When did gastro-esophageal reflux become a disease? A historical perspective on GER(D) nomenclature.
Related Articles When did gastro-esophageal reflux become a disease? A historical perspective on GER(D) nomenclature. Int J Pediatr Otorhinolaryngol. 2020 Jul 02;137:110214 Authors: Rameau A, Mudry A Abstract BACKGROUND: Within the span of a few decades, gastro-esophageal reflux (GER) evolved from a rare entity to the most commonly diagnosed upper gastro-intestinal disease, i.e. gastro-esophageal reflux disease (GERD). The boundaries of GERD...
Tue Jul 14, 2020 18:15
Related Articles Emotional and behavioral problems in children and adolescents with hearing loss and their effects on quality of life. Int J Pediatr Otorhinolaryngol. 2020 Jul 10;137:110245 Authors: Yigider AP, Yilmaz S, Ulusoy H, Kara T, Kufeciler L, Kaya KH Abstract PURPOSE: Determination of mental functions in children with hearing loss is important in improving the quality of life of the child. Many scales have been developed for this purpose....
Tue Jul 14, 2020 18:15
Related Articles Bilateral cochlear implantation versus unilateral cochlear implantation in deaf children: Effects of sentence context and listening conditions on recognition of spoken words in sentences. Int J Pediatr Otorhinolaryngol. 2020 Jul 10;137:110237 Authors: Lee Y, Sim H Abstract OBJECTIVES: Previous studies have investigated the efficacy of bilateral cochlear implants (CIs) in deaf children. The current study focused on the use of...
Tue Jul 14, 2020 18:15
Related Articles Study on the correlation between OSAS and thoracic deformity in children: A retrospective single-center study in China. Int J Pediatr Otorhinolaryngol. 2020 Jul 11;137:110226 Authors: Wang YG, Lin C, Ye SN, Xu YT, Lin XH, Ke XY, Zheng JX, Chen Y, Chen GH Abstract OBJECTIVE: To evaluate the correlation between obstructive sleep apnea syndrome (OSAS) and the development of thoracic deformity in Children. METHODS: A retrospective...
Tue Jul 14, 2020 18:15
Related Articles Grisel's syndrome post otolaryngology procedures: A systematic review. Int J Pediatr Otorhinolaryngol. 2020 Jun 27;137:110225 Authors: Al-Driweesh T, Altheyab F, Alenezi M, Alanazy S, Aldrees T Abstract OBJECTIVE: To increase awareness and the index of suspicion regarding Grisel's syndrome among otolaryngologists after otolaryngology procedures. METHOD: We conducted a search of MEDLINE/PubMed for articles published...
Tue Jul 14, 2020 18:15
Related Articles Social and religious attitudes of Jordanian parents toward children born with orofacial clefts. Int J Pediatr Otorhinolaryngol. 2020 Jun 26;137:110222 Authors: Alfwaress F, Alomari M Abstract BACKGROUND AND GOALS: Parental attitudes towards orofacial clefts (OFC) can be influenced by culture. Interestingly, there are no studies up to date examined parental attitudes towards these congenital anomalies in Jordan. The objectives...
Tue Jul 14, 2020 18:15
Related Articles Buccal fat pad in cleft palate repair- An institutional experience of 27 cases. Int J Pediatr Otorhinolaryngol. 2020 Jul 11;137:110218 Authors: Saralaya S, Desai AK, Ghosh R Abstract OBJECTIVES: The purpose of this paper is to consider the anatomical basis and surgical technique along with the utility of buccal fat pad (BFP) for the reconstruction in cleft lip and palate patients. METHODS: We reviewed 27 cases of CLAP...
Tue Jul 14, 2020 18:15
Related Articles Profile of childhood hearing loss in the Western Cape, South Africa. Int J Pediatr Otorhinolaryngol. 2020 Jul 11;137:110248 Authors: Kuschke S, Swanepoel W, le Roux T, Strauss S Abstract OBJECTIVES: To describe the nature, associated risk factors and age of diagnosis for childhood hearing loss in a South African cohort from the Western Cape Province. METHODS: A retrospective review of clinical data from children under...
Tue Jul 14, 2020 18:15
Related Articles Predictive epidemiological and clinical factors of nasopharyngeal carcinoma diagnosis: Adult versus pediatric population. Int J Pediatr Otorhinolaryngol. 2020 Jul 03;137:110203 Authors: Hasnaoui M, Lahmar R, Ben Mabrouk A, Masmoudi M, Mighri K, Driss N Abstract OBJECTIVE: The objective of this study is to compare the clinical presentation and the cancer staging of nasopharyngeal carcinoma (NPC) between pediatric and adult populations...
Tue Jul 14, 2020 18:15
Related Articles Follow up of a birth cohort to identify prevalence and risk factors for otitis media among Indian children in the eighth year of life. Int J Pediatr Otorhinolaryngol. 2020 Jul 10;137:110201 Authors: Samson D, Rupa V, Veeraraghavan B, Varghese R, Isaac R, L J Abstract BACKGROUND: Otitis media, a disease highly prevalent among children worldwide, manifests clinically in both acute and chronic forms. The manner and time at which...
Tue Jul 14, 2020 18:15
Related Articles Juvenile recurrent respiratory papillomatosis treated with combined erlotinib and celecoxib: Initial report. Int J Pediatr Otorhinolaryngol. 2020 Jul 10;137:110194 Authors: Rojas-Lechuga MJ, Remacha J, González-Sánchez N, Grau JJ, Castillo P, Haag O, Vilaseca I Abstract Recurrent respiratory papillomatosis (RRP) is a chronic disease caused by human papillomavirus (HPV). RRP is a clinical challenge because of the high recurrence...
Tue Jul 14, 2020 18:15
Related Articles Correlation of cochlear nerve cross-sectional area and auditory performance after cochlear implantation in prelingual children with bilateral profound hearing loss. Int J Pediatr Otorhinolaryngol. 2020 Jul 02;137:110173 Authors: Jain S, Sharma V, Patro SK, Khera P, Yadav T, Tiwari S, Kaushal D, Soni K, Goyal A Abstract OBJECTIVE: To assess whether cochlear nerve (CN) cross-sectional area as measured with parasagittal magnetic...
Tue Jul 14, 2020 18:15
Related Articles Addition of dexmedetomidine to ropivacaine for local infiltration anaesthesia improves analgesic efficacy after tonsillectomy and adenoidectomy: A randomized controlled trial. Int J Pediatr Otorhinolaryngol. 2020 Jul 03;137:110168 Authors: Hao J, Wu Z, Luo Z, Dong B Abstract OBJECTIVES: The aim of this study was to determine whether the addition of dexmedetomidine to ropivacaine for local infiltration anaesthesia was more effective...
Tue Jul 14, 2020 18:15
Related Articles A meta-analysis on surgery with or without postoperative radiotherapy to treat squamous cell esophageal carcinoma. Int J Surg. 2020 Jul 10;: Authors: Lin HN, Chen LQ, Shang QX Abstract BACKGROUND: Esophageal cancer (EC) is the sixth most common cause of cancer-related mortality in the world. Currently, surgery is the recommended treatment modality when possible. The outcomes of surgery alone are poor, and postoperative...
Tue Jul 14, 2020 14:57
Related Articles Learning curve of kidney transplantation in a high-volume center: Cohort study. Int J Surg. 2020 Jul 10;: Authors: Takagi K, Outmani L, Kimenai HJAN, Terkivatan T, Tran KTC, Ijzermans JNM, Minnee RC Abstract BACKGROUND: The purpose of this study was to evaluate surgical outcomes of kidney transplantation (KTX) based on surgeon volume and surgeon experience, and to develop the learning curve model for KTX using the cumulative...
Tue Jul 14, 2020 14:57
Related Articles Contra-lateral desynchronized alpha oscillations linearly correlate with discrimination performance of tactile acuity. J Neural Eng. 2020 Jul 13;: Authors: Su S, Chai G, Sheng X, Meng J, Zhu X Abstract OBJECTIVE: We used EEG to investigate cortical oscillatory activities during the tactile discrimination task and characterize the correlation between the EEG features and subjects' discrimination performance. APPROACH:...
Tue Jul 14, 2020 17:37
Related Articles In vivo feasibility of epiretinal stimulation using ultrananocrystalline diamond electrodes. J Neural Eng. 2020 Jul 13;: Authors: Shivdasani MN, Evans M, Burns O, Yeoh J, Allen PJ, Nayagam D, Villalobos J, Abbott CJ, Luu CD, Opie NL, Sabu A, Saunders AL, McPhedran M, Cardamone L, McGowan C, Maxim V, Williams RA, Fox K, Cicione R, Garrett DJ, Ahnood A, Ganesan K, Meffin H, Burkitt AN, Prawer S, Williams CE, Shepherd RK Abstract ...
Tue Jul 14, 2020 17:37
Related Articles A systematic review of antipsychotic agents for primary delusional infestation. J Dermatolog Treat. 2020 Jul 13;:1-47 Authors: McPhie ML, Kirchhof MG Abstract Background: Delusional infestation (DI) is a rare delusional disorder in which individuals have a false belief that they are infested with bugs, parasites, or insects, despite the lack of medical evidence that such an infestation exists. Data on the effectiveness of antipsychotics...
Tue Jul 14, 2020 17:56
Related Articles Vehicles for atopic dermatitis therapies: more than just a placebo. J Dermatolog Treat. 2020 Jul 10;:1-50 Authors: Danby SG, Draelos ZD, Stein Gold LF, Cha A, Vlahos B, Aikman L, Sanders P, Wu-Linhares D, Cork MJ Abstract A topical vehicle is a "carrier system" for an active pharmaceutical (or cosmetic) substance, referred to hereafter as the drug, but a vehicle may also be used on its own as an emollient to ameliorate dry skin....
Tue Jul 14, 2020 13:51
Related Articles A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks. J Vis Exp. 2020 Jun 26;(160): Authors: Meister TL, Klöhn M, Steinmann E, Todt D Abstract Hepatitis E virus is the leading cause of liver cirrhosis and liver failure with increasing prevalence worldwide. The single-stranded RNA virus is predominantly transmitted by blood transfusions, inadequate sanitary conditions and contaminated food products. To...
Tue Jul 14, 2020 17:38
Related Articles Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics. J Vis Exp. 2020 Jun 29;(160): Authors: Malic L, Elmanzalawy A, Daoud J, Geissler M, Boutin A, Lukic L, Janta M, Da Fonte D, Nassif C, Veres T Abstract A multiplexed droplet PCR (mdPCR) workflow and detailed protocol for determining epigenetic-based white blood cell (WBC) differential count is described, along...
Tue Jul 14, 2020 17:38
Related Articles Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures. J Vis Exp. 2020 Jun 26;(160): Authors: Kumar S, Liu Y, Taylor RE Abstract Current strategies in DNA and RNA nanotechnology enable the self-assembly of a variety of nucleic acid nanostructures in aqueous or substantially hydrated media. In this article, we describe detailed protocols that enable the construction...
Tue Jul 14, 2020 17:38
Related Articles Erratum: Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings. J Vis Exp. 2020 Jul 13;(161): Authors: Abstract An erratum was issued for: Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings. Step 2.1.3 in the Protocol was corrected. Step 2.1.3 in the Protocol was updated from: Add final concentrations of glucose and MgCl, then stir until dissolved (Table 1, solution...
Tue Jul 14, 2020 17:38
Related Articles Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis. J Vis Exp. 2020 Jun 27;(160): Authors: Liao W, Ma C, Zhang N Abstract Tissue-resident memory T cell (TRM) is a rapidly expanding field of immunology research. Isolating T cells from various non-lymphoid tissues is one of the key steps to investigate TRMs. There are slight variations in lymphocyte isolation protocols for different organs. Kidney...
Tue Jul 14, 2020 17:38
Related Articles Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia. J Vis Exp. 2020 Jun 26;(160): Authors: Zhang B, Joseph B, Saatman KE, Chen L Abstract Neural stem cell (NSC) therapy is an emerging innovative treatment for stroke, traumatic brain injury and neurodegenerative disorders. As compared to intracranial delivery, intra-arterial administration of NSCs is less invasive and...
Tue Jul 14, 2020 17:38
Related Articles Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice. J Vis Exp. 2020 Jun 27;(160): Authors: Wang M, Huang Y, Gu W, Wang H Abstract T Follicular Helper (Tfh) cells are an independent CD4+ T cell subset specialized in providing help for germinal center (GC) development and generation of high-affinity antibodies. In influenza virus infection, robust Tfh and GC B cell...
Tue Jul 14, 2020 17:38
Related Articles A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development. J Vis Exp. 2020 Jun 24;(160): Authors: Juber BA, Elgin TG, Fricke EM, Gong H, Reese J, McElroy SJ Abstract Chorioamnionitis is a common precipitant of preterm birth and is associated with many of the morbidities of prematurity, including necrotizing enterocolitis (NEC). However, a mechanistic...
Tue Jul 14, 2020 17:38
Related Articles Optimization of the Cuff Technique for Murine Heart Transplantation. J Vis Exp. 2020 Jun 26;(160): Authors: Ma Y, Xie B, Dai H, Wang C, Liu S, Lan T, Xu S, Yan G, Qi Z Abstract Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to improve surgical efficiency. In our lab, we have optimized the cuff technique with two major steps....
Tue Jul 14, 2020 17:38
Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers.
Related Articles Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers. J Vis Exp. 2020 Jun 24;(160): Authors: Wakula M, Balcerak A, Smietanka U, Chmielarczyk M, Konopiński R, Grzybowska EA Abstract The methods presented here quantify some parameters of confluent adherent cell monolayers from multiple appropriately stained confocal images: adhesion to the substrate as a function of the number...
Tue Jul 14, 2020 17:38
Related Articles Flow Cytometry Approach to Characterize Phagocytic Properties of Acutely-Isolated Adult Microglia and Brain Macrophages In Vitro. J Vis Exp. 2020 Jun 23;(160): Authors: Ramesha S, Rayaprolu S, Rangaraju S Abstract Microglia and central nervous system (CNS)-infiltrating macrophages, collectively called CNS mononuclear phagocytes (CNS-MPs), play central roles in neurological diseases including neurodegeneration and stroke....
Tue Jul 14, 2020 17:38
Related Articles Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches. J Vis Exp. 2020 Jun 25;(160): Authors: Shirakabe K, Higashiyama M, Shibuya N, Horiuchi K, Saruta M, Hokari R Abstract Naïve lymphocytes recirculate from the blood to the lymphoid tissues under physiological condition and it is commonly recognized as an important phenomenon in the gut immunity. The stroma of secondary lymphoid organs, such as Peyer's...
Tue Jul 14, 2020 17:38
Related Articles Detection of Phytophthora capsici in Irrigation Water using Loop-Mediated Isothermal Amplification. J Vis Exp. 2020 Jun 25;(160): Authors: Hudson O, Waliullah S, Hand J, Gazis-Seregina R, Baysal-Gurel F, Ali ME Abstract Phytophthora capsici is a devastating oomycete pathogen that affects many important solanaceous and cucurbit crops causing significant economic losses in vegetable production annually. Phytophthora capsici...
Tue Jul 14, 2020 17:38
Related Articles Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis. J Vis Exp. 2020 Jun 25;(160): Authors: Hsu CC, Tsai CF, Tao WA, Wang P Abstract Protein phosphorylation is crucial for the regulation of enzyme activity and gene expression under osmotic condition. Mass spectrometry (MS)-based phosphoproteomics has transformed the way of studying plant signal transduction. However, requirement of lots of starting...
Tue Jul 14, 2020 17:38
Related Articles Visualization of DNA Repair Proteins Interaction by Immunofluorescence. J Vis Exp. 2020 Jun 26;(160): Authors: de la Peña Avalos B, Dray E Abstract Mammalian cells are constantly exposed to chemicals, radiations, and naturally occurring metabolic by-products, which create specific types of DNA insults. Genotoxic agents can damage the DNA backbone, break it, or modify the chemical nature of individual bases. Following DNA...
Tue Jul 14, 2020 17:38
Related Articles Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method. J Vis Exp. 2020 Jun 24;(160): Authors: Eski SE, Dubois C, Singh SP Abstract High-throughput transcriptome and epigenome profiling requires preparation of a single cell or single nuclei suspension. Preparation of the suspension with intact cell or nuclei involves dissociation and permeabilization, steps that can introduce unwanted noise and undesirable...
Tue Jul 14, 2020 17:38
Related Articles Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery. J Vis Exp. 2020 Jun 23;(160): Authors: Nie L, Sun S, Sun M, Zhou Q, Zhang Z, Zheng L, Wang L Abstract Due to drug resistance and toxicity in healthy cells, use of doxorubicin (DOX) has been limited in clinical cancer therapy. This protocol describes the designing of poly(ethylenimine) grafted with polyethylene...
Tue Jul 14, 2020 17:38
Related Articles Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis. J Vis Exp. 2020 Jun 27;(160): Authors: Showpnil IA, Miller KR, Taslim C, Pishas KI, Lessnick SL, Theisen ER Abstract Many cancers are characterized by chromosomal translocations which result in the expression of oncogenic fusion transcription factors. Typically, these proteins contain an intrinsically...
Tue Jul 14, 2020 17:38
Related Articles Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC. J Vis Exp. 2020 Jun 26;(160): Authors: Gaugler P, Gaugler V, Kamleitner M, Schaaf G Abstract The phosphate esters of myo-inositol, also termed inositol phosphates (InsPs), are a class of cellular regulators playing important roles in plant physiology. Due to their negative charge, low abundance and...
Tue Jul 14, 2020 17:38
Related Articles Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining. J Vis Exp. 2020 Jun 23;(160): Authors: Kim H, Xue X Abstract Oxidative stress is an important event under both physiological and pathological conditions. In this study, we demonstrate how to quantify oxidative stress by measuring total reactive oxygen species (ROS) using 2',7'-dichlorodihydrofluorescein diacetate...
Tue Jul 14, 2020 17:38
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