karakawa R, Kanayama K, Harima M, Okazaki M Abstract BACKGROUND: The profunda artery perforator (PAP) flap has recently been widely used for head and neck as well as breast reconstruction. Although this flap has various advantages, its vascular...
Related ArticlesRelation between palatal index and the incidence of postoperative fistula in patients with complete unilateral cleft palate (a prospective cohort study). J Plast Reconstr Aesthet Surg. 2019 Oct 01;: Authors: Obad Saleh H, Nasr KM, Abdull Sattar AH, Amr K PMID: 31631003 [PubMed - as supplied by publisher]
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Related ArticlesSupra-auricular and post-auricular resection of pre-auricular and post-auricular sinuses. J Laryngol Otol. 2019 Oct 21;:1-6 Authors: Ding H, Xie H, Qiao F, Wang T, Huang X, Ning Q Abstract OBJECTIVE: This study aimed to classify the pre-auricular sinus before performing radical dissection, so as to achieve optimal aesthetic results. METHODS: The recent five-year clinical data of 53 patients with a congenital pre-auricular sinus...
Related ArticlesEffect of chronic suppurative otitis media on distortion product otoacoustic emission input-output functions in conventional and ultra-high frequencies. J Laryngol Otol. 2019 Oct 21;:1-10 Authors: Kirubaharane SI, Palani S, Alexander A, Sreenivasan A Abstract BACKGROUND: Detection and valid measurements of distortion product otoacoustic emissions are not influenced by cochlear status alone, but also by middle-ear status. There is a need...
Related ArticlesA comprehensive review of otorhinolaryngological global health concerns. J Laryngol Otol. 2019 Oct 21;:1-6 Authors: Murphy DC Abstract BACKGROUND: ENT disease prevalence, risk factors and treatment vary between developed and developing countries. Health provision, particularly disease prevention strategies and surgery, in developing countries is poor, manifesting as a high frequency of common and preventable diseases. Healthcare systems...
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